Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,142,562 C→G 6.8% A232G (GCC→GGC)  PP_3646 → aldehyde dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,142,5620CG 6.8% 238.6 / 6.1 73A232G (GCC→GGC) PP_3646aldehyde dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base C (37/31);  new base G (2/3);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.77e-01

GGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATG  >  NC_002947/4142416‑4142705
                                                                                                                                                   |                                                                                                                                                  
ggCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTgcc                                                                                                                                                   <  3:341757/148‑1 (MQ=255)
    aCCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCGGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCAc                                                                                                                                              >  1:335405/1‑149 (MQ=255)
      cATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTgcc                                                                                                                                                   <  3:256129/142‑1 (MQ=255)
      cATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTgcc                                                                                                                                                   >  4:256129/1‑142 (MQ=255)
           tCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCAccgcccgc                                                                                                                                       >  8:57416/1‑149 (MQ=255)
              aGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGGCGGCAccgccggtgaa                                                                                                                                    <  5:75297/149‑7 (MQ=255)
               gCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGGCGGCAccgccggtgaag                                                                                                                                   <  6:222566/149‑8 (MQ=255)
                   gCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTgg                                                                                                                                >  4:159485/1‑148 (MQ=255)
                   gCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGt                                                                                                                               <  8:336873/149‑1 (MQ=255)
                    cccGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTa                                                                                                                              >  8:304615/1‑149 (MQ=255)
                        aCTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAATGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGt                                                                                                                               <  2:351414/144‑1 (MQ=255)
                        aCTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAATGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGt                                                                                                                               >  1:351414/1‑144 (MQ=255)
                          tGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACgcag                                                                                                                         >  6:306172/1‑148 (MQ=255)
                               cTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑gcgc                                                                                                                >  3:110536/1‑149 (MQ=255)
                                         gCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGACTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCagca‑‑‑gc                                                                                                                  >  1:235990/1‑138 (MQ=255)
                                         gCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCagca‑‑‑gc                                                                                                                  <  2:235990/137‑1 (MQ=255)
                                         gCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTc                                                                                                      <  4:105151/149‑1 (MQ=255)
                                         gCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTc                                                                                                      <  3:4744/149‑1 (MQ=255)
                                              ccggccggTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCaa                                                                                                 <  4:350592/149‑1 (MQ=255)
                                               cggccggTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAg                                                                                                >  1:157089/1‑149 (MQ=255)
                                                             aaCGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGGCGGCAccgccggt                                                                                                                                       <  2:211228/99‑4 (MQ=255)
                                                             aaCGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGGCGGCAccgccggt                                                                                                                                       >  1:211228/1‑96 (MQ=255)
                                                             aaCGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACt                                                                                  >  6:248995/1‑149 (MQ=255)
                                                             aaCGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACt                                                                                  >  5:203624/1‑149 (MQ=255)
                                                                 tGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGggc                                                                              <  1:59155/149‑1 (MQ=255)
                                                                     cACCGA‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGggcggc                                                                           >  8:138515/1‑148 (MQ=255)
                                                                      aCCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGggc                                                                              >  8:218471/1‑144 (MQ=255)
                                                                      aCCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGggc                                                                              <  7:218471/144‑1 (MQ=255)
                                                                       ccGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAg                                                                        <  7:27586/149‑1 (MQ=255)
                                                                        cGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGt                                                                       >  1:245553/1‑149 (MQ=255)
                                                                        cGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGt                                                                       >  7:281969/1‑149 (MQ=255)
                                                                                ggCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGaa                                                                >  8:322381/1‑149 (MQ=255)
                                                                                ggCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGaa                                                                >  5:368995/1‑149 (MQ=255)
                                                                                  ccccAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAACTGGGGCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCg                                                                  >  1:303574/1‑148 (MQ=255)
                                                                                  ccccAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAAca                                                              <  7:57416/149‑1 (MQ=255)
                                                                                   cccAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAAcat                                                             >  3:316352/1‑149 (MQ=255)
                                                                                    ccAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAAcat                                                             >  3:9764/1‑148 (MQ=255)
                                                                                     cAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTg                                                                                       >  8:204121/1‑121 (MQ=255)
                                                                                     cAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTg                                                                                       <  7:204121/121‑1 (MQ=255)
                                                                                      aGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAAcatcat                                                          <  5:306172/149‑1 (MQ=255)
                                                                                       gCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACatcatc                                                         <  7:138515/149‑1 (MQ=255)
                                                                                             gcgcggcgcTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTcgcc                                                   <  2:335405/149‑1 (MQ=255)
                                                                                                    gcTGATCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTcgccgacgccg                                            >  4:196272/1‑149 (MQ=255)
                                                                                                     cTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTcgccgacgccga                                           <  4:316352/149‑1 (MQ=255)
                                                                                                        aCCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCgccgacgccgac                                          <  4:110536/147‑1 (MQ=255)
                                                                                                           cGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGa                                     >  6:251635/1‑149 (MQ=255)
                                                                                                           cGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGa                                     <  2:157089/149‑1 (MQ=255)
                                                                                                             ccACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGa                                     >  1:298452/1‑147 (MQ=255)
                                                                                                             ccACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACa                                   <  7:304615/149‑1 (MQ=255)
                                                                                                              cACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAg                                  >  6:56134/1‑149 (MQ=255)
                                                                                                               aCCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAgc                                 >  2:208476/1‑149 (MQ=255)
                                                                                                                   gCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCa                             >  5:263737/1‑149 (MQ=255)
                                                                                                                    cTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCAt                            <  7:322381/149‑1 (MQ=255)
                                                                                                                       gTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATc                           <  1:208476/147‑1 (MQ=255)
                                                                                                                          cgcAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATg                       <  6:263737/148‑1 (MQ=255)
                                                                                                                           gcAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGgc                     >  8:136557/1‑149 (MQ=255)
                                                                                                                           gcAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGgc                     >  6:367631/1‑149 (MQ=255)
                                                                                                                           gcAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGgc                     <  6:203624/149‑1 (MQ=255)
                                                                                                                             aaGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAAt                        >  6:339274/1‑144 (MQ=255)
                                                                                                                             aaGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAAt                        <  5:339274/144‑1 (MQ=255)
                                                                                                                                aTCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGt                >  5:104484/1‑149 (MQ=255)
                                                                                                                                   gCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGc             <  5:248995/149‑1 (MQ=255)
                                                                                                                                   gCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGc             >  3:87912/1‑149 (MQ=255)
                                                                                                                                    ccTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGcc            <  4:9764/149‑1 (MQ=255)
                                                                                                                                    ccTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGcc            >  6:358654/1‑149 (MQ=255)
                                                                                                                                    ccTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGcc            <  2:298452/149‑1 (MQ=255)
                                                                                                                                    ccTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAACCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGcc            >  6:363859/1‑149 (MQ=255)
                                                                                                                                     cTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCg           >  1:328252/1‑149 (MQ=255)
                                                                                                                                        ggcgggcggTGGCGGCACCGCCGGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCa        >  5:24857/5‑149 (MQ=255)
                                                                                                                                          ccGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATc      <  5:251635/149‑1 (MQ=255)
                                                                                                                                          ccGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATc      <  2:245553/149‑1 (MQ=255)
                                                                                                                                           cggcggTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATc      >  4:9701/1‑148 (MQ=255)
                                                                                                                                           cggcggTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATc      >  3:98870/1‑148 (MQ=255)
                                                                                                                                           cggcggTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCt     <  2:303574/149‑1 (MQ=255)
                                                                                                                                             gcggTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTAt   <  3:159485/149‑1 (MQ=255)
                                                                                                                                              cggTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATg  <  5:367631/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGG‑CTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCA‑‑‑GCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATG  >  NC_002947/4142416‑4142705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑