Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,872,030 G→T 5.6% F117L (TTC→TTA) ‡ PP_3420 ← sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,872,0300GT 5.6% 224.2 / 3.1 71F117L (TTC→TTA) ‡PP_3420sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (30/37);  new base T (2/2);  total (32/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

GCGCGAGCGGAAGGCGCGCTCAAGATCGCCATCAGCCTCTATCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGAATGCCGCACTTCGGCCTGGGCCATTGCCTGAATGAAC  >  NC_002947/3871886‑3872174
                                                                                                                                                |                                                                                                                                                
gcgcGAGCGGAAGGCGCGCTCAAGATCGCCATCAGCCTCTATCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGaa                                                                                                                                                <  2:356762/147‑1 (MQ=255)
     aGCGGAAGGCGCGCTCAAGATCGCCATCAGCCTCTATCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACAGGATCGAGTTCCTCGATc                                                                                                                                         <  5:240888/149‑1 (MQ=255)
         gAAGGCGCGCTCAAGATCGCCATCAGCCTCTATCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCggg                                                                                                                                     >  3:112543/1‑149 (MQ=255)
         gAAGGCGCGCTCAAGATCGCCATCAGCCTCTATCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCggg                                                                                                                                     <  2:29505/149‑1 (MQ=255)
              cgcgcTCAAGATCGCCATCAGCCTCTATCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGAt                                                                                                                                <  5:269425/149‑1 (MQ=255)
                  cTCAAGATCGCCATCAGCCTCTATCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAAc                                                                                                                                               >  3:84864/1‑130 (MQ=255)
                  cTCAAGATCGCCATCAGCCTCTATCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAAc                                                                                                                                               <  4:84864/130‑1 (MQ=255)
                     aaGATCGCCATCAGCCTCTATCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGc                                                                                                                           >  7:105938/1‑147 (MQ=255)
                     aaGATCGCCATCAGCCTCTATCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGc                                                                                                                           >  1:367434/1‑147 (MQ=255)
                       gATCGCCATCAGCCTCTATCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAgg                                                                                                                       <  1:170718/149‑1 (MQ=255)
                       gATCGCCATCAGCCTCTATCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAgg                                                                                                                       <  6:168330/149‑1 (MQ=255)
                           gCCATCAGCCTCTATCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCg                                                                                                                   <  3:149292/149‑1 (MQ=255)
                            ccATCAGCCTCTATCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCgg                                                                                                                  <  3:337515/149‑1 (MQ=255)
                             cATCAGCCTCTATCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGGCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGGGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCggg                                                                                                                 <  5:185027/149‑1 (MQ=255)
                               tCAGCCTCTATCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGt                                                                                                               >  5:294101/1‑149 (MQ=255)
                                      ctATCCGCTGGCCAATCAGCTCCACGTGGGTGGACCAGACAATGGTGCGGTCTCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGc                                                                                                        <  5:217089/149‑1 (MQ=255)
                                         tCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGc                                                                                                        <  8:130577/146‑1 (MQ=255)
                                         tCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAg                                                                                                     >  4:14490/1‑149 (MQ=255)
                                                gCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGtgctg                                                                                                >  1:179422/1‑147 (MQ=255)
                                                 ccAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAg                                                                                             <  5:96458/149‑1 (MQ=255)
                                                  cAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGt                                                                                                                                    >  5:24013/1‑109 (MQ=255)
                                                  cAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGt                                                                                                                                    <  6:24013/109‑1 (MQ=255)
                                                   aaTCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGt                                                                                           >  7:75637/1‑149 (MQ=255)
                                                     tCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTc                                                                                          >  1:113208/1‑148 (MQ=255)
                                                        gCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGc                                                                                      >  1:167579/1‑149 (MQ=255)
                                                          tCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCcg                                                                                    >  2:281442/1‑149 (MQ=255)
                                                             aCGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCgg                                                                                 <  8:295368/149‑1 (MQ=255)
                                                                ttGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTc                                                                              <  8:105938/149‑1 (MQ=255)
                                                                ttGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTc                                                                              <  6:294101/149‑1 (MQ=255)
                                                                   gtggACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAg                                                                           <  2:367434/149‑1 (MQ=255)
                                                                   gtggACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATCCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAg                                                                           <  1:281442/149‑1 (MQ=255)
                                                                      gACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGtgct                                                                                                 >  2:53608/1‑124 (MQ=255)
                                                                      gACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCaa                                                                        <  7:346094/149‑1 (MQ=255)
                                                                      gACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCaa                                                                        >  2:185006/1‑149 (MQ=255)
                                                                      gACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGGCGATTCGGCCAGGGCCGGGGTGACCTGCAAGtgct                                                                                                 <  1:53608/124‑1 (MQ=255)
                                                                         cAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTc                                                                     >  4:131720/1‑149 (MQ=255)
                                                                         cAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTc                                                                     >  1:36888/1‑149 (MQ=255)
                                                                         cAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTc                                                                     >  4:286614/1‑149 (MQ=255)
                                                                             cAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGccc                                                                 <  7:91635/149‑1 (MQ=255)
                                                                              aaTGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCa                                                                >  2:282437/1‑149 (MQ=255)
                                                                              aaTGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCa                                                                >  7:261391/1‑149 (MQ=255)
                                                                                tGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCAtg                                                              >  3:174832/1‑149 (MQ=255)
                                                                                 ggTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCAtgg                                                             >  1:323539/1‑149 (MQ=255)
                                                                                  gTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCAtggt                                                            <  5:289470/149‑1 (MQ=255)
                                                                                      ggTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGgtggtg                                                        <  8:75637/149‑1 (MQ=255)
                                                                                           cgGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAgg                                                   <  1:185006/149‑1 (MQ=255)
                                                                                            gggCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAggc                                                  <  1:39993/149‑1 (MQ=255)
                                                                                                aTAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAggcggc                                               >  3:38422/1‑148 (MQ=255)
                                                                                                  aGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAAt                                            >  2:64094/1‑149 (MQ=255)
                                                                                                       ttGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGa                                       <  3:14490/149‑1 (MQ=255)
                                                                                                            cAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGAATGcc                                  <  1:282437/149‑1 (MQ=255)
                                                                                                              gCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGAATGCCg                                 >  1:42295/1‑148 (MQ=255)
                                                                                                               cAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGAATGCCGCa                               <  3:286614/149‑1 (MQ=255)
                                                                                                               cAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGAATGCCGCa                               <  4:112543/149‑1 (MQ=255)
                                                                                                                 aGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGAATGCCGCACt                             >  3:269088/1‑149 (MQ=255)
                                                                                                                 aGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGAATGCCGCACt                             >  4:152887/1‑149 (MQ=255)
                                                                                                                   gtgtCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGAATGCCGCACTTc                           >  7:54003/1‑149 (MQ=255)
                                                                                                                      tCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGAATGCCGCACTTCGGc                        <  4:174832/149‑1 (MQ=255)
                                                                                                                        cGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGc                                         <  8:47071/130‑1 (MQ=255)
                                                                                                                        cGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGc                                         >  7:47071/1‑130 (MQ=255)
                                                                                                                              gTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAg                                                                                       <  6:113701/78‑1 (MQ=255)
                                                                                                                              gTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAg                                                                                       >  5:113701/1‑78 (MQ=255)
                                                                                                                                   cGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGAATGCCGCACTTCGGCCTGGGCCATTGcc           >  5:13069/1‑149 (MQ=255)
                                                                                                                                    gACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGAATGCCGCACTTCGGCCTGGGCCATTGCCt          <  4:269088/149‑1 (MQ=255)
                                                                                                                                      ccggATCGAGTTCCTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTc                                                                     <  2:25279/85‑1 (MQ=255)
                                                                                                                                      ccggATCGAGTTCCTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTc                                                                     >  1:25279/4‑88 (MQ=255)
                                                                                                                                       cggATCGAGTTCCTCGATCCCGGTCGATTCGGCCAGCGCCGGGGTGCCCTGCAAGTGCTGCAGGTCCACCCGCCGCTCCAGCAACTCGCCCATGGTCGTGCCAGGCGGCAAATGCGAAAGCCGCACTACGGCCTGGGCCAATGCCtgaa       >  2:323214/3‑149 (MQ=255)
                                                                                                                                       aTGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGAATGCCGCACTTCGGCCTGGGCCATTGCCtgaa       <  2:42295/149‑1 (MQ=255)
                                                                                                                                       aTGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGAATGCCGCACTTCGGCCTGGGCCATTGCCtgaa       <  6:13069/149‑1 (MQ=255)
                                                                                                                                          aTCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGAATGCCGCACTTCGGCCTGGGCCATTGCCtgaatg     <  8:261391/148‑1 (MQ=255)
                                                                                                                                             gAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGAATGCCGCACTTCGGCCTGGGCCATTGCCTGAATGAAc  <  2:167579/148‑1 (MQ=255)
                                                                                                                                               ggAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGAATGCCGCACTTCg                          <  1:252355/122‑1 (MQ=255)
                                                                                                                                               ggAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGAATGCCGCACTTCg                          >  2:252355/1‑122 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GCGCGAGCGGAAGGCGCGCTCAAGATCGCCATCAGCCTCTATCCGCTTGCCAATCAGCTCCACGTTGGTGGACCAGACAATGGTGCGGTCGCGGGCATAGACATTGGCCAGCAAGGTGTCCGGCAGGTGCTCGACATGATCGAGGAACTCGATCCGGGTCGATTCGGCCAGGGCCGGGGTGACCTGCAAGTGCTGCAGGTCCAGCCGCGGGTCCAGCAACTCGCCCATGGTGGTGCCAGGCGGCAATTGCGAATGCCGCACTTCGGCCTGGGCCATTGCCTGAATGAAC  >  NC_002947/3871886‑3872174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑