Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,466,056	A→T	8.2%	noncoding (104/119 nt)	PP_mr39 ←	P15 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,466,056	0	A	T	8.2%	196.7 / 4.5	69	noncoding (104/119 nt)	PP_mr39	P15 ncRNA
Reads supporting (aligned to +/- strand):  ref base A (33/29);  new base T (3/3);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGGAGGCGGCAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGG  >  NC_002947/3465909‑3466197                                                                                                                                                    |                                                                                                                                              ttCGGAGGCGGCAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGaa                                                                                                                                              <  7:135966/148‑1 (MQ=255) aTCGGAGGCGGCAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGaa                                                                                                                                              <  4:166294/149‑1 (MQ=255)          ggcAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAg                                                                                                                                             <  8:289198/141‑1 (MQ=255)          ggcAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAg                                                                                                                                             >  7:289198/1‑141 (MQ=255)           gcAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCggg                                                                                                                                    <  5:7670/149‑1 (MQ=255)            cAGTCTGGCCCGACCGGGATTGGCTAAATCGCCAGGGGTAGGTGTTTTCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGCTTCCCGACCgggg                                                                                                                                   <  8:252684/149‑1 (MQ=255)                 tGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGGGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGttt                                                                                                                               >  5:151765/1‑148 (MQ=255)                 tGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGttt                                                                                                                               >  4:321736/1‑148 (MQ=255)                   gCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAg                                                                                                                            >  1:177536/1‑149 (MQ=255)                     ccGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTa                                                                                                                          >  6:357735/1‑149 (MQ=255)                       gAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCTTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGa                                                                                                                             >  4:326521/1‑144 (MQ=255)                       gAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTTGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGa                                                                                                                             <  3:326521/144‑1 (MQ=255)                        aaCGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATtc                                                                                                                       >  3:274054/1‑149 (MQ=255)                             gATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACTCCCGGTCGGGAAGCCGACCGGGGGTTTGa                                                                                                                             >  1:63625/1‑138 (MQ=255)                             gATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACTCCCGGTCGGGAAGCCGACCGGGGGTTTGa                                                                                                                             <  2:63625/138‑1 (MQ=255)                                  gCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcgg                                                                                                             >  3:291120/1‑149 (MQ=255)                                      aaTCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCggcggc                                                                                                         <  1:323669/149‑1 (MQ=255)                                       aTCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGcc                                                                                                        <  1:179898/149‑1 (MQ=255)                                          gCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCt                                                                                                     >  1:42888/1‑149 (MQ=255)                                               gCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAg                                                                                                <  4:291120/149‑1 (MQ=255)                                                cGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAgg                                                                                               <  7:336186/149‑1 (MQ=255)                                                    ggTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGa                                                                                           <  6:151765/149‑1 (MQ=255)                                                        tttGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGcc                                                                                       >  1:268091/1‑149 (MQ=255)                                                          tGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGCAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCgg                                                                                     >  8:76852/1‑149 (MQ=255)                                                          tGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCgg                                                                                     >  5:129614/1‑149 (MQ=255)                                                             gtagtaATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCgggtg                                                                                  >  3:50601/1‑149 (MQ=255)                                                              tagtaATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCgggtgg                                                                                 <  7:205495/149‑1 (MQ=255)                                                              tagtaATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCgggtgg                                                                                 <  3:86364/149‑1 (MQ=255)                                                                   ataaGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATc                                                                            >  2:68492/1‑149 (MQ=255)                                                                   ataaGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATc                                                                            >  5:368715/1‑149 (MQ=255)                                                                          gCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGcc                                                                      >  5:15859/1‑148 (MQ=255)                                                                            gTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAg                                                                   >  3:142601/1‑149 (MQ=255)                                                                             tAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGt                                                                  <  6:368715/149‑1 (MQ=255)                                                                             tAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGt                                                                  >  1:317791/1‑149 (MQ=255)                                                                                 tCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGc                                                               >  8:94076/1‑148 (MQ=255)                                                                                   aTGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTa                                                             <  2:177536/148‑1 (MQ=255)                                                                                    tGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTAGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTaaa                                                           >  8:287735/1‑149 (MQ=255)                                                                                      aaTCTGTCCTCAAAGTGAATCGGTGTTGGTCAATGGCCTGAAAAAGAAAACCCCCGGTCGTGAAGCGGACCGGGGGTTTGAGTATGCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAAc                                                          <  5:313262/148‑1 (MQ=255)                                                                                      aaTCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAAc                                                          <  1:68492/148‑1 (MQ=255)                                                                                               tctatgtgtATCTGTGTTGGTTAATGGCCTGAAAACCAAAACCCCGGGTGGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTGGATGGTGGACGCCGGGTGGGTATCGGCGCCTAGTg                                                                 <  8:121829/127‑1 (MQ=255)                                                                                               tCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCa                                                 >  3:271525/1‑148 (MQ=255)                                                                                               tCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGAATTCTCATGTTCGGCGGCACCTCGAAGGTGGACGCCGGGTGGGTATCGTCGCCTAGTg                                                                 >  7:121829/1‑132 (MQ=255)                                                                                                 aaaGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAAc                                                          <  1:25019/137‑1 (MQ=255)                                                                                                 aaaGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAAc                                                          >  2:25019/1‑137 (MQ=255)                                                                                                 aaaGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCaaa                                               <  4:50601/148‑1 (MQ=255)                                                                                                 aaaGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAg                                              <  3:236839/149‑1 (MQ=255)                                                                                                     tGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAAt                                          <  4:271525/149‑1 (MQ=255)                                                                                                      gAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTaa                                           >  2:358297/1‑147 (MQ=255)                                                                                                         gcGGTGTTGGTCAAAGTCCTGAAAAACATAACCCCCGGTGGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCt                                      <  2:182196/148‑1 (MQ=255)                                                                                                          cGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGgcgc                                                                       >  3:77663/1‑115 (MQ=255)                                                                                                          cGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGgcgc                                                                       <  4:77663/115‑1 (MQ=255)                                                                                                            gtgtTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTgg                                    >  2:268969/1‑148 (MQ=255)                                                                                                              gtTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAg                                 >  7:124260/1‑149 (MQ=255)                                                                                                                 ggTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTg                              >  5:218501/1‑149 (MQ=255)                                                                                                                   tCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGcc                            <  5:357735/149‑1 (MQ=255)                                                                                                                   tCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGcc                            <  1:268969/149‑1 (MQ=255)                                                                                                                     aaaGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCAc                          <  7:94076/149‑1 (MQ=255)                                                                                                                     aaaGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGCGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCAc                          <  2:42888/149‑1 (MQ=255)                                                                                                                          ccTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGc                     <  3:321736/149‑1 (MQ=255)                                                                                                                              aaaaaCAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCgg                  >  5:239135/1‑148 (MQ=255)                                                                                                                                  ctcaaacCCCCGGTCGGCTTCCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCaa                                                       >  6:68280/4‑107 (MQ=38)                                                                                                                                  ctcaaacCCCCGGTCGGCTTCCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCaa                                                       <  5:68280/104‑1 (MQ=38)                                                                                                                                    aaaacCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGt                                             <  7:280946/115‑1 (MQ=255)                                                                                                                                    aaaacCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGt                                             >  8:280946/1‑115 (MQ=255)                                                                                                                                    aaaacCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGc           >  3:20106/1‑149 (MQ=255)                                                                                                                                      aacCCCCGGTCGGCTTCCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGc           >  3:9718/1‑147 (MQ=255)                                                                                                                                         ccccGGTCGGCTTCCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTgg                                    >  8:166274/1‑119 (MQ=255)                                                                                                                                         ccccGGTCGGCTTCCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTgg                                    <  7:166274/119‑1 (MQ=255)                                                                                                                                          cccGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGca      >  6:99527/1‑148 (MQ=255)                                                                                                                                          cccGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCAGGCAACGGCCAGca      >  8:179702/1‑148 (MQ=255)                                                                                                                                             ggTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAgg  <  2:317791/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                              ATCGGAGGCGGCAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGG  >  NC_002947/3465909‑3466197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑