Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,050,680 T→C 7.7% V238A (GTG→GCG) ‡ oprB‑III → carbohydrate‑selective porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,050,6800TC 7.7% 182.2 / 3.3 64V238A (GTG→GCG) ‡oprB‑IIIcarbohydrate‑selective porin
Reads supporting (aligned to +/- strand):  ref base T (28/31);  new base C (2/3);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCCAACGCCCACCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCATTACCTGCTGGT  >  NC_002947/4050534‑4050821
                                                                                                                                                   |                                                                                                                                             
ccccAACGCCCACCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTgg                                                                                                                                             >  3:127534/1‑149 (MQ=255)
 cccAACGCCCACCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGt                                                                                                                                            <  6:208484/149‑1 (MQ=255)
   cAACGCCCACCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTAc                                                                                                                                          >  6:25492/1‑149 (MQ=255)
       gCCCACCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGTAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGTACCACGGTGCCGGt                                                                                                                                      <  1:109947/149‑1 (MQ=255)
        cccACCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGTACCACGGTGCCGGtg                                                                                                                                     <  3:157419/149‑2 (MQ=255)
           aCCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTCGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCACCTCCAGCCGCGACT‑GGCACCTGGGGCCCAAGCACACCACCAGCACCGTGCTACCGGTAGaa                                                                                                                                  >  4:17850/1‑149 (MQ=255)
              tCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTg                                                                                                                               >  8:13767/1‑149 (MQ=255)
                ggCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTgg                                                                                                                              <  5:25492/148‑1 (MQ=255)
                     gcgcGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCATCTCCAGCCGCGCCTCGGCACCTGGGGCCCAAGCACACCACCGGTACCACGGTGCCGGtggtgt                                                                                                                                 <  1:271424/141‑6 (MQ=255)
                     gcgcGTGAAGTACGACCTGTCACCTGCCTCGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCAGAAAGCAACGGCAACTCCAGCCGCGCCT‑CGCACCTGGGGCCCAAGCACACCACCGGTACCACGGTGCCGGtggtgt                                                                                                                                 >  2:271424/1‑135 (MQ=255)
                       gcgTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAg                                                                                                                      <  5:287593/149‑1 (MQ=255)
                         gTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCt                                                                                                                    <  7:13767/149‑1 (MQ=255)
                            aaGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGc                                                                                                                  <  4:127534/148‑1 (MQ=255)
                            aaGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGc                                                                                                                  <  6:43902/148‑1 (MQ=255)
                                 cGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGt                                                                                                             <  2:308517/148‑1 (MQ=255)
                                    ccTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAAc                                                                                                         >  6:370268/1‑149 (MQ=255)
                                     cTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGt                                                                                                             >  1:8435/1‑144 (MQ=255)
                                     cTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGt                                                                                                             <  2:8435/144‑1 (MQ=255)
                                      tGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTg                                                                                                       >  6:191908/1‑149 (MQ=255)
                                        tccacggcctgTCAGTTGCGCGTGGCGGCGTTCCACGTCGACCCCGACAGCAACGGCAACTCCAGCCGCGCCT‑GACACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACGGGTAGAACTGGTATACAAGCTGCAGGTTGaa                                                                                                          <  3:238932/138‑1 (MQ=255)
                                        tCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GCCACCTGGGGCCCCAGCACACCACCGTCACCCTGGTACCGCTAGACCTGGAATACAAGCTGCAGGGTGaa                                                                                                          >  4:238932/1‑144 (MQ=255)
                                          aCCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAg                                                                                                   >  5:178524/1‑149 (MQ=255)
                                            ctgcctgGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGc                                                                                                 <  3:304990/149‑1 (MQ=255)
                                            ctgcctgGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGc                                                                                                 <  6:178524/149‑1 (MQ=255)
                                              gcctgGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGc                                                                                                 >  4:292834/1‑147 (MQ=255)
                                               cctgGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGa                                                                                               <  3:46150/148‑1 (MQ=255)
                                                ctgGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAg                                                                                              >  4:240893/1‑148 (MQ=255)
                                                      gTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGc                                                                                       <  5:370268/149‑1 (MQ=255)
                                                            cgTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGtt                                                                                 <  5:191908/149‑1 (MQ=255)
                                                                gcggcgTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTtacta                                                                             >  6:304901/1‑149 (MQ=255)
                                                                    cgTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTtactactac                                                                         >  1:208965/1‑149 (MQ=255)
                                                                           cGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGct                                                                  <  7:155209/149‑1 (MQ=255)
                                                                                aCCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGAt                                                             <  2:208965/149‑1 (MQ=255)
                                                                                   ccGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGAtgt                                                           >  5:107988/1‑148 (MQ=255)
                                                                                    cGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGAtgtg                                                          >  2:314401/1‑148 (MQ=255)
                                                                                            aCGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTtactactac                                                                         >  5:287234/1‑125 (MQ=255)
                                                                                            aCGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTtactactac                                                                         <  6:287234/125‑1 (MQ=255)
                                                                                               gCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGctc                                                                 <  1:145768/130‑1 (MQ=255)
                                                                                                 aaCTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAg                                            >  1:147254/1‑149 (MQ=255)
                                                                                                      cAGCCGCTCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCgat                                        <  3:240893/148‑1 (MQ=255)
                                                                                                         ccGCGCGT‑GGCACCTGGGGCCCAAGCACACCACCAGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGatgatg                                    <  3:17850/149‑1 (MQ=255)
                                                                                                         ccGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGatgatg                                    <  6:130893/149‑1 (MQ=255)
                                                                                                                   gCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTc                             <  3:292834/147‑1 (MQ=255)
                                                                                                                             cccAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCgatg                                       <  4:321541/127‑1 (MQ=255)
                                                                                                                             cccAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCgatg                                       <  4:316167/127‑1 (MQ=255)
                                                                                                                             cccAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCgatg                                       >  3:321541/1‑127 (MQ=255)
                                                                                                                             cccAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCgatg                                       >  3:316167/1‑127 (MQ=255)
                                                                                                                              ccAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGc                >  3:247953/1‑149 (MQ=255)
                                                                                                                               cAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAGACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGc                >  7:157212/1‑148 (MQ=255)
                                                                                                                                  gCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCAt             >  8:265261/1‑148 (MQ=255)
                                                                                                                                  cagttctaccGGTACCACGGTGCCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCAt             >  1:122222/8‑148 (MQ=255)
                                                                                                                                    acacCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCga                                         <  7:226621/118‑1 (MQ=255)
                                                                                                                                    acacCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCga                                         >  8:226621/1‑118 (MQ=255)
                                                                                                                                      acctccGGCACCGTGGTACCGGTAGATCTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAg                                 <  2:202884/124‑1 (MQ=255)
                                                                                                                                      accaccGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACACGCTTGGTTACTACTACGACAGCTCTGATGTGAAACCCATCGGCAGCGATGATGAAGTGTCCGGACGAGGTGGCCATTACct        >  5:200230/1‑149 (MQ=255)
                                                                                                                                      accaccGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAg                                 <  2:202888/124‑1 (MQ=255)
                                                                                                                                      accaccGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAg                                 >  1:202888/1‑124 (MQ=255)
                                                                                                                                      accaccGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGCGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACCACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAg                                 >  1:202884/1‑124 (MQ=255)
                                                                                                                                        caccGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAgt                                <  6:195797/123‑1 (MQ=255)
                                                                                                                                        caccGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAgt                                >  5:195797/1‑123 (MQ=255)
                                                                                                                                           cGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCATTACCTGCTgg   <  7:265261/149‑1 (MQ=255)
                                                                                                                                            ggCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCATTACCTGCTGGt  <  5:304901/149‑1 (MQ=255)
                                                                                                                                                ccGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGa                                                              >  2:127426/1‑85 (MQ=255)
                                                                                                                                                ccGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGa                                                              <  1:127426/85‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
CCCCAACGCCCACCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCATTACCTGCTGGT  >  NC_002947/4050534‑4050821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑