Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,757,356 A→G 5.8% T60A (ACC→GCC)  PP_3321 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,757,3560AG 5.8% 198.7 / 2.3 69T60A (ACC→GCC) PP_3321hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (32/33);  new base G (2/2);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-01

GCCGCTACTGTTCATCTTTTCCACTTGCGCAGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAACTATTTCGAGAATTTCCTCGCTAACGACGGGCTGGT  >  NC_002947/3757215‑3757501
                                                                                                                                             |                                                                                                                                                 
gccgcTACTGTTCATCTTTTCCACTTGCGCAGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGc                                                                                                                                            <  3:164706/149‑1 (MQ=255)
 ccgcTACTGTTCATCTTTTCCACTTGCGCAGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGcc                                                                                                                                           <  2:165522/149‑1 (MQ=255)
   gcTACTGTTCATCTTTTCCACTTGCGCAGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCca                                                                                                                                          >  7:145926/1‑148 (MQ=255)
   gcTACTGTTCATCTTTTCCACTTGCGCACGAGAGACGCCATTTTATTTTTATTTGATAAGTCGACCATCGATATCTACTCCGACACCGAACTGGCCTGCCTTGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCcac                                                                                                                                         <  4:158994/149‑1 (MQ=255)
    cTACTGTTCATCTTTACCACTTGCGCAGGAGAGACGCCATGACCGAAACAAGCCAGAACCCGACCATCGAACCCTCCGCCGACACCGAACAGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGGTGGGCCGCGACCGTGGCcaca                                                                                                                                        <  5:285173/149‑1 (MQ=255)
       cTGTTCATCTTTTCCACTTGCGCAGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCcac                                                                                                                                         <  1:367057/145‑1 (MQ=255)
       cTGTTCATCTTTTCCACTTGCGCAGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTCGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCcac                                                                                                                                         >  2:367057/1‑145 (MQ=255)
          ttCATCTTTTCCACTTGCGCCGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGAGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGAt                                                                                                                                  <  5:277435/149‑1 (MQ=255)
          ttCATCTTTTCCACTTGCGCAGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGAt                                                                                                                                  >  5:576/1‑149 (MQ=255)
            cATCTTTTCCACTTGCGCAGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGAtgt                                                                                                                                >  2:118113/1‑149 (MQ=255)
             aTCTTTTCCACTTGCGCAGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGAtgtg                                                                                                                               >  7:283369/1‑149 (MQ=255)
             aTCTTTTCCACTTGCGCAGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGAtgt                                                                                                                                <  7:174917/148‑1 (MQ=255)
             aTCTTTTCCACTTGCGCAGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGAtgt                                                                                                                                <  6:171996/148‑1 (MQ=255)
             aTCTTTACCACTTGCTCATGAGAGTCGCAATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGAtgt                                                                                                                                <  4:39593/148‑1 (MQ=255)
                ttttCCACTTGCGCAGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGAt                                                                                                                            >  8:235564/1‑149 (MQ=255)
                  ttCCACTTGCGCAGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAACCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTgg                                                                                                                              <  1:262951/145‑1 (MQ=255)
                      aCTTGCGCAGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTgg                                                                                                                              <  6:184053/141‑1 (MQ=255)
                      aCTTGCGCAGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTgg                                                                                                                              >  5:184053/1‑141 (MQ=255)
                           cgcAGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGc                                                                                                                 >  4:287145/1‑149 (MQ=255)
                              aGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGtt                                                                                                              <  8:145926/149‑1 (MQ=255)
                              aGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGtt                                                                                                              <  3:287145/149‑1 (MQ=255)
                                   agaCGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACg                                                                                                         >  3:339536/1‑149 (MQ=255)
                                     aCGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCa                                                                                                       >  8:127379/1‑149 (MQ=255)
                                            gACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATg                                                                                                >  4:15095/1‑149 (MQ=255)
                                               cGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGa                                                                                             >  6:359503/1‑149 (MQ=255)
                                                gAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGAt                                                                                            >  7:119704/1‑149 (MQ=255)
                                                 aaaCATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATc                                                                                           <  3:147812/149‑1 (MQ=255)
                                                         cAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTcc                                                                                   <  8:119704/149‑1 (MQ=255)
                                                         cAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCACGGTCGCGGcccaac                                                                                                                                       <  7:302943/97‑5 (MQ=255)
                                                         cAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCACGGTCGCGGcccaac                                                                                                                                       >  8:302943/1‑93 (MQ=255)
                                                           gAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGa                                                                                 >  5:350679/1‑149 (MQ=255)
                                                            aaCCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGAc                                                                                <  7:127379/149‑1 (MQ=255)
                                                               ccGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCAcc                                                                                                     >  7:44350/1‑125 (MQ=255)
                                                               ccGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCAcc                                                                                                     <  8:44350/125‑1 (MQ=255)
                                                                 gACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGt                                                                           <  4:92104/149‑1 (MQ=255)
                                                                  aCCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGAACCTGATGCGATCCTGGTTCCGACCCTGTc                                                                          <  1:32082/149‑1 (MQ=255)
                                                                        gAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTccaacc                                                                     <  7:235564/148‑1 (MQ=255)
                                                                             cTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGc                                                               <  5:55024/149‑1 (MQ=255)
                                                                                cGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGt                                                             <  3:213734/148‑1 (MQ=255)
                                                                                cGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGt                                                             <  1:118113/148‑1 (MQ=255)
                                                                                cGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGt                                                             <  5:359503/148‑1 (MQ=255)
                                                                                  ccGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGAc                                                          >  5:163399/1‑149 (MQ=255)
                                                                                    gACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGGCCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTg                                                        >  7:109367/1‑149 (MQ=255)
                                                                                        ccGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGcgac                                                    <  1:161352/149‑1 (MQ=255)
                                                                                         cGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGcgac                                                    >  7:183738/1‑148 (MQ=255)
                                                                                           aaCTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGcgacga                                                  >  1:155811/1‑148 (MQ=255)
                                                                                            aCTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACa                                                <  6:350679/149‑1 (MQ=255)
                                                                                             cTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAg                                               >  2:307280/1‑149 (MQ=255)
                                                                                                gcctgcctGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGc                                              <  6:576/147‑1 (MQ=255)
                                                                                                     cctgGCAGGTGCCAAAGCGGCGCTCTAACATTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGc                                        >  1:204766/1‑148 (MQ=255)
                                                                                                        ggCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGa                                                                                 >  6:16423/1‑104 (MQ=255)
                                                                                                        ggCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGa                                                                                 <  5:16423/104‑1 (MQ=255)
                                                                                                         gCAGGTGCCAAAGCTGCGCTCTACCGTTGGGCCGCGACCGTGGCCAGACGTGATGTGGATCAGGTGCTGGCGTTGTACGCAACTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAGCTa                                   >  8:166935/1‑149 (MQ=255)
                                                                                                         gCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAACTa                                   >  1:325057/1‑149 (MQ=255)
                                                                                                                ccAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAACTATTTCga                             <  4:339536/148‑1 (MQ=255)
                                                                                                                 cAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAACTATTTCgag                            >  1:245363/1‑148 (MQ=255)
                                                                                                                  aaaGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAACTATTTCGAGaa                          >  8:148170/1‑149 (MQ=255)
                                                                                                                   aaGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAACTATTTCga                             <  2:349094/145‑1 (MQ=255)
                                                                                                                   aaGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAACTATTTCga                             >  1:349094/1‑145 (MQ=255)
                                                                                                                   aaGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAACTATTTCGAGAAt                         >  2:69821/1‑149 (MQ=255)
                                                                                                                        gcgcTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAACTATTTCGAGAATTTCCt                    >  8:70342/1‑149 (MQ=255)
                                                                                                                             ctACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAACTATTTCGAGAATTTCCTCGCt                >  8:78935/1‑148 (MQ=255)
                                                                                                                               aCCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAACTATTTCGAGAATTTCCTCGCTAac             >  3:265139/1‑149 (MQ=255)
                                                                                                                               aCCGTTGGGCCGCGACCGTGGCCACACGAGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAACTATTTCGAGAATTTCCTCGCTAac             >  1:22905/1‑149 (MQ=255)
                                                                                                                                  gTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAACTATTTCGAGAATTTCCTCGCTAacgac          <  2:155811/149‑1 (MQ=255)
                                                                                                                                  gTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAACTATTTCGAGAATTTCCTCGCTAacgac          >  4:34831/1‑149 (MQ=255)
                                                                                                                                   ttGGGCCGCGGCCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAACTATTTCGAGAATTTCCTCGCTAacgacg         <  8:109367/149‑1 (MQ=255)
                                                                                                                                          gcgACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAACTATTTCGAGAATTTCCTCGCTAACGACGGGCTGGt  <  1:307280/149‑1 (MQ=255)
                                                                                                                                          gcgACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAACTATTTCGAGAATTTCCTCGCTAACGACGGGCTGGt  <  6:163399/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
GCCGCTACTGTTCATCTTTTCCACTTGCGCAGGAGAGACGCCATGACCGAAACATGCCAGAACCCGACCATCGAAACCTACGCCGACACCGAACTGGCCTGCCTGGCAGGTGCCAAAGCGGCGCTCTACCGTTGGGCCGCGACCGTGGCCACACGTGATGTGGATCAGGTGCTGGCGTTGTACGCACCTGATGCGATCCTGGTTCCGACCCTGTCCAACCAAGTGCGTGACTGCGACGACAGCCGTCGCAACTATTTCGAGAATTTCCTCGCTAACGACGGGCTGGT  >  NC_002947/3757215‑3757501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑