Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,005,171 G→A 9.7% G290G (GGC→GGT)  PP_3534 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,005,1710GA 9.7% 109.0 / 2.7 42G290G (GGC→GGT) PP_3534LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (19/18);  new base A (2/2);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGAGGCCGCGCTGGCAAAAGGCGTATCCGACGGGTTGCCTGATATTGAATCGGGTTGGTCGAAAGTGTGTGTGTGGGCTTTGGGTCATGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGCCCGGCCCCCAACACCCTGTGCTCGCCGGTGGCCGCCCTG  >  NC_002947/4005045‑4005318
                                                                                                                              |                                                                                                                                                   
ccGAGGCCGCGCTGGCAAAAGGCGTATCCGACGGGTTGCCTGATATTGATTCGGGTTGGTCGATAGTGTGTGTGTGGGCTTTGGGTCTTGTACAAGCGCATCGACGACAGATCGTCAGCTCCCCCCACCCGCCAGCAACTGCGtcttca                                                                                                                               >  4:281881/1‑149 (MQ=255)
ccGAGGCCGCGCTGGCAAAAGGCGTATCCGACGGGTTGCCTGATATTGAATCGGGTTGGTCGAAAGTGTGTGTGTGGGCTTTGGGTCATGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCACCCGCCAGCACCTGCGtcatca                                                                                                                               >  4:281878/1‑149 (MQ=255)
 cGAGGCCGCGCTGGCAAAAGGCGTATCCGACGGGTTGCCTGATATTGAATCGGGTTGGTCGAAAGTGTGTGTGTGGGCTTTGGGTCATGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCAc                                                                                                                              >  5:142202/1‑149 (MQ=255)
  gAGGCCGCGCTGGCAAAAGGCGTATCCGACGGGTTGCCTGATATTGAATCGGGTTGGTCGAAAGTGTGTGTGTGGGCTTTGGGTCATGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCAcc                                                                                                                             >  7:78664/1‑149 (MQ=255)
              gCAAAAGGCGTATCCGACGGGTTGCCTGATATTGAATCGGGTTGGTCGAAAGTGTGTGTGTGGGCTTTGGGTCATGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAgcgc                                                                                                                  <  4:311594/148‑1 (MQ=255)
                 aaaGGCGTATCCGACGGGTTGCCTGATATTGAATCGGGTTGGTCGAAAGTGTGTGTGTGGGCTTTGGGTCATGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTg                                                                                                              <  3:208805/149‑1 (MQ=255)
                          tCCGACGGGTTGCCTGATATTGAATCGGGTTGGTCGAAAGTGTGTGTGTGGGCTTTGGGTCATGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGtc                                                                                                                                   <  3:215214/119‑1 (MQ=255)
                          tCCGACGGGTTGCCTGATATTGAATCGGGTTGGTCGAAAGTGTGTGTGTGGGCTTTGGGTCATGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGtc                                                                                                                                   >  4:215214/1‑119 (MQ=255)
                           ccGACGGGTTGCCTGATATTGAATCGGGTTGGTCGAAAGTGTGTGTGTGGGCTTTGGGTCATGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACAt                                                                                                    >  8:187943/1‑149 (MQ=255)
                           ccGACGGGTTGCCTGATATTGAATCGGGTTGGTCGAAAGTGTGTGTGTGGGATTTGGGTCATGTACAAGCGCATCGACAACAGATCGTCAGATCCCACCGCCCGCCAGCAACTCCGTCATCACCTCAGCCAGCGCCTTGACCATcaca                                                                                                     >  4:259370/1‑148 (MQ=255)
                             gACGGGTTGCCTGATATTGAATCGGGTTGGTCGAAAGTGTGTGTGTTGGGTTTGGGTGATGTACAAGCGCATCGACCACAGATCGTCAGATCGCACCGCCCGGCAGGATCTGCGTCATCTGCTca                                                                                                                          <  2:177975/125‑1 (MQ=255)
                                        tGATATTGAATCGGGTTGGTCGAAAGTGTGTGTGTGGGCTTTGGGTCATGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCg                                                                                       >  4:149783/1‑149 (MQ=255)
                                          atatTGAATCGGGTTGGTCGAAAGTGTGTGTGTGGGCTTTGGGTCATGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGt                                                                                     <  8:78664/149‑1 (MQ=255)
                                          atatTGAATCGGGTTGGTCGAAAGTGTGTGTGTGGGCTTTGGGTCATGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGt                                                                                     <  3:149783/149‑1 (MQ=255)
                                             ttGAATCGGGTTGGTCGAAAGTGTGTGTGTGGGCTTTGGGTCATGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCa                                                                                  >  4:134856/1‑149 (MQ=255)
                                             ttGAATCGGGTTGGTCGAAAGTGTGTGTGTGGGCTTTGGGTCATGTACAAGCGCATCGACCAAAGATCGTCAGATACCACCGCCCGACAGCAACTGCGTCATCCACTCAGCCAGCGCCTTGACCATCACATCCGCCGTAGGACGGTgaa                                                                                  >  4:278774/1‑147 (MQ=255)
                                                         tgTCGAAAGTGTGTGTGTGGGCTTTGGGTCATGTACAAGCGCATCGACCACAGATCGTCAGATCCCAGCGCCCGCCTGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCAGGATGACGAtc                                                                       <  7:344768/147‑1 (MQ=255)
                                                               aaGTGTGTGTGTGGGCTTTGGGTCATGTACAATCGCATCGACCACAGATCGTCAGATCCCACCCCACGCCAGCAACTGCGTAATCACATCAGCCAGCGCCTTGAACATAACATACGCCGTAGGCCGTTGCAAGATGACGATCAAATAGc                                                                >  5:211578/1‑149 (MQ=255)
                                                                     gtgtgtgGGCTTTGGGTCATGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGa                                                          >  6:137456/1‑149 (MQ=255)
                                                                          tgGGCTTTGGGTCATGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTcc                                                     >  6:358327/1‑149 (MQ=255)
                                                                               tttGGGTCATGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAg                                                >  2:150878/1‑149 (MQ=255)
                                                                                       aTGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGcc                                        <  3:134856/149‑1 (MQ=255)
                                                                                       aTGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGc                                         <  1:150878/148‑1 (MQ=255)
                                                                                       aTGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGc                                         <  6:142202/148‑1 (MQ=255)
                                                                                          tACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGCCCgg                                     >  3:196564/1‑149 (MQ=255)
                                                                                          tACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGCCCgg                                     >  5:245449/1‑149 (MQ=255)
                                                                                          tACAAGCGCATCGACCACAGATCGTCAGATCCCACCACCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGCCCgg                                     <  3:281881/149‑1 (MQ=255)
                                                                                           aCAAGCGCATCGACCACAGATCGTCAGATACCACCACCCGCCATCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCAAAGCTGTCGACCTCCGGCAGCCCTTGCCCgg                                     <  3:281878/148‑1 (MQ=255)
                                                                                            cAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGCCCGGcc                                   <  4:196564/149‑1 (MQ=255)
                                                                                             aaGCGGATCGACCAGAGATAGTGAGATCCCACCGGCCGCCAGCAAGTGCGTCATGACCTCAGCCAGCGCGTTGACCATCACATGGGCCTTTTGCCGGTGCACGATGACGATGTCATAGCTGTCGACCTCCGGCTGCCCTTGCGCGGacc                                  <  6:211578/149‑1 (MQ=255)
                                                                                             aaGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATa                                                                  <  3:209262/117‑1 (MQ=255)
                                                                                             aaGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATa                                                                  >  4:209262/1‑117 (MQ=255)
                                                                                                   aTCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGCCCGGCCCCCAaca                            >  5:191472/1‑149 (MQ=255)
                                                                                                         cacaGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGc                                         >  4:95678/1‑130 (MQ=255)
                                                                                                         cacaGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGc                                         <  3:95678/130‑1 (MQ=255)
                                                                                                            aGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGCCCGGCCCCCAACACCCTGTGCt                   >  3:38024/1‑149 (MQ=255)
                                                                                                               tCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGCCCGGCCCCCAACACCCTGTGCTCGc                <  7:187943/149‑1 (MQ=255)
                                                                                                               tCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGCCCGGCCCCCAACACCCTGTGCTCGc                <  3:259370/149‑1 (MQ=255)
                                                                                                                    aGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGCCCGGCCCCCAacac                           <  3:326502/133‑1 (MQ=255)
                                                                                                                    aGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGCCCGGCCCCCAacac                           >  4:326502/1‑133 (MQ=255)
                                                                                                                       tCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGCCCGGCCCCCAACACCCTGTGCTCGCCGGTGgcc        >  2:80949/1‑149 (MQ=255)
                                                                                                                          cACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGCCCGGCCCCCAACACCCTGTGCTCGCCGGTGgccgcc     <  4:38024/149‑1 (MQ=255)
                                                                                                                             cgcccgccAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGCCCGGCCCCCAACACCCTGTGCTCGCCGGTGGCCGCCCTg  >  7:107154/1‑149 (MQ=255)
                                                                                                                              |                                                                                                                                                   
CCGAGGCCGCGCTGGCAAAAGGCGTATCCGACGGGTTGCCTGATATTGAATCGGGTTGGTCGAAAGTGTGTGTGTGGGCTTTGGGTCATGTACAAGCGCATCGACCACAGATCGTCAGATCCCACCGCCCGCCAGCAACTGCGTCATCACCTCAGCCAGCGCCTTGACCATCACATCCGCCGTCGGCCGGTGCACGATGACGATCTCATAGCTGTCGACCTCCGGCAGCCCTTGCCCGGCCCCCAACACCCTGTGCTCGCCGGTGGCCGCCCTG  >  NC_002947/4005045‑4005318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑