Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,770,822	A→C	6.1%	T79P (ACC→CCC)	PP_3331 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,770,822	0	A	C	6.1%	232.0 / 2.6	75	T79P (ACC→CCC)	PP_3331	membrane protein
Reads supporting (aligned to +/- strand):  ref base A (36/34);  new base C (3/2);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTGGCCAATGCCCGGCAGTATGCCGGTGAGG  >  NC_002947/3770678‑3770963                                                                                                                                                 |                                                                                                                                                  gTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAg                                                                                                                                               >  4:86239/1‑149 (MQ=255) gTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCCGCCCAg                                                                                                                                               >  3:110843/1‑149 (MQ=255)  tctcCGGGGTTGTCATGCTGTACCTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGc                                                                                                                                              <  7:123885/149‑1 (MQ=255)    tcCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCt                                                                                                                                            >  7:190033/1‑149 (MQ=255)          gTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTgggcgg                                                                                                                                      <  7:209539/149‑1 (MQ=255)             gTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCCGCCCAGGCTggtcaggc                                                                                                                                    <  4:110843/148‑1 (MQ=255)              tCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTgggcgg                                                                                                                                      <  8:348046/145‑1 (MQ=255)              tCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTgggcgg                                                                                                                                      >  7:348046/1‑145 (MQ=255)              tCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGgcgc                                                                                                                                  >  3:154467/1‑149 (MQ=255)                aTGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGCCCAGCCTGGGCGGgcgcgc                                                                                                                                >  2:46749/1‑149 (MQ=255)                 tGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGgcgcgcg                                                                                                                               >  3:209011/1‑149 (MQ=255)                    actACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑cac                                                                                                                        <  2:328728/147‑1 (MQ=255)                           cGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACctgc                                                                                                                 <  8:11607/149‑1 (MQ=255)                            ggCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACctgct                                                                                                                <  6:192623/149‑1 (MQ=255)                              cTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTgg                                                                                                              <  8:190033/149‑1 (MQ=255)                                 ccccAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAAc                                                                                                           <  4:209011/149‑1 (MQ=255)                                  cccAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACt                                                                                                          >  3:152039/1‑149 (MQ=255)                                     aaGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGc                                                                                                       <  3:86239/149‑1 (MQ=255)                                      aGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTgggcgg                                                                                                                                      >  7:43578/1‑121 (MQ=255)                                      aGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTgggcgg                                                                                                                                      <  8:43578/121‑1 (MQ=255)                                        cTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGa                                                                                                    <  6:126858/149‑1 (MQ=255)                                          gACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCGGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACg                                                                                                  >  7:195451/1‑149 (MQ=255)                                             acCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTc                                                                                               >  1:185009/1‑149 (MQ=255)                                               cGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTcgc                                                                                             >  5:128595/1‑149 (MQ=255)                                                   cGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGTGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCcgcg                                                                                         >  1:69309/1‑149 (MQ=255)                                                   cGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCcgcg                                                                                         >  6:380096/1‑149 (MQ=255)                                                    gAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGt                                                                                        >  3:355390/1‑149 (MQ=255)                                                         ccTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGGGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATcc                                                                                   >  1:117798/1‑149 (MQ=255)                                                         ccTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATc                                                                                    >  2:141004/1‑148 (MQ=255)                                                                cACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATg                                                                             >  8:291448/1‑148 (MQ=255)                                                                cACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATg                                                                             <  7:291448/148‑1 (MQ=255)                                                                 aCCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATgcc                                                                           >  1:329982/1‑149 (MQ=255)                                                                   cTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATgccgc                                                                         >  2:304006/1‑149 (MQ=255)                                                                       cGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAgc                                                                     >  2:165044/1‑149 (MQ=255)                                                                        ggCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGa                                                                                                                                                   >  5:238346/1‑74 (MQ=255)                                                                               ccGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGc                                                              <  1:165044/148‑1 (MQ=255)                                                                                cggcggGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCct                                                            <  7:259953/149‑1 (MQ=255)                                                                                   cggGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGGGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTg                                                         <  2:117798/149‑1 (MQ=255)                                                                                    ggggTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTgg                                                        >  6:379790/1‑149 (MQ=255)                                                                                       gggcggcgcCGAGTTGCCCGAGCATTGGGCGCAACCGCCGCTGACGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGCAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTc                                                     <  4:355390/144‑1 (MQ=255)                                                                                        tgctgcGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATg                                                                             >  6:292865/1‑124 (MQ=255)                                                                                        tgctgcGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATg                                                                             <  5:292865/124‑1 (MQ=255)                                                                                          ctgcGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCgg                                                   <  2:69309/148‑1 (MQ=255)                                                                                                cGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTTGAACTGGCCTACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACg                                            <  6:351124/149‑1 (MQ=255)                                                                                                  aGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCTACGCa                                          <  1:152867/149‑1 (MQ=255)                                                                                                  aGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCa                                          <  6:128595/149‑1 (MQ=255)                                                                                                   gTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGCCGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAg                                         >  8:102225/1‑149 (MQ=255)                                                                                                    ttGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGc                                        <  7:41623/149‑1 (MQ=255)                                                                                                        ccGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTgg                                    >  1:292432/1‑149 (MQ=255)                                                                                                        ccGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTgg                                    >  7:222211/1‑149 (MQ=255)                                                                                                         cGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGc                                                                                                       >  6:23135/1‑81 (MQ=255)                                                                                                         cGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGc                                                                                                       <  5:23135/81‑1 (MQ=255)                                                                                                         cGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTggg                                   >  2:254136/1‑149 (MQ=255)                                                                                                          gAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGc                                  >  7:154286/1‑149 (MQ=255)                                                                                                            gCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGccc                                >  6:248869/1‑149 (MQ=255)                                                                                                              aTTGGGCGCAACAGCCGCGGAAGGCGCTGCGCCTGCCCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTg                              <  1:46749/149‑1 (MQ=255)                                                                                                               ttGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTgg                             >  5:277915/1‑149 (MQ=255)                                                                                                                 gggCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTGGc                            <  7:102225/148‑1 (MQ=255)                                                                                                                  ggCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTGGcc                           >  6:187140/1‑148 (MQ=255)                                                                                                                   gcgcAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCCCTCCCCACCCCCCCCCCCTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTgg                             <  1:304006/149‑1 (MQ=255)                                                                                                                   gcgcAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTGGCCaa                         >  6:362756/1‑149 (MQ=255)                                                                                                                     gcAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTg                              <  2:269083/142‑1 (MQ=255)                                                                                                                     gcAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTg                              >  1:269083/1‑142 (MQ=255)                                                                                                                        aCAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTGGCCAATGccc                    <  2:329982/149‑1 (MQ=255)                                                                                                                        aCAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCCCTGGCTCGGGCCGACGCAGCCTGGGCCCTGGCCAATGccc                    >  6:282530/1‑149 (MQ=255)                                                                                                                          aGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTGGCCAATGCCCgg                  >  6:72826/1‑149 (MQ=255)                                                                                                                           gCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGcc                                                <  7:310357/118‑1 (MQ=255)                                                                                                                           gCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGcc                                                >  8:310357/1‑118 (MQ=255)                                                                                                                                    ggcgcggcgcCGGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTGGCCAATGCCCGGCAGTATGCCg        <  8:252544/149‑1 (MQ=255)                                                                                                                                     gcgcTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCGGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTGGCCAATGCCCGGCAGTATGCCgg       <  7:342606/149‑1 (MQ=255)                                                                                                                                        cTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTGGCCAATGCCCGGCAGTATGCCGGTGa    <  5:248869/149‑1 (MQ=255)                                                                                                                                        cTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTGGCCAATGCCCGGCAGTATGCCGGTGa    <  4:120899/149‑1 (MQ=255)                                                                                                                                        cTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTGGCCAATGCCCGGCAGTATGCCGGTGa    <  8:195451/149‑1 (MQ=255)                                                                                                                                          gcgcCCGCCCAGGCTGGTCAGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATGCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTGGCCAATGCCCGGCAGTATGCCGGTGAgg  >  1:354871/1‑149 (MQ=255)                                                                                                                                               tGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTGGCCAATGCCCGGCAg               <  8:178096/131‑1 (MQ=255)                                                                                                                                               tGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTGGCCAATGCCCGGCAg               >  7:178096/1‑131 (MQ=255)                                                                                                                                                 |                                                                                                                                                  GTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCG‑‑‑‑CACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTGGCCAATGCCCGGCAGTATGCCGGTGAGG  >  NC_002947/3770678‑3770963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑