Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,762,865	G→A	5.5%	V36V (GTG→GTA)	PP_3326 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,762,865	0	G	A	5.5%	279.6 / 3.5	90	V36V (GTG→GTA)	PP_3326	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (48/37);  new base A (3/2);  total (51/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.13e-01

CTGACCCTGAGCCTTTGAACCGAGGAACCATCATGCATCCATCGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGC  >  NC_002947/3762726‑3763008                                                                                                                                             |                                                                                                                                                 cTGACCCTGAGCCTTTGAACCGAGGAACCATCATGCATCCATCGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCAc                                                                                                                                         >  5:190100/1‑149 (MQ=255) cTGACCCTGAGCCTTTGAACCGAGGAACCATCATGCACCCATCGCTGAAAGTCGCTTCGCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTACACGGcctc                                                                                                                                         <  6:33881/149‑3 (MQ=255)    aCCCTGAGCCTTTGAACCGAGGAACGATCATGCATCCATCGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACg                                                                                                                                        <  8:314938/147‑1 (MQ=255)      ccTGAGCCTTTGAACCGAGGAACCATCATGCATCCATCGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGgcgc                                                                                                                                    >  8:242453/1‑149 (MQ=255)      ccTGAGCCTTTGAACCGAGGAACCATCATGCATCCATCGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGgcgc                                                                                                                                    >  7:43641/1‑149 (MQ=255)      ccTGAGCCTTTGAACCGAGGAACCATCATGCATCCATCGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGgcgc                                                                                                                                    >  7:179294/1‑149 (MQ=255)              tttGAACCGAGGAACCATCATGCATCCATCGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGAt                                                                                                                             >  6:115211/1‑148 (MQ=255)                 gAACCGAGGAACCATCATGCATCCATCGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGGCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAAc                                                                                                                          <  5:198133/148‑1 (MQ=255)                   aCCGAGGAACCATCATGCATCCATCGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGc                                                                                                                        <  8:201410/148‑1 (MQ=255)                    ccGAGGAACCATCATGCATCCATCGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGcc                                                                                                                       >  6:123664/1‑148 (MQ=255)                          aaCCATCATGCATCCATCGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAgg                                                                                                                <  4:334029/149‑1 (MQ=255)                           aCCATCATGCATCCATCGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGc                                                                                                               <  1:249887/149‑1 (MQ=255)                               tcatGCATCCATCGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGa                                                                                                            >  8:63388/1‑148 (MQ=255)                                  tGCATCCATCGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCTAGGATAACGCCTACAAGGCGGAAAAg                                                                                                        <  1:11296/149‑1 (MQ=255)                                  tGCATCCATCGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGaa                                                                                                           >  6:18688/1‑146 (MQ=255)                                    catccatcGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCATCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CTGAGGAGCGACGTGGCAATATCGAGGCCGTGTAGGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGAt                                                                                                      <  4:31666/149‑1 (MQ=255)                                    catccatcGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGa                                                                                                       <  5:115211/148‑1 (MQ=255)                                    catccatcGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGAt                                                                                                      <  1:134990/149‑1 (MQ=255)                                        catcGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAg                                                                                                  >  7:9053/1‑149 (MQ=255)                                         atcGCTGAAAGTCGATTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAgc                                                                                                 >  5:138655/1‑149 (MQ=255)                                           cGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAgcgc                                                                                               >  2:234360/1‑149 (MQ=255)                                             cTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAgcgcgc                                                                                             >  7:49638/1‑149 (MQ=255)                                              tGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACaa                                                                                                                  >  6:144471/1‑127 (MQ=255)                                              tGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACaa                                                                                                                  <  5:144471/127‑1 (MQ=255)                                               gAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAgc                                                                                                 >  3:53781/1‑143 (MQ=255)                                               gAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAgc                                                                                                 <  4:53781/143‑1 (MQ=255)                                                aaaGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTg                                                                                          >  2:24966/1‑149 (MQ=255)                                                      gCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGt‑t                                                                                   >  3:243625/1‑149 (MQ=255)                                                      gCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGt‑t                                                                                   >  4:354585/1‑149 (MQ=255)                                                        ttCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGATCCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑Tc                                                                                  >  1:281593/1‑149 (MQ=255)                                                        ttCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑Tc                                                                                  >  5:156814/1‑148 (MQ=255)                                                          cccTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGa                                                                                <  1:234360/148‑1 (MQ=255)                                                           ccTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGAc                                                                               <  3:354585/148‑1 (MQ=255)                                                           ccTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACg                                                                              >  6:29462/1‑149 (MQ=255)                                                            cTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGt                                                                             >  2:221413/1‑149 (MQ=255)                                                               ttGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGAc                                                                          >  2:112726/1‑149 (MQ=255)                                                                  gCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCGTGGGCGT‑TCGACGTGACCgg                                                                       >  4:125109/1‑149 (MQ=255)                                                                  gCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGa                                                                           <  5:309526/145‑1 (MQ=255)                                                                  gCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGa                                                                           >  6:309526/1‑145 (MQ=255)                                                                  gCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCgg                                                                       >  2:280081/1‑149 (MQ=255)                                                                  gCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCgg                                                                       <  7:63388/149‑1 (MQ=255)                                                                   cccTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCgg                                                                       >  4:310951/1‑148 (MQ=255)                                                                    ccTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCggg                                                                      >  5:331299/1‑148 (MQ=255)                                                                    ccTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCggg                                                                      >  3:122411/1‑148 (MQ=255)                                                                     cTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGaa                                                                    <  6:156814/149‑1 (MQ=255)                                                                          ggTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATg                                                                <  5:18688/148‑1 (MQ=255)                                                                           gTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGAt                                                              <  3:310951/149‑1 (MQ=255)                                                                              aGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATccc                                                           >  1:35371/1‑149 (MQ=255)                                                                              aGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATcc                                                            <  8:179294/148‑1 (MQ=255)                                                                                 gTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGATCATGTTCCCGGt                                                        >  6:95409/1‑149 (MQ=255)                                                                                 gTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGt                                                        >  2:65705/1‑149 (MQ=255)                                                                                  tCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGATCATGTTCCCGGTc                                                       <  5:95409/149‑1 (MQ=255)                                                                                   cAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCa                                                      >  3:76106/1‑149 (MQ=255)                                                                                    aaCGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGATCATGTTCCCGGTCAc                                                     <  4:243625/149‑1 (MQ=255)                                                                                     aCGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACg                                                    <  1:36489/149‑1 (MQ=255)                                                                                      cGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACgg                                                   <  8:49638/149‑1 (MQ=255)                                                                                          ggCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCGTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGa                                               <  3:125109/149‑1 (MQ=255)                                                                                           gcgcCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTc                                                 >  5:348231/1‑146 (MQ=255)                                                                                           gcgcCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTc                                                 <  6:348231/146‑1 (MQ=255)                                                                                               ccACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTGACGACGTTACCGGGAACAAGATCCCGGTCAGGGTGAAgcgc                                           >  2:355982/1‑149 (MQ=255)                                                                                                cACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCt                                         >  5:301191/1‑149 (MQ=255)                                                                                                 aCGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTg                                        >  4:355429/1‑149 (MQ=255)                                                                                                 aCGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTg                                        >  5:7687/1‑149 (MQ=255)                                                                                                     ccTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCgg                                    <  4:122411/149‑1 (MQ=255)                                                                                                        gtgGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGcc                                          <  8:343141/140‑1 (MQ=255)                                                                                                        gtgGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGcc                                          >  7:343141/1‑140 (MQ=255)                                                                                                         tgGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATc                                 <  1:65705/148‑1 (MQ=255)                                                                                                         tgGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCa                                >  5:17673/1‑149 (MQ=255)                                                                                                         tgGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCa                                >  7:227152/1‑149 (MQ=255)                                                                                                           gA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCGTGGGGGT‑TCGTCGTGACCGGGAACATGATCCCGTTCACGGTCGAGCGCCTGGCGGATCAt                               <  6:138655/148‑1 (MQ=255)                                                                                                           gA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATg                              >  5:196562/1‑149 (MQ=255)                                                                                                           gA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATg                              <  5:123664/149‑1 (MQ=255)                                                                                                               cGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCg                           >  8:106945/1‑149 (MQ=255)                                                                                                               cGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCg                           >  4:122776/1‑149 (MQ=255)                                                                                                                   gagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgct                       >  3:71994/1‑149 (MQ=255)                                                                                                                     gCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgctgc                     <  7:242453/149‑1 (MQ=255)                                                                                                                       gACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCaa                   <  1:221413/149‑1 (MQ=255)                                                                                                                       gACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCaa                   <  7:106945/149‑1 (MQ=255)                                                                                                                           tGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCAc               <  1:112726/149‑1 (MQ=255)                                                                                                                              cAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTa            >  1:97706/1‑149 (MQ=255)                                                                                                                               aaTATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCCCttgc           >  4:282711/1‑147 (MQ=255)                                                                                                                                  atCGAGGCCGTGTACAGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTAcccc        >  4:321517/1‑149 (MQ=255)                                                                                                                                   ccgtggCCGTACACGGCCACGGCGCCGAGGATAACGCCTACAAGGGGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACGTAcc          >  7:300699/5‑146 (MQ=255)                                                                                                                                   ccgtggCCGTACACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCg       >  1:30234/5‑149 (MQ=255)                                                                                                                                    cGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgctgc                     <  1:147110/134‑1 (MQ=255)                                                                                                                                     gtggCCGTACACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgc                        <  1:291995/128‑1 (MQ=255)                                                                                                                                     gtggCCGTACACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgc                        >  2:291995/3‑130 (MQ=255)                                                                                                                                     gAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGcc     >  7:257664/1‑149 (MQ=255)                                                                                                                                        gCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGc  <  6:7687/149‑1 (MQ=255)                                                                                                                                        gCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGc  <  6:196562/149‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                 CTGACCCTGAGCCTTTGAACCGAGGAACCATCATGCATCCATCGCTGAAAGTCGCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGA‑CCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGT‑TCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGC  >  NC_002947/3762726‑3763008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑