Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,590,294 A→T 5.7% N272I (AAC→ATC)  nicP‑I → porin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,590,2940AT 5.7% 209.5 / 2.5 70N272I (AAC→ATC) nicP‑Iporin‑like protein
Reads supporting (aligned to +/- strand):  ref base A (29/37);  new base T (2/2);  total (31/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

GGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTT  >  NC_002947/3590150‑3590439
                                                                                                                                                |                                                                                                                                                 
ggTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAg                                                                                                                                               >  6:45783/1‑149 (MQ=255)
       tCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGttct                                                                                                                                        <  3:359275/149‑1 (MQ=255)
         aGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGttcttc                                                                                                                                      <  2:276147/149‑1 (MQ=255)
           cTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTtctc                                                                                                                                    <  1:269639/149‑1 (MQ=255)
              gaggaCATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCg                                                                                                                                   <  2:300310/147‑1 (MQ=255)
                   cATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCa                                                                                                                            <  6:315646/149‑1 (MQ=255)
                    aTCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCAc                                                                                                                           <  8:140389/149‑1 (MQ=255)
                       tACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAg                                                                                                                                               <  1:337901/126‑1 (MQ=255)
                       tACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAg                                                                                                                                               >  2:337901/1‑126 (MQ=255)
                          caacaaGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCg                                                                                                                     >  3:275976/1‑149 (MQ=255)
                            acaaGGCTTTGTTGGCCTGAAGCACATCAAACCCGTCGGCGACTGGGTACTCAGTTCCAAACCGGGATATTTCACCGCGAGTGAGCAAGGCGATGCGCTGCTGGTCAACATCGACAACCAGGCGTTCTTCTCGCTCTTCACTGCCCgg                                                                                                                    >  3:234592/1‑148 (MQ=255)
                            acaaGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGc                                                                                                                                  >  2:211321/1‑134 (MQ=255)
                            acaaGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGc                                                                                                                                  <  1:211321/134‑1 (MQ=255)
                            acaaGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGtt                                                                                                                              <  2:340896/138‑1 (MQ=255)
                            acaaGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGtt                                                                                                                              >  1:340896/1‑138 (MQ=255)
                             caaGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCa                                                                                                                  <  5:45783/149‑1 (MQ=255)
                               aGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATg                                                                                                                >  5:216523/1‑149 (MQ=255)
                                 gCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGc                                                                                                              <  8:32310/149‑1 (MQ=255)
                                     tgttgGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGAGGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCggg                                                                                                           <  4:39819/148‑1 (MQ=255)
                                       ttgGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATTCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGcac                                                                                                        <  2:14601/149‑1 (MQ=255)
                                            cTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCtt                                                                                                   >  6:323272/1‑149 (MQ=255)
                                                   acaGAAAACCCTTCGGCTACAGGGTAATCAGTTCCAACCTGTGTTATTTCACCTCGCTTGAGCAATTCGATGCGCTGCTGTGCAACATATACAACAAGGCGTTCTTCTCGCTGTTCAATGCCAGGAATGGCGGGACCAGCTACCATGcc                                                                                            >  6:361863/1‑149 (MQ=255)
                                                     aGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCgg                                                                                          >  5:80915/1‑149 (MQ=255)
                                                     aGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCgg                                                                                          >  4:144757/1‑149 (MQ=255)
                                                              gTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAggg                                                                                 <  6:216523/149‑1 (MQ=255)
                                                               tCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAggg                                                                                 >  4:259038/1‑148 (MQ=255)
                                                               tCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGc                                                                                <  6:80915/149‑1 (MQ=255)
                                                                cGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCa                                                                               >  8:308531/1‑149 (MQ=255)
                                                                 ggCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCa                                                                               <  5:323272/148‑1 (MQ=255)
                                                                  gCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCAtt                                                                             <  7:276768/149‑1 (MQ=255)
                                                                         ggTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACATCCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGc                                                                       >  8:80846/1‑148 (MQ=255)
                                                                         ggTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACATCCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGc                                                                       >  8:85898/1‑148 (MQ=255)
                                                                         ggTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGc                                                                       >  7:117588/1‑148 (MQ=255)
                                                                         ggTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCg                                                                      <  7:308531/149‑1 (MQ=255)
                                                                                 gTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCg                                                              >  5:251942/1‑149 (MQ=255)
                                                                                 gTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCg                                                              >  5:359607/1‑149 (MQ=255)
                                                                                   gCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGtt                                                            >  6:196578/1‑149 (MQ=255)
                                                                                     cAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTcc                                                          >  3:101297/1‑149 (MQ=255)
                                                                                     cAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTcc                                                          >  5:76505/1‑149 (MQ=255)
                                                                                         cTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACg                                                      <  5:361863/149‑1 (MQ=255)
                                                                                          tGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACgg                                                     >  8:2375/1‑149 (MQ=255)
                                                                                          tGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACgg                                                     >  3:228678/1‑149 (MQ=255)
                                                                                                  ttCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGcac                                                                                                        <  5:364668/90‑1 (MQ=255)
                                                                                                  ttCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGcac                                                                                                        >  6:364668/1‑90 (MQ=255)
                                                                                                     aCCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCaa                                          <  3:144757/149‑1 (MQ=255)
                                                                                                      ccGCGCGTGAGCAAGGCGATGCGCTCCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGATCCCACGGGTATTTGCCaa                                          >  2:259369/1‑148 (MQ=255)
                                                                                                       cgcgcgTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGtt                                                            >  7:307264/1‑129 (MQ=255)
                                                                                                       cgcgcgTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGtt                                                            <  8:307264/129‑1 (MQ=255)
                                                                                                          gcgTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACAtct                                     >  1:148862/1‑149 (MQ=255)
                                                                                                            gTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATgg                                                                        <  4:118653/112‑1 (MQ=255)
                                                                                                            gTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATgg                                                                        >  3:118653/1‑112 (MQ=255)
                                                                                                                 cAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGtt                              <  4:275976/149‑1 (MQ=255)
                                                                                                                  aaGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCg                                   <  2:332104/143‑1 (MQ=255)
                                                                                                                  aaGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCg                                   >  1:332104/1‑143 (MQ=255)
                                                                                                                  aaGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTg                             <  7:2375/149‑1 (MQ=255)
                                                                                                                   aGGCGATGCGCTGCTGGGCAACATCGACATCCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTgg                            <  7:80846/149‑1 (MQ=255)
                                                                                                                   aGGCGATGCGCTGCTGGGCAACATCGACATCCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTgg                            <  7:85898/149‑1 (MQ=255)
                                                                                                                   aGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTgg                            <  4:234592/149‑1 (MQ=255)
                                                                                                                   aGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTgg                            <  8:117588/149‑1 (MQ=255)
                                                                                                                        aTGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAAc                       <  3:259038/149‑1 (MQ=255)
                                                                                                                           cgcTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGa                     <  4:101297/148‑1 (MQ=255)
                                                                                                                              tgcttGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCAc                                                       <  6:335072/111‑1 (MQ=255)
                                                                                                                                   ggCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGAc            <  5:196578/149‑1 (MQ=255)
                                                                                                                                    gCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGtt                                                            >  3:33317/1‑100 (MQ=255)
                                                                                                                                    gCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGtt                                                            <  4:33317/100‑1 (MQ=255)
                                                                                                                                      aaCATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTa         >  8:264637/1‑149 (MQ=255)
                                                                                                                                       aCATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTAt        <  3:189631/149‑1 (MQ=255)
                                                                                                                                         aTCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGa      <  6:359607/149‑1 (MQ=255)
                                                                                                                                         aTCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGa      <  4:228678/149‑1 (MQ=255)
                                                                                                                                             aCAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGttt  <  6:76505/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GGTACGGTCAGCTGGAGGACATCTACCAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTT  >  NC_002947/3590150‑3590439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑