Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,659,906 C→G 7.4% intergenic (‑41/+34) PP_3223 ← / ← PP_3224 ABC transporter substrate‑binding protein/class II aldolase/adducin domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,659,9060CG 7.4% 164.1 / 3.4 54intergenic (‑41/+34)PP_3223/PP_3224ABC transporter substrate‑binding protein/class II aldolase/adducin domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (26/24);  new base G (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01

GGCGATGTTGAGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCACCT  >  NC_002947/3659761‑3660052
                                                                                                                                                 |                                                                                                                                                  
ggCGATGTTGAGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGt                                                                                                                                                 >  4:17871/1‑149 (MQ=255)
          aGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAAc                                                                                                                                       <  7:254726/149‑1 (MQ=255)
          aGGGGCTTGCTGGTTGAGGGGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCGAACTGGCCGGaa                                                                                                                                        <  2:119972/148‑1 (MQ=255)
            ggTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGGGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGGGCAGTGGGGGCATGGGATTGCTCCTTCAATGAAGGGGGCTCTCCTGTTCCGGCGACCTGGCCGGAACAg                                                                                                                                     <  1:83163/149‑1 (MQ=255)
             gTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAgg                                                                                                                                    <  2:155057/149‑1 (MQ=255)
              tCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGc                                                                                                                                   <  7:102481/149‑1 (MQ=255)
                      gTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTg                                                                                                                           >  2:91876/1‑149 (MQ=255)
                       ttGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGc                                                                                                                          <  7:261670/149‑1 (MQ=255)
                        tGAGGTCTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGACTGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGcc                                                                                                                         >  8:5214/1‑149 (MQ=255)
                           ggTGTTGTCGGCCGGCGAGGAAGCGGTGAGGCGCAGGGCCATGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCtt                                                                                                                       <  5:18289/148‑1 (MQ=255)
                            gtgtTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAATGTGCCTTg                                                                                                                      <  1:367464/148‑1 (MQ=255)
                                 gccggccggcGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGagt                                                                                                                  >  3:288418/3‑147 (MQ=255)
                                  tCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGagtcag                                                                                                               >  6:98622/1‑149 (MQ=255)
                                  tCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGagtca                                                                                                                <  2:103026/148‑1 (MQ=255)
                                                aGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAg                                                                                                 >  1:48518/1‑149 (MQ=255)
                                                aGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAg                                                                                                 <  3:26437/149‑1 (MQ=255)
                                                 gCGGTGCGGCCCAGGGCCAGGGGGCCGATCGCGAGCAGTTGGTGCAGTCGGGGCATGGGATTGATCCTTCAATGAATCGGGCTCTCCTGTTCCGGCCAGCTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAgg                                                                                                <  4:288418/149‑1 (MQ=255)
                                                          cccAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGa                                                                                       >  2:74831/1‑149 (MQ=255)
                                                           ccagggccagggTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGAt                                                                                      >  1:12382/1‑149 (MQ=255)
                                                            cagggccagggTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATc                                                                                     <  4:306205/149‑1 (MQ=255)
                                                                          cGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAAt                                                                         >  5:63273/1‑147 (MQ=255)
                                                                          cGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAAt                                                                         >  4:361889/1‑147 (MQ=255)
                                                                            aTCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAgc                                                                     >  5:191565/1‑149 (MQ=255)
                                                                                                   gggCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATAcc                                              <  1:91876/149‑1 (MQ=255)
                                                                                                     gCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCtt                                            <  2:48518/149‑1 (MQ=255)
                                                                                                           taTTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTACTGGTAATCGAGTCCCTCTCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCt                                      >  5:308888/2‑149 (MQ=255)
                                                                                                           gATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTc                                                                                           >  5:377972/1‑96 (MQ=255)
                                                                                                           gATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTc                                                                                           <  6:377972/96‑1 (MQ=255)
                                                                                                            aTTGATCATTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTg                                     >  3:131313/1‑149 (MQ=255)
                                                                                                                aTCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAAt                                                           <  1:335189/123‑1 (MQ=255)
                                                                                                                aTCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAAt                                                           >  2:335189/1‑123 (MQ=255)
                                                                                                                aTCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTg                                                    >  1:54459/1‑130 (MQ=255)
                                                                                                                aTCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTg                                                    <  2:54459/130‑1 (MQ=255)
                                                                                                                 tCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCAt                                >  3:358007/1‑149 (MQ=255)
                                                                                                                   cTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCg                              <  3:17871/149‑1 (MQ=255)
                                                                                                                   cTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCg                              <  1:74831/149‑1 (MQ=255)
                                                                                                                    ttCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGACGTTGGATACATTCCCCCTGGCCATCg                              >  5:242536/1‑148 (MQ=255)
                                                                                                                      cAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGa                            >  6:302442/1‑148 (MQ=255)
                                                                                                                       aatgaatgGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGaa                           >  8:116745/1‑148 (MQ=255)
                                                                                                                       aatgaatgGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGaa                           >  3:46877/1‑148 (MQ=255)
                                                                                                                          gaatgGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAAcc                       >  8:278857/1‑149 (MQ=255)
                                                                                                                           aatgGGGCTCTCCTGTTCCGGACAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAAcc                       <  4:131313/148‑1 (MQ=255)
                                                                                                                               gggCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTTCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCTCTTg                  <  4:358007/149‑1 (MQ=255)
                                                                                                                               gggCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCtt                   <  5:98622/148‑1 (MQ=255)
                                                                                                                                 gCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGa                <  2:12382/149‑1 (MQ=255)
                                                                                                                                   tctcCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATc                               >  4:173284/1‑132 (MQ=255)
                                                                                                                                   tctcCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATc                               <  3:173284/132‑1 (MQ=255)
                                                                                                                                      ccTGTTCCGGCGAACTGGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGc            >  6:358864/1‑148 (MQ=255)
                                                                                                                                      ccTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCt           >  5:46203/1‑149 (MQ=255)
                                                                                                                                       cTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTc          <  7:116745/149‑1 (MQ=255)
                                                                                                                                         gTTCCGGCGAACTGGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCAt        >  1:349060/1‑149 (MQ=255)
                                                                                                                                              ggCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCtt                   <  1:366684/133‑1 (MQ=255)
                                                                                                                                              ggCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCtt                   >  2:366684/1‑133 (MQ=255)
                                                                                                                                               gCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCACCt  >  4:305181/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GGCGATGTTGAGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCACCT  >  NC_002947/3659761‑3660052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑