Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,050,684 A→G 7.9% V239V (GTA→GTG)  oprB‑III → carbohydrate‑selective porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,050,6840AG 7.9% 176.4 / 4.2 63V239V (GTA→GTG) oprB‑IIIcarbohydrate‑selective porin
Reads supporting (aligned to +/- strand):  ref base A (28/30);  new base G (2/3);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

CAACGCCCACCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCATTACCTGCTGGTCGACCAGGC  >  NC_002947/4050537‑4050830
                                                                                                                                                    |                                                                                                                                                  
cAACGCCCACCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTAc                                                                                                                                                   >  6:25492/1‑149 (MQ=255)
    gCCCACCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGTAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGTACCACGGTGCCGGt                                                                                                                                               <  1:109947/149‑1 (MQ=255)
     cccACCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGTACCACGGTGCCGGtg                                                                                                                                              <  3:157419/149‑2 (MQ=255)
        aCCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTCGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCACCTCCAGCCGCGACT‑GGCACCTGGGGCCCAAGCACACCACCAGCACCGTGCTACCGGTAGaa                                                                                                                                           >  4:17850/1‑149 (MQ=255)
           tCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTg                                                                                                                                        >  8:13767/1‑149 (MQ=255)
             ggCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTgg                                                                                                                                       <  5:25492/148‑1 (MQ=255)
                  gcgcGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCATCTCCAGCCGCGCCTCGGCACCTGGGGCCCAAGCACACCACCGGTACCACGGTGCCGGtggtgt                                                                                                                                          <  1:271424/141‑6 (MQ=255)
                  gcgcGTGAAGTACGACCTGTCACCTGCCTCGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCAGAAAGCAACGGCAACTCCAGCCGCGCCT‑CGCACCTGGGGCCCAAGCACACCACCGGTACCACGGTGCCGGtggtgt                                                                                                                                          >  2:271424/1‑135 (MQ=255)
                    gcgTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAg                                                                                                                               <  5:287593/149‑1 (MQ=255)
                      gTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCt                                                                                                                             <  7:13767/149‑1 (MQ=255)
                         aaGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGc                                                                                                                           <  6:43902/148‑1 (MQ=255)
                         aaGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGc                                                                                                                           <  4:127534/148‑1 (MQ=255)
                              cGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGt                                                                                                                      <  2:308517/148‑1 (MQ=255)
                                 ccTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAAc                                                                                                                  >  6:370268/1‑149 (MQ=255)
                                  cTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGt                                                                                                                      >  1:8435/1‑144 (MQ=255)
                                  cTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGt                                                                                                                      <  2:8435/144‑1 (MQ=255)
                                   tGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTg                                                                                                                >  6:191908/1‑149 (MQ=255)
                                     tccacggcctgTCAGTTGCGCGTGGCGGCGTTCCACGTCGACCCCGACAGCAACGGCAACTCCAGCCGCGCCT‑GACACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACGGGTAGAACTGGTATACAAGCTGCAGGTTGaa                                                                                                                   <  3:238932/138‑1 (MQ=255)
                                     tCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GCCACCTGGGGCCCCAGCACACCACCGTCACCCTGGTACCGCTAGACCTGGAATACAAGCTGCAGGGTGaa                                                                                                                   >  4:238932/1‑144 (MQ=255)
                                       aCCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAg                                                                                                            >  5:178524/1‑149 (MQ=255)
                                         ctgcctgGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGc                                                                                                          <  6:178524/149‑1 (MQ=255)
                                         ctgcctgGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGc                                                                                                          <  3:304990/149‑1 (MQ=255)
                                           gcctgGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGc                                                                                                          >  4:292834/1‑147 (MQ=255)
                                            cctgGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGa                                                                                                        <  3:46150/148‑1 (MQ=255)
                                             ctgGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAg                                                                                                       >  4:240893/1‑148 (MQ=255)
                                                   gTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGc                                                                                                <  5:370268/149‑1 (MQ=255)
                                                         cgTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGtt                                                                                          <  5:191908/149‑1 (MQ=255)
                                                             gcggcgTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTtacta                                                                                      >  6:304901/1‑149 (MQ=255)
                                                                 cgTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTtactactac                                                                                  >  1:208965/1‑149 (MQ=255)
                                                                        cGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGct                                                                           <  7:155209/149‑1 (MQ=255)
                                                                             aCCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGAt                                                                      <  2:208965/149‑1 (MQ=255)
                                                                                ccGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGAtgt                                                                    >  5:107988/1‑148 (MQ=255)
                                                                                 cGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGAtgtg                                                                   >  2:314401/1‑148 (MQ=255)
                                                                                         aCGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTtactactac                                                                                  >  5:287234/1‑125 (MQ=255)
                                                                                         aCGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTtactactac                                                                                  <  6:287234/125‑1 (MQ=255)
                                                                                            gCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGctc                                                                          <  1:145768/130‑1 (MQ=255)
                                                                                              aaCTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAg                                                     >  1:147254/1‑149 (MQ=255)
                                                                                                   cAGCCGCTCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCgat                                                 <  3:240893/148‑1 (MQ=255)
                                                                                                      ccGCGCGT‑GGCACCTGGGGCCCAAGCACACCACCAGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGatgatg                                             <  3:17850/149‑1 (MQ=255)
                                                                                                      ccGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGatgatg                                             <  6:130893/149‑1 (MQ=255)
                                                                                                                gCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTc                                      <  3:292834/147‑1 (MQ=255)
                                                                                                                          cccAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCgatg                                                <  4:321541/127‑1 (MQ=255)
                                                                                                                          cccAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCgatg                                                <  4:316167/127‑1 (MQ=255)
                                                                                                                          cccAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCgatg                                                >  3:316167/1‑127 (MQ=255)
                                                                                                                          cccAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCgatg                                                >  3:321541/1‑127 (MQ=255)
                                                                                                                           ccAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGc                         >  3:247953/1‑149 (MQ=255)
                                                                                                                            cAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAGACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGc                         >  7:157212/1‑148 (MQ=255)
                                                                                                                               gCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCAt                      >  8:265261/1‑148 (MQ=255)
                                                                                                                               cagttctaccGGTACCACGGTGCCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCAt                      >  1:122222/8‑148 (MQ=255)
                                                                                                                                 acacCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCga                                                  <  7:226621/118‑1 (MQ=255)
                                                                                                                                 acacCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCga                                                  >  8:226621/1‑118 (MQ=255)
                                                                                                                                   acctccGGCACCGTGGTACCGGTAGATCTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAg                                          <  2:202884/124‑1 (MQ=255)
                                                                                                                                   accaccGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACACGCTTGGTTACTACTACGACAGCTCTGATGTGAAACCCATCGGCAGCGATGATGAAGTGTCCGGACGAGGTGGCCATTACct                 >  5:200230/1‑149 (MQ=255)
                                                                                                                                   accaccGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAg                                          <  2:202888/124‑1 (MQ=255)
                                                                                                                                   accaccGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAg                                          >  1:202888/1‑124 (MQ=255)
                                                                                                                                   accaccGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGCGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACCACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAg                                          >  1:202884/1‑124 (MQ=255)
                                                                                                                                     caccGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAgt                                         <  6:195797/123‑1 (MQ=255)
                                                                                                                                     caccGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAgt                                         >  5:195797/1‑123 (MQ=255)
                                                                                                                                        cGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCATTACCTGCTgg            <  7:265261/149‑1 (MQ=255)
                                                                                                                                         ggCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCATTACCTGCTGGt           <  5:304901/149‑1 (MQ=255)
                                                                                                                                             ccGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGa                                                                       >  2:127426/1‑85 (MQ=255)
                                                                                                                                             ccGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGa                                                                       <  1:127426/85‑1 (MQ=255)
                                                                                                                                                  gTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCATTACCTGCTGGTCGACCAGGc  >  3:246873/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CAACGCCCACCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCT‑GGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCATTACCTGCTGGTCGACCAGGC  >  NC_002947/4050537‑4050830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑