Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,911,732 A→T 6.7% E26D (GAA→GAT)  PP_3452 → GGDEF domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,911,7320AT 6.7% 165.6 / 2.9 60E26D (GAA→GAT) PP_3452GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (25/31);  new base T (2/2);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.69e-01

TCCCTGTAGGAGCGGGTTTACCCGCGAAAGGTCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCG  >  NC_002947/3911586‑3911880
                                                                                                                                                  |                                                                                                                                                    
tCCCTGTAGGAGCGGTTTTACCCGCGAAAGGTCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCGGACCGATGTGCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAAtt                                                                                                                                                    <  7:194688/149‑1 (MQ=255)
  ccTGTAGGAGCGGGTTTACCCGCGAAAGGTCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTc                                                                                                                                                   >  1:12187/1‑148 (MQ=255)
  ccTGTAGGAGCGGGTTTACCCGCGAAAGGTCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTc                                                                                                                                                   >  7:48644/1‑148 (MQ=255)
  ccTGTAGGAGCGGGTTTACCCGCGAAAGGTCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTc                                                                                                                                                   <  8:196583/148‑1 (MQ=255)
  ccTGTAGGAGCGGGTTTACCCGCGAAAGGTCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCg                                                                                                                                                  <  8:221832/149‑1 (MQ=255)
  ccTGTAGGAGCGGGTTTACCCGCGAAAGGTCCAAGCCTGAAAAAGTAGTACCCTCAGGAGCCCACCGATGATCAAGACCATCGAAGCCCACATACGCCAGAAGGTCGCCCCCGCCGCCAAGCGCGCCGACTTCCCCCAGCACCAATTc                                                                                                                                                   >  7:130092/1‑148 (MQ=255)
   cagTAGGAGGGTGTTTACCCGCGAAAGGTCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGa                                                                                                                                                 <  5:324957/147‑1 (MQ=255)
    tGTAGGAGCGGGTTTACCCGCGAAAGGTCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAg                                                                                                                                                >  6:92947/1‑149 (MQ=255)
      tAGGAGCGGGTTTACCCGCGAAAGGTCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGt                                                                                                                                               <  1:232141/148‑1 (MQ=255)
       tttcgCGGGTAAACCCGCGAAAGGTCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTc                                                                                                                                              >  3:96468/5‑148 (MQ=255)
           gCGGGTTTACCCGCGAAAGGTCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATgc                                                                                                                                         <  2:162152/149‑1 (MQ=255)
              ggTTTACCCGCGAAAGGTCTAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCt                                                                                                                                      >  7:266527/1‑149 (MQ=255)
              ggTTTACCCGCGAAAGGTCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCCCCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCt                                                                                                                                      >  6:214381/1‑149 (MQ=255)
               gTTTACCCGCGAAAGGTCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTc                                                                                                                                     >  2:162893/1‑149 (MQ=255)
                 ttACCCGCGAAAGGTCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGGCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCGCGt                                                                                                                                   <  8:209924/149‑1 (MQ=255)
                     ccGCGAAAGGTCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTctg                                                                                                                               >  8:205466/1‑149 (MQ=255)
                         gAAAGGTCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTctgcctg                                                                                                                           <  6:341542/149‑1 (MQ=255)
                            aGGTCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCctgctg                                                                                                                        <  5:92947/149‑1 (MQ=255)
                               tCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCctgctg                                                                                                                        <  2:169447/146‑1 (MQ=255)
                               tCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCctgctg                                                                                                                        >  1:169447/1‑146 (MQ=255)
                                   aGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATtct                                                                                                                 <  7:205466/149‑1 (MQ=255)
                                    gCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATtctc                                                                                                                <  2:41470/149‑1 (MQ=255)
                                      cTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGa                                                                                                              <  8:20105/149‑1 (MQ=255)
                                         aaaaaGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGAt                                                                                                             <  8:48644/147‑1 (MQ=255)
                                         aaaaaGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCa                                                                                                           <  7:226594/149‑1 (MQ=255)
                                                    gCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGTTATTCTCGATCAGCGTCCTGAt                                                                                                 <  4:160400/148‑1 (MQ=255)
                                                    gCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATc                                                                                                >  7:261132/1‑149 (MQ=255)
                                                    gCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATc                                                                                                <  2:12187/149‑1 (MQ=255)
                                                         aGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATctggct                                                                                           <  1:162893/149‑1 (MQ=255)
                                                              cccACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTc                                                                                                                                     <  5:53327/102‑1 (MQ=255)
                                                              cccACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTc                                                                                                                                     >  6:53327/1‑102 (MQ=255)
                                                                cACCGATGTTCAAGACCATCTAAGCCCACATAAGACATCAGGTCGCGCCCTCCGCAATGAGCGCCGAGTTCAGCCAGCACGATTTCTAGTCCATGAGCTCGTTctgc                                                                                                                              >  4:281039/1‑107 (MQ=255)
                                                                cACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGATTTCGAGTCCATGCGCTCGTTctgc                                                                                                                              <  3:281039/107‑1 (MQ=255)
                                                                cACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGATTTCGAGTCCATGCGCTCGTTctgc                                                                                                                              <  3:286033/107‑1 (MQ=255)
                                                                cACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGATTTCGAGTCCATGCGCTCGTTctgc                                                                                                                              >  4:286033/1‑107 (MQ=255)
                                                                cACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCtag                                                                                    >  3:291210/1‑147 (MQ=255)
                                                                 aCCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTc                                                                                    >  1:1822/1‑148 (MQ=255)
                                                                   cGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGAc                                                                                 >  4:301481/1‑149 (MQ=255)
                                                                           aaGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGt                                                                         <  1:169803/149‑1 (MQ=255)
                                                                             gACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGt                                                                         >  6:327257/1‑147 (MQ=255)
                                                                             gACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCa                                                                       <  8:266527/149‑1 (MQ=255)
                                                                                     aaGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTc                                                               <  4:96468/149‑1 (MQ=255)
                                                                                                cGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAgg                                                    >  1:27477/1‑149 (MQ=255)
                                                                                                   cagcagGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCtt                                                 >  5:252571/1‑149 (MQ=255)
                                                                                                   cagcagGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCtt                                                 >  3:77761/1‑149 (MQ=255)
                                                                                                    agcagGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCtt                                                 >  6:310843/1‑148 (MQ=255)
                                                                                                                 cgccgcCCTGCGCGCCAAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCTTCACCTGGCTGtcct                                   >  3:144805/1‑149 (MQ=255)
                                                                                                                   ccgccCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCTTCACCTGGCTGtccttc                                 <  2:287423/149‑1 (MQ=255)
                                                                                                                   ccgccCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCTTCACCTGGCTGtccttc                                 <  3:301481/149‑1 (MQ=255)
                                                                                                                     gccCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCTTCACCTGGCTGtccttcct                               <  5:327257/149‑1 (MQ=255)
                                                                                                                       ccTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCTTCACCTGGCAGTCCTTCCTGt                             >  7:330709/1‑149 (MQ=255)
                                                                                                                       ccTGCGCGCCGAGTTCCGCCAGCACGAAATCCACGCCATGCGCTCGTTCTGCCTTGTGGTATTCTCGATCAGCGTCCTGATCTTGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGt                             <  8:261132/149‑1 (MQ=255)
                                                                                                                           cgcgcCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCa                          <  5:310843/148‑1 (MQ=255)
                                                                                                                           cgcgcCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCAt                         <  6:252571/149‑1 (MQ=255)
                                                                                                                           cgcgcCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCAt                         <  4:77761/149‑1 (MQ=255)
                                                                                                                                   gTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGc                 <  5:214381/149‑1 (MQ=255)
                                                                                                                                      ccGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATc                        >  8:157797/1‑139 (MQ=255)
                                                                                                                                      ccGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATc                        <  7:157797/139‑1 (MQ=255)
                                                                                                                                             cACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCt       >  6:74331/1‑149 (MQ=255)
                                                                                                                                                gAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGAt    >  3:206723/1‑149 (MQ=255)
                                                                                                                                                  aTTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCg  <  4:206723/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TCCCTGTAGGAGCGGGTTTACCCGCGAAAGGTCCAAGCCTGAAAAAGTAGTAGCCTCAGGAGCCCACCGATGTTCAAGACCATCGAAGCCCACATACGCCAGCAGGTCGCGCCCGCCGCCCTGCGCGCCGAGTTCCGCCAGCACGAATTCGAGTCCATGCGCTCGTTCTGCCTGCTGGTATTCTCGATCAGCGTCCTGATCTGGCTGGTCTTCGACCTGATCGTCAGCTTCCTCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCG  >  NC_002947/3911586‑3911880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑