Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,554,460 G→C 7.5% E26Q (GAG→CAG)  PP_3139 → group 1 family glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,554,4600GC 7.5% 206.2 / 6.5 67E26Q (GAG→CAG) PP_3139group 1 family glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (30/32);  new base C (2/3);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

CCCGTAGTCGACAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGTTGTTGGCACCGACGTGGCAGGTACT  >  NC_002947/3554312‑3554608
                                                                                                                                                    |                                                                                                                                                    
cccGTAGTCGACAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTgg                                                                                                                                                      >  7:57390/1‑149 (MQ=255)
      gTCGACAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTCCAgcgc                                                                                                                                                 <  8:178660/148‑1 (MQ=255)
       tCGACAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGcc                                                                                                                                                >  3:84638/1‑148 (MQ=255)
         gACAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAg                                                                                                                                             <  2:287948/149‑1 (MQ=255)
         gACAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTCCAGCGCCag                                                                                                                                              <  5:27519/148‑2 (MQ=255)
          aCAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAgg                                                                                                                                            <  2:113814/149‑1 (MQ=255)
           cAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAggg                                                                                                                                           <  8:101118/149‑1 (MQ=255)
           cAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAggg                                                                                                                                           <  3:203400/149‑1 (MQ=255)
                  aagtgaagGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCTGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAgg                                                                                                                                    <  3:3326/149‑1 (MQ=255)
                  aagtgaagGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAg                                                                                                                                     <  1:38035/148‑1 (MQ=255)
                   agttaagGTTTCCAGGGAAGGTTTGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGt                                                                                                                                   <  8:57390/149‑1 (MQ=255)
                    gtgaagGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTg                                                                                                                                  <  6:213330/149‑1 (MQ=255)
                    gtgaagGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTg                                                                                                                                  >  7:23014/1‑149 (MQ=255)
                      gaagGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGc                                                                                                                                 >  8:156803/1‑148 (MQ=255)
                         ggTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACg                                                                                                                             <  7:156803/149‑1 (MQ=255)
                             gCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGta                                                                                                                           >  7:91320/1‑147 (MQ=255)
                                        ttGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCgtgg                                                                                                              <  8:141509/149‑1 (MQ=255)
                                           ggcatcaAGCGGGTTTGGATCTGGATTCGTATGCTCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCCCAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCgtgggtg                                                                                                           <  8:308804/147‑1 (MQ=255)
                                            tcatcaAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCgtgggtgg                                                                                                          <  3:131708/149‑1 (MQ=255)
                                              atcaAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGa                                                                                                        >  2:360390/1‑149 (MQ=255)
                                                   gCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTAGCGAAATACTGGCGCTGGAGCGCCAAGGGCTTGAGGTGCAACGTATAGACATGCGTGGGTGGGAAGcc                                                                                                    >  4:244318/1‑148 (MQ=255)
                                                      ggTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAg                                                                                                <  3:162704/149‑1 (MQ=255)
                                                           ggATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCa                                                                                            <  1:284732/148‑1 (MQ=255)
                                                            gATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAg                                                                                           <  4:166753/148‑1 (MQ=255)
                                                                 ggATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCTACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGcc                                                                                     >  3:344217/1‑149 (MQ=255)
                                                                  gATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGcc                                                                                                                      <  6:176740/115‑1 (MQ=255)
                                                                  gATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGcc                                                                                                                      >  5:176740/1‑115 (MQ=255)
                                                                  gATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGAGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAAc                                                                                                                              <  8:166970/107‑1 (MQ=255)
                                                                  gATTCGTATGCGCATTGCTTACTTCATCAAACAGTACCCGAAAGTCAGCCACACTATCATCCGTCGCGAAATACTGGCGCAGGAGCGCCATGGCGGTGAGGTGCTGCGTATAGCCCTGCGTGGGTGGGACGCCTCAGTGCAGGATGcc                                                                                     >  2:311353/1‑148 (MQ=255)
                                                                  gATTCGTATGCGCATTGCTTACTTAATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGTGGTTGAGGTGCAAc                                                                                                                              >  7:166970/1‑107 (MQ=255)
                                                                          tGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGc                                                                             >  3:76819/1‑148 (MQ=255)
                                                                            cgcATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAg                                                                          <  2:298986/149‑1 (MQ=255)
                                                                               aTTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCg                                                                        >  8:147197/1‑148 (MQ=255)
                                                                                   cTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACg                                                                    <  1:360390/148‑1 (MQ=255)
                                                                                       cTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGc                                                                >  2:110833/1‑148 (MQ=255)
                                                                                        ttCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCGGAGGACGCACGCGACCGGGGCat                                                              >  4:310582/1‑148 (MQ=255)
                                                                                        ttCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACgggg                                                                 <  4:141570/146‑1 (MQ=255)
                                                                                        ttCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACgggg                                                                 >  3:141570/1‑146 (MQ=255)
                                                                                        ttCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGc                                                                >  3:93785/1‑147 (MQ=255)
                                                                                        ttCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGc                                                                >  2:64548/1‑147 (MQ=255)
                                                                                          catcaATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGa                                                            >  5:68021/1‑149 (MQ=255)
                                                                                            tcaatcaGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGAcc                                                          >  1:275341/1‑149 (MQ=255)
                                                                                               atcaGTACCCGAAAGTCAGCTACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGc                                                       <  4:344217/149‑1 (MQ=255)
                                                                                               atcaGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCg                                                                                                   <  1:138323/105‑1 (MQ=255)
                                                                                               atcaGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCg                                                                                                   >  2:138323/1‑105 (MQ=255)
                                                                                                   gTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGACCGGGGCAAGACCCGCTACg                                                   >  4:82884/1‑149 (MQ=255)
                                                                                                    tACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGt                                                  <  3:244318/149‑1 (MQ=255)
                                                                                                       ccGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGc                                                                                      <  8:226461/110‑1 (MQ=255)
                                                                                                       ccGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGc                                                                                      >  7:226461/1‑110 (MQ=255)
                                                                                                       ccGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTAtt                                               >  6:158753/1‑149 (MQ=255)
                                                                                                        cGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTg                                              <  2:275341/149‑1 (MQ=255)
                                                                                                                 gCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTAc                                                    <  7:129880/134‑1 (MQ=255)
                                                                                                                 gCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTAc                                                    >  8:129880/1‑134 (MQ=255)
                                                                                                                  ccACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGc                                    >  2:357082/1‑149 (MQ=255)
                                                                                                                   cacaGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGAAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCt                                   >  1:287013/1‑149 (MQ=255)
                                                                                                                           cATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAgggg                            <  4:76819/148‑1 (MQ=255)
                                                                                                                               cGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGgttgtt                       <  6:68021/149‑1 (MQ=255)
                                                                                                                                gTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGttgttg                      <  7:147197/149‑1 (MQ=255)
                                                                                                                                   gcgATATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGTTGGGCCGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGTTGTTGGCa                   <  1:147210/149‑1 (MQ=255)
                                                                                                                                   gcgAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCgtgg                                                                                                              <  7:291722/58‑1 (MQ=255)
                                                                                                                                   gcgAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCgtgg                                                                                                              >  8:291722/1‑58 (MQ=255)
                                                                                                                                         ccttGGCGCTCCAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGTTGTTGGCACCGACg             >  5:292699/4‑149 (MQ=255)
                                                                                                                                          cttGGCGCTCCAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGc                                    >  7:11611/3‑125 (MQ=255)
                                                                                                                                          cttGGCGCTCCAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGc                                    <  8:11611/123‑1 (MQ=255)
                                                                                                                                          aCTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGTTGTTGGCACCGACGt            >  7:280870/1‑149 (MQ=255)
                                                                                                                                          aCTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGTTGTTGGCACCGACGt            >  7:194667/1‑149 (MQ=255)
                                                                                                                                           cTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGTTGTTGGCACCGACGTg           <  4:84638/149‑1 (MQ=255)
                                                                                                                                                gcTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGTTGTTGGCACCGACGTGGCAgg      >  1:166798/1‑149 (MQ=255)
                                                                                                                                                    gAGCGCCAAGGGGTTGAGGTGCTACGTATAGCCCTGCTTGGGGGGGACTCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCGACGTATTGGAAGGTGGGCTCAAGGGGTGGTTGGCACCGCCTGGGCCGGTACt  >  6:171513/1‑149 (MQ=255)
                                                                                                                                                    gAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGTTGTTGGCACCGACGTGGCAGGTACt  <  1:357082/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCCGTAGTCGACAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGTTGTTGGCACCGACGTGGCAGGTACT  >  NC_002947/3554312‑3554608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑