Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 274,791 C→A 8.7% G386V (GGC→GTC)  PP_0222 ← DszA family monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947274,7910CA 8.7% 165.1 / 4.5 56G386V (GGC→GTC) PP_0222DszA family monooxygenase
Reads supporting (aligned to +/- strand):  ref base C (29/21);  new base A (3/2);  total (33/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

GCCTGGAACAGTTTTTCGCGCAGGGTGCCTTCGGTGTAGGCAGTCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCT  >  NC_002947/274647‑274931
                                                                                                                                                |                                                                                                                                            
gCCTGGAACAGTTTTTCGCGCAGGGTGCCTTCGGTGTAGGCAGTCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGTGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGt                                                                                                                                          <  7:344917/149‑1 (MQ=255)
   tGGAACAGTTTTTCGCGCAGGGTGCCTTCGGTGTAGGCAGTCTTGTAGCGGCCACGCTGCTGAAGTTGCTGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGc                                                                                                                                              <  8:25945/142‑1 (MQ=255)
   tGGAACAGTTTTTCGCGCAGGGTGCCTTCGGTGTAGGCAGTCTTGTAGAGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGc                                                                                                                                              >  7:25945/1‑142 (MQ=255)
     gAACAGTTTTTCGCGCAGGGTGCCTTCGGTGTAGGCAGTCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCAt                                                                                                                                     >  5:67273/1‑149 (MQ=255)
     gAACAGTTTTTCGCGCAGGGTGCCTTCGGTGTAGGCAGTCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCAt                                                                                                                                     >  5:307341/1‑149 (MQ=255)
      aaCAGTTTTTCGCGCAGGGTGCCTTCGGTGTAGGCAGTCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATc                                                                                                                                    <  7:94321/149‑1 (MQ=255)
          gTTTTTCGCGCAGGGTGCCTTCGGTGTAGGCAGTCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATc                                                                                                                                >  1:24344/1‑149 (MQ=255)
           tttttCGCGCAGGGTGCCTTCGGTGTAGGCAGTCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCAt                                                                                                                                     <  6:26017/143‑1 (MQ=255)
           tttttCGCGCAGGGTGCCTTCGGTGTAGGCAGTCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCAt                                                                                                                                     >  5:26017/1‑143 (MQ=255)
           tttttCGCGCAGGGTGCCTTCGGTGTAGGCAGTCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATc                                                                                                                                >  3:42798/1‑148 (MQ=255)
                     aGGGCGCCTTCGGTGTAGGCAGTCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCAGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCaa                                                                                                                     <  5:207753/149‑1 (MQ=255)
                             ttCGGTGTAGGCAGTCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCgg                                                                                                             >  3:162998/1‑149 (MQ=255)
                             ttCGGTGTAGGCAGTCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCgg                                                                                                             >  3:168576/1‑149 (MQ=255)
                             ttCGGTGTAGGCAGTCTTGTAGCGGCCACGCTGCTGAAGATGCGCGATGACCAGCTCGATGAAGTCCTCGAAGATCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCATGCCGGTTTCATCGATACAAGCGATCAACTCATCgg                                                                                                             >  7:251540/1‑149 (MQ=255)
                               caccgaaGGCAGTCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGc                                                                                                            <  3:277136/142‑1 (MQ=255)
                                ggTGTAGGCAGTCTTGTAGCGGCCACGCTGCTGAAGTTGTGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCa                                                                                                          <  2:218928/149‑1 (MQ=255)
                                    tAGGCAGTCTTGTAGCGTCCACGCTGCTGAAGTTGCGGGATGACCATCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAAGAGTCCAGGACGGTTTCATGGATACACGCGATCAACTGCTCGGCCACCt                                                                                                       <  8:251540/148‑1 (MQ=255)
                                    tAGGCAGTCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTg                                                                                                      >  2:326835/1‑149 (MQ=255)
                                        cAGTCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGtt                                                                                                    >  6:238754/1‑147 (MQ=255)
                                        cAGTCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGtt                                                                                                    <  5:238754/147‑1 (MQ=255)
                                           tCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGgc                                                                                               >  4:363613/1‑149 (MQ=255)
                                           tCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGgc                                                                                               <  1:204888/149‑1 (MQ=255)
                                                tAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCg                                                                                                                                            >  2:285179/1‑99 (MQ=255)
                                                tAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCg                                                                                                                                            <  1:285179/99‑1 (MQ=255)
                                                              ctgAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGCTCAGGCGCGCCGACCAGGGTTAc                                                                              <  4:42798/147‑1 (MQ=255)
                                                               tgAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACg                                                                             >  7:229844/1‑147 (MQ=255)
                                                                    ttGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGgc                                                                      <  3:363613/149‑1 (MQ=255)
                                                                         ggATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGgccgcc                                                                  >  5:238379/1‑148 (MQ=255)
                                                                                      gATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGtt                                                    >  8:102850/1‑149 (MQ=255)
                                                                                         gAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGc                                                  >  4:112252/1‑148 (MQ=255)
                                                                                         gAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGc                                                  >  7:335726/1‑148 (MQ=255)
                                                                                             tCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTAc                                                                              <  1:320206/116‑1 (MQ=255)
                                                                                             tCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTAc                                                                              >  2:320206/1‑116 (MQ=255)
                                                                                               cTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGgc                                           >  5:84900/1‑149 (MQ=255)
                                                                                               cTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGgc                                           >  6:284797/1‑149 (MQ=255)
                                                                                               cTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGAACCACGCGATAAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAACGCGTGCTGTTGCAACAGgc                                           >  5:61966/1‑149 (MQ=255)
                                                                                                       tctcCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCcggcgg                                   >  1:20800/1‑149 (MQ=255)
                                                                                                            ggggTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGCCGTTCTGTTGCAACAGCCGCCCGCGGGTc                                >  7:270184/1‑147 (MQ=255)
                                                                                                                  aCGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGt                         <  6:67273/148‑1 (MQ=255)
                                                                                                                        cGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCtt                   <  8:229844/148‑1 (MQ=255)
                                                                                                                         gggTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTg                       <  4:244966/143‑1 (MQ=255)
                                                                                                                         gggTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTg                       >  3:244966/1‑143 (MQ=255)
                                                                                                                         gggTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGc                 <  2:24344/149‑1 (MQ=255)
                                                                                                                          ggTCAGGTTGAAACCGTCCAGGTCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCCGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGc                 >  7:56529/1‑148 (MQ=255)
                                                                                                                           gTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCaa               <  3:112252/149‑1 (MQ=255)
                                                                                                                              tcgatGAAACCGGCCTGGACGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCCACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTg            >  5:374082/5‑149 (MQ=255)
                                                                                                                              aGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTg            <  6:238379/149‑1 (MQ=255)
                                                                                                                                gTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGgc          >  8:295925/1‑149 (MQ=255)
                                                                                                                                 atGAAACCGGCCTGGACGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGc                           >  8:235363/2‑131 (MQ=255)
                                                                                                                                 atGAAACCGGCCTGGACGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGc                           <  7:235363/130‑1 (MQ=255)
                                                                                                                                 atGAAACCGGCCTGGACGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGgcg         >  6:68104/2‑149 (MQ=255)
                                                                                                                                     aaCCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCt                                                       <  4:101369/99‑1 (MQ=255)
                                                                                                                                     aaCCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCt                                                       >  3:101369/1‑99 (MQ=255)
                                                                                                                                      aCCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTgg    >  8:242248/1‑149 (MQ=255)
                                                                                                                                       ccGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGc   <  7:295925/149‑1 (MQ=255)
                                                                                                                                         gTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCTGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCt  >  8:138206/1‑148 (MQ=255)
                                                                                                                                          tCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCcggc                                     <  3:249764/112‑1 (MQ=255)
                                                                                                                                          tCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCcggc                                     >  4:249764/1‑112 (MQ=255)
                                                                                                                                                |                                                                                                                                            
GCCTGGAACAGTTTTTCGCGCAGGGTGCCTTCGGTGTAGGCAGTCTTGTAGCGGCCACGCTGCTGAAGTTGCGGGATGACCAGCTCGATGAAGTCCTCGAAGCTCTCCGGGGTGACGGTGCGGGTCAGGTTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCT  >  NC_002947/274647‑274931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑