Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,697,084	C→G	6.3%	W135C (TGG→TGC)	PP_5595 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,697,084	0	C	G	6.3%	192.2 / 4.1	64	W135C (TGG→TGC)	PP_5595	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (28/31);  new base G (2/2);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

TGGATGAAACCTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCC‑AATGCCTCGGG  >  NC_002947/3696940‑3697224                                                                                                                                                 |                                                                                                                                                tGGATGAAACCTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGa                                                                                                                                             >  1:117378/1‑149 (MQ=255)     tGAAACCTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGAcccc                                                                                                                                         <  1:275674/149‑1 (MQ=255)     tGAAACCTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGAcccc                                                                                                                                         >  7:322939/1‑149 (MQ=255)        aaCCTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGGCAGAcccacta                                                                                                                                      <  7:301845/149‑5 (MQ=255)        aaCCTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGGCAGAcccac                                                                                                                                        >  8:301845/1‑145 (MQ=255)         aCCTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑a                                                                                                                                    >  8:160253/1‑149 (MQ=255)          ccTTCCCAGCGCGGGATCCGATCTCGCCCTGAAGCCGCAGAGGCGGGCGGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCGCCCAGCCGTTTGCCCTAGGGGGGCTGCCAGACCCCGTT‑At                                                                                                                                   <  6:219127/149‑1 (MQ=255)           cTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCGAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑Att                                                                                                                                  <  8:322939/149‑1 (MQ=255)               ccAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCg                                                                                                                               <  2:225035/148‑1 (MQ=255)               ccAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGc                                                                                                                              >  6:219551/1‑149 (MQ=255)                 aGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCt                                                                                                                             >  4:94356/1‑148 (MQ=255)                       tGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTc                                                                                                                      <  5:9635/149‑1 (MQ=255)                          tCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGGCAGAcccacta                                                                                                                                      >  7:182049/1‑127 (MQ=255)                          tCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGGCAGAcccacta                                                                                                                                      <  8:182049/131‑5 (MQ=255)                                    cccTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTgg                                                                                                         <  2:117378/149‑1 (MQ=255)                                      cTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGcc                                                                                                       >  2:105576/1‑149 (MQ=255)                                         aaGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGa                                                                                                     <  1:177799/148‑1 (MQ=255)                                         aaGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGa                                                                                                     <  3:125048/148‑1 (MQ=255)                                         aaGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCATTTTGCCCTAGTGGGTCTGACAGCCCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCGGAg                                                                                                    >  6:288300/1‑149 (MQ=255)                                               aaTAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCg                                                                                                                               >  3:133623/1‑116 (MQ=255)                                               aaTAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCg                                                                                                                               <  4:133623/116‑1 (MQ=255)                                                 tAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACGCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCgggg                                                                                           >  2:136837/1‑149 (MQ=255)                                                  aGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCgggg                                                                                           >  3:222331/1‑148 (MQ=255)                                                    gCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTGTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACGACGGGCCTGGCCGAGGC‑GCGGGGTag                                                                                        >  6:85598/1‑148 (MQ=255)                                                     cGGGCTGCCCGTGCAGGCGATCGCCCCGCCCTTTGATCTACTGCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGGCTGCCAGCCCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATc                                                                                       <  5:85598/149‑1 (MQ=255)                                                       ggCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGACCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGt                                                                                     <  6:343788/149‑1 (MQ=255)                                                               ttGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGt                                                                               >  2:172280/1‑147 (MQ=255)                                                                tGCAGGCGATCGCCCCGCCCTTTGATCTGATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAg                                                                            >  3:298072/1‑149 (MQ=255)                                                                 tctggCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACTGTGACTAGGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTat                                                                             <  5:288300/144‑2 (MQ=255)                                                                 gCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTaa                                                                             <  5:219551/147‑1 (MQ=255)                                                                  cAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGt                                                                           <  8:54055/148‑1 (MQ=255)                                                                  cAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGt                                                                           <  2:290639/148‑1 (MQ=255)                                                                   aGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGa                                                                         <  7:160253/149‑1 (MQ=255)                                                                   aGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGa                                                                         <  1:172280/149‑1 (MQ=255)                                                                           gccccgcccTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCcgc                                                                 <  1:83843/149‑1 (MQ=255)                                                                            ccccgcccTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCcgcg                                                                >  8:225539/1‑149 (MQ=255)                                                                             cccgcccTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATa                                                                                 <  8:136519/131‑1 (MQ=255)                                                                             cccgcccTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATa                                                                                 >  7:136519/1‑131 (MQ=255)                                                                             cccgcccTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCcgcg                                                                >  6:104237/1‑148 (MQ=255)                                                                                    tttGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTg                                                        >  6:300440/1‑149 (MQ=255)                                                                                       gATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTggg                                                      <  3:94356/148‑1 (MQ=255)                                                                                         tCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCaa                                                   >  1:296948/1‑149 (MQ=255)                                                                                              ttCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCg                                              >  3:349101/1‑149 (MQ=255)                                                                                               tCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCgg                                             >  5:290111/1‑149 (MQ=255)                                                                                                        gggCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGc                                     <  1:105576/149‑1 (MQ=255)                                                                                                          gCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCAc                                  <  4:298072/149‑1 (MQ=255)                                                                                                          gCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCAc                                  >  4:26848/1‑149 (MQ=255)                                                                                                          gCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCGCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCAc                                  >  5:95710/1‑149 (MQ=255)                                                                                                              gcgcTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTAt                              >  2:10889/1‑149 (MQ=255)                                                                                                                      tCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTgg                      >  5:96518/1‑149 (MQ=255)                                                                                                                        ccAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGa                    >  1:284570/1‑149 (MQ=255)                                                                                                                        ccAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCGCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGa                    <  6:95710/149‑1 (MQ=255)                                                                                                                         cAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTgg                                                       >  7:19433/1‑113 (MQ=255)                                                                                                                         cAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTgg                                                       <  8:19433/113‑1 (MQ=255)                                                                                                                             cTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGc               >  8:330162/1‑149 (MQ=255)                                                                                                                             cTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGc               <  2:296948/149‑1 (MQ=255)                                                                                                                                ttGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGcc              <  7:225539/147‑1 (MQ=255)                                                                                                                                  gCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCC‑AAt          <  7:330162/148‑1 (MQ=255)                                                                                                                                    ccTAGTGGGTCTGCCAGACCCCGTTAATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGAtt                  >  1:192226/1‑140 (MQ=255)                                                                                                                                    ccTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGAtt                  <  2:192226/139‑1 (MQ=255)                                                                                                                                    ccTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGCCTTCTCGCCACCTATTGCCTTGGGATTGGCC‑AATGcc       >  7:132002/1‑149 (MQ=255)                                                                                                                                        gTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCC‑AATGCCTCgg   <  1:10889/149‑1 (MQ=255)                                                                                                                                         tGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑GCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCC‑AATGCCTCggg  <  2:285877/149‑1 (MQ=255)                                                                                                                                           ggTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCCAAATGCCTCggg  <  6:290111/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                TGGATGAAACCTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTT‑ATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGC‑TCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCC‑AATGCCTCGGG  >  NC_002947/3696940‑3697224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑