Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,188,349	G→T	10.3%	R82L (CGT→CTT) ‡	PP_3684 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,188,349	0	G	T	10.3%	238.9 / 15.3	85	R82L (CGT→CTT) ‡	PP_3684	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (40/36);  new base T (3/6);  total (43/42)
Fisher's exact test for biased strand distribution p-value = 3.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.86e-01

GTCTTCCCAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGTATAG  >  NC_002947/4188201‑4188496                                                                                                                                                     |                                                                                                                                                    gTCTTCCCAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGAACGACCATGAAGAGATACGTGATGTGATCCTTGAGAGCCg                                                                                                                                                     >  6:68594/1‑149 (MQ=255)       ccAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGc                                                                                                                                               >  4:175225/1‑149 (MQ=255)             cGAAGACCAGAAGCAGAGATAAGGCATGAGCACCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCTCACGGCTCTCaa                                                                                                                                         <  3:57188/149‑1 (MQ=255)              gAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCTCACGGCTCTCAAg                                                                                                                                        <  1:65245/149‑1 (MQ=255)                 gACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCTCACGGCTCTCAAggat                                                                                                                                     <  2:10208/149‑4 (MQ=255)                 gACAAGAAGCAGAGATAAGTTATGAGATCCAATTCATCTTGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCTCACGGCTCTCAAggat                                                                                                                                     <  6:287895/149‑4 (MQ=255)                    cAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACt                                                                                                                                    <  3:115802/147‑1 (MQ=255)                    cAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACt                                                                                                                                    >  4:115802/1‑147 (MQ=255)                    cAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTg                                                                                                                                  >  7:167515/1‑149 (MQ=255)                     aGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTgg                                                                                                                                 <  5:265679/149‑1 (MQ=255)                        aGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGATCAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTc                                                                                                                               <  2:190665/148‑1 (MQ=255)                         gCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGa                                                                                                                             >  2:20047/1‑149 (MQ=255)                            gagaTAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGAccc                                                                                                                          <  8:239751/149‑1 (MQ=255)                                    gCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATc                                                                                                                  >  5:310535/1‑149 (MQ=255)                                    gCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATc                                                                                                                  >  1:341967/1‑149 (MQ=255)                                    gCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATc                                                                                                                  <  6:88095/149‑1 (MQ=255)                                    gCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACATTGAGGGACACTGACCGAGGCGGGATATCGATCGACCCTGAAGAGATACGTTATGTGATCCTTGAGAGCCGTCAGGCGCACAAGCACATGGACGCCCCGgccaaac                                                                                                                  >  2:332011/1‑144 (MQ=255)                                         aGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGaaa                                                                                                             <  4:203995/149‑1 (MQ=255)                                         aGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGaaa                                                                                                             <  8:220382/149‑1 (MQ=255)                                           tgcCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGc                                                                                                           <  7:341145/148‑1 (MQ=255)                                           cgcCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCTCACGGCTCTCAAg                                                                                                                                        >  5:373657/1‑120 (MQ=255)                                           cgcCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCTCACGGCTCTCAAg                                                                                                                                        <  6:373657/120‑1 (MQ=255)                                            gcCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGc                                                                                                           >  4:185524/1‑148 (MQ=255)                                            gcCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGc                                                                                                           >  3:134858/1‑148 (MQ=255)                                             ccAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGc                                                                                                           >  8:341145/1‑147 (MQ=255)                                              cAATTCATCGAGGCGGTTAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGGGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGaa                                                                                                        <  7:270710/149‑1 (MQ=255)                                              cAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGaa                                                                                                        >  7:102834/1‑149 (MQ=255)                                              cAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGaa                                                                                                        <  5:289358/149‑1 (MQ=255)                                                  gttttgAGGCTGTTCACCGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCTGGCGCATCGGAAAGCGAACaa                                                                                                     >  3:209025/6‑148 (MQ=255)                                                    aTCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACaaaa                                                                                                   >  4:289987/1‑148 (MQ=255)                                                        aGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCt                                                                                                >  1:363500/1‑147 (MQ=255)                                                        aGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGa                                                                                              <  6:282201/149‑1 (MQ=255)                                                          gCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAgc                                                                                            >  5:18275/1‑149 (MQ=255)                                                                aaCAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCgctgc                                                                                       <  3:147425/148‑1 (MQ=255)                                                                     ccTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTgg                                                                                 >  2:228316/1‑149 (MQ=255)                                                                       tCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGtt                                                                               >  3:159235/1‑149 (MQ=255)                                                                       tCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGtt                                                                               >  4:223784/1‑149 (MQ=255)                                                                         aaaaCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGttt                                                                              <  1:20047/148‑1 (MQ=255)                                                                          aaaCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCa                                                                            <  4:134858/149‑1 (MQ=255)                                                                           aaCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAg                                                                           <  2:363500/149‑1 (MQ=255)                                                                           aaCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAg                                                                           <  6:178781/149‑1 (MQ=255)                                                                              cTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGAtc                                                                        >  3:65849/1‑149 (MQ=255)                                                                               ttGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGAtct                                                                       <  3:289987/149‑1 (MQ=255)                                                                                      tCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGcc                                                                 >  4:298267/1‑148 (MQ=255)                                                                                          tGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCctct                                                             >  6:340340/1‑148 (MQ=255)                                                                                             ccGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTaaa                                                          <  8:167515/148‑1 (MQ=255)                                                                                                  gCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCATGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAg                                                    <  2:341967/149‑1 (MQ=255)                                                                                                     ggATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGa                                                 >  1:241551/1‑149 (MQ=255)                                                                                                     ggATGTCGATCGACCCTGAAGAGATACGTGATGTGATACTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGa                                                 >  3:351925/1‑149 (MQ=255)                                                                                                        tGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACtt                                              <  1:332011/149‑1 (MQ=255)                                                                                                        tGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACtt                                              <  3:223784/149‑1 (MQ=255)                                                                                                         gTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGc                                                      >  1:330893/1‑140 (MQ=255)                                                                                                         gTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGc                                                      <  2:330893/140‑1 (MQ=255)                                                                                                            gatcgaCCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCaa                                           <  8:160475/148‑1 (MQ=255)                                                                                                            gatcgaCCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCaa                                           <  8:102834/148‑1 (MQ=255)                                                                                                            gatcgaCCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCaa                                           >  7:160475/1‑148 (MQ=255)                                                                                                                gaCCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCg                                      <  4:65849/149‑1 (MQ=255)                                                                                                                 aCCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCACTCTAAACTGCAGCGACTTCAACTGCg                                      >  1:296407/1‑148 (MQ=255)                                                                                                                     tGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTAttt                                 >  2:19344/1‑149 (MQ=255)                                                                                                                      gAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCa                                                     >  2:16304/1‑128 (MQ=255)                                                                                                                        agagATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCct                                                               <  8:338272/116‑1 (MQ=255)                                                                                                                        agagATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCct                                                               >  7:338272/1‑116 (MQ=255)                                                                                                                          agaTACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTtgtga                            >  3:175162/1‑148 (MQ=255)                                                                                                                           gaTACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCTGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGcc                           <  4:209025/149‑1 (MQ=255)                                                                                                                            aTACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGccc                          >  3:133426/1‑149 (MQ=255)                                                                                                                                  gatgtgatCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGAGCATCGGAAAGCGAACAAAACATGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCttt                       >  5:177710/1‑146 (MQ=255)                                                                                                                                       gatCCTTGAGAGCCGTGAGGCTCTCAAGGACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGa               >  1:209060/1‑149 (MQ=255)                                                                                                                                       gatCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGa               >  1:67303/1‑149 (MQ=255)                                                                                                                                        atCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGa               <  3:298267/148‑1 (MQ=255)                                                                                                                                        atCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGAc              <  2:241551/149‑1 (MQ=255)                                                                                                                                          ccTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTCGTGCCCTTTGCGCTCGACaa            >  5:9285/1‑149 (MQ=255)                                                                                                                                            ttGAGAGCCTCACGGCTCTCAAGCACTTGGTCTACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAAGAGCGGATTTTGTGCCCTTTGCGCTCGACAACt          >  2:116746/1‑149 (MQ=255)                                                                                                                                            ttGAGAGCCTCACGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACt          >  8:101583/1‑149 (MQ=255)                                                                                                                                            ttGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTa                                    <  5:238780/123‑1 (MQ=255)                                                                                                                                            ttGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTa                                    >  6:238780/1‑123 (MQ=255)                                                                                                                                            ttGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACt          >  2:172681/1‑149 (MQ=255)                                                                                                                                            ttGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACt          >  3:307211/1‑149 (MQ=255)                                                                                                                                              gagaGCCTTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTc         <  5:68594/148‑1 (MQ=255)                                                                                                                                              gagaGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTcc        >  2:365549/1‑149 (MQ=255)                                                                                                                                              gagaGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTc         <  2:67303/148‑1 (MQ=255)                                                                                                                                               agagCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTcc        <  1:365549/148‑1 (MQ=255)                                                                                                                                               agagCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTc         <  2:209060/147‑1 (MQ=255)                                                                                                                                               agagCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGAATTTTGTGCCCTTTGCGCTCGACAACTcc        <  6:310535/148‑1 (MQ=255)                                                                                                                                                  gCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGtat    <  3:175225/149‑1 (MQ=255)                                                                                                                                                    cGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGta     >  7:97123/1‑146 (MQ=255)                                                                                                                                                    cGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGTATAg  <  3:185524/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GTCTTCCCAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGTATAG  >  NC_002947/4188201‑4188496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑