Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,277,201	T→C	6.8%	S199S (AGT→AGC)	glcF →	glycolate oxidase iron‑sulfur subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,277,201	0	T	C	6.8%	166.5 / 2.9	59	S199S (AGT→AGC)	glcF	glycolate oxidase iron‑sulfur subunit
Reads supporting (aligned to +/- strand):  ref base T (28/27);  new base C (2/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

AGTTGCCGGCGCAAGTCACCACACCGGGCCAGCGTCCGGCAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGACGCCTGGTGGCCCGCCATCGAAGCGGGTGTCGAGGCTATCGTGCAGACCGC  >  NC_002947/4277053‑4277347                                                                                                                                                     |                                                                                                                                                     aGTTGCCGGCGCAAGTCACCACACCGGGCCAGCGTCCGGCAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAgt                                                                                                                                                      <  4:266785/149‑1 (MQ=255)   ttGCCGGCGCAAGTCACCACACCGGGCCAGCGTCCGGCAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAgtg‑‑t                                                                                                                                                  <  2:288552/149‑1 (MQ=255)   ttGCCGGCGCAAGTCACCACACCGGGCCAGCGTCCGGCAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAgtg‑‑t                                                                                                                                                  <  2:165507/149‑1 (MQ=255)   ttGCCGGCGCAAGTCACCACACCGGGCCAGCGTCCGGCAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAgtg‑‑t                                                                                                                                                  <  5:141711/149‑1 (MQ=255)        ggCGCAAGTCACCACACCGGGCCAGCGTCCGGCAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCaa                                                                                                                                              >  6:310668/1‑148 (MQ=255)        ggCGCAAGTCACCACACCGGGCCAGCGTCCGGCAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCaa                                                                                                                                              <  5:310668/148‑1 (MQ=255)                tCACCACACCGGGCCAGCGTCCGGCAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCgg                                                                                                                                     >  5:297539/1‑149 (MQ=255)                     acacCGGGCCAGCGTCCGGCAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGc                                                                                                                                >  4:99899/1‑149 (MQ=255)                       acCGGGCCAGCGTCCGGCAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCg                                                                                                                              >  4:289201/1‑149 (MQ=255)                          gggCCAGGGTCCGGCAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGAt                                                                                                                           <  3:299816/149‑1 (MQ=255)                           ggCCAGCGTCCGGCAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATg                                                                                                                          <  8:83976/149‑1 (MQ=255)                             ccAGCGTCCGGCAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCt                                                                                                                        <  3:35823/149‑1 (MQ=255)                             ccAGCGTCCGGCAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCt                                                                                                                        <  3:31163/149‑1 (MQ=255)                              cAGCGTCCGGCAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCtg                                                                                                                       <  6:297539/149‑1 (MQ=255)                                 cGTCCGGTAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTgg                                                                                                                    >  2:368543/1‑149 (MQ=255)                                   tCCGGCAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGgcg                                                                                                                  >  7:170599/1‑149 (MQ=255)                                       gCAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGt                                                                                                              <  1:108675/149‑1 (MQ=255)                                       gCAGTGAGCAATGCACGCCGGATACTGATGCTGGACGGCTGCGTGCAGCCGGCCCTGTCCCCGATTACCAATGCCGCTGCTGCGCGGCTGCTCGACAGCCGGGGGAGCAGTG‑‑TGCACCCGGTCCGGCAGGCCGGATGCTGTGGCGCGGt                                                                                                              >  6:262282/1‑149 (MQ=255)                                          gTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGGTGCGCGGCTGCTCGACCGCCTGGGTTTCAGTG‑‑TGCAACCTGTCCGGGAGGCCGGATGCTGTGGCGCGGTGGa                                                                                                           <  5:106676/149‑1 (MQ=255)                                                    cACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTg                                                                                                 <  1:368543/149‑1 (MQ=255)                                                         cGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGc                                                                                            >  6:282238/1‑149 (MQ=255)                                                             tACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAg                                                                                        <  5:140484/149‑1 (MQ=255)                                                                   tGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTGTCTTCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGc                                                                                    <  1:106712/149‑1 (MQ=255)                                                                   tGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCaa                                                                                  <  5:282238/149‑1 (MQ=255)                                                                    gCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGCG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAg                                                                                 >  3:28502/1‑149 (MQ=255)                                                                    gCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGCG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAg                                                                                 >  1:113036/1‑149 (MQ=255)                                                                     cTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAg                                                                                 <  3:289201/148‑1 (MQ=255)                                                                       ggAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCAc                                                                                                    <  8:275357/127‑1 (MQ=255)                                                                       ggAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCAc                                                                                                    >  7:275357/1‑127 (MQ=255)                                                                                cGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCg                                                                       <  6:365651/147‑1 (MQ=255)                                                                                cGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCGGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGTACGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCgg                                                                      <  5:262282/148‑1 (MQ=255)                                                                                 gTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCggg                                                                     >  5:332031/1‑148 (MQ=255)                                                                                 acgCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTTGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCggg                                                                     >  4:108202/3‑148 (MQ=255)                                                                                  tGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCtg                                                                                                                       <  1:137069/97‑1 (MQ=255)                                                                                  tGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCtg                                                                                                                       >  2:137069/1‑97 (MQ=255)                                                                                         ggCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGgcgc                                                             >  2:224586/1‑148 (MQ=255)                                                                                            ccTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAAt                                                                                              >  5:95720/1‑112 (MQ=255)                                                                                            ccTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAAt                                                                                              <  6:95720/112‑1 (MQ=255)                                                                                            ccTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAata                                                         >  1:174068/1‑149 (MQ=255)                                                                                                  aCCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAg                                                                                        >  6:83545/1‑112 (MQ=255)                                                                                                  aCCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAg                                                                                        <  5:83545/112‑1 (MQ=255)                                                                                                  aCCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGCG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGAc                                                    <  2:113036/148‑1 (MQ=255)                                                                                                  aCCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGCG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGAc                                                    <  4:28502/148‑1 (MQ=255)                                                                                                     cAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCg                                                                       <  4:299676/126‑1 (MQ=255)                                                                                                     cAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCg                                                                       >  3:299676/1‑126 (MQ=255)                                                                                                        tACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGACGCCtggt                                             <  1:297894/149‑1 (MQ=255)                                                                                                         aCCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGACGCCtggtg                                            >  8:110975/1‑149 (MQ=255)                                                                                                          ccAATGCCGCTGCTGCGCGGCTGCTTGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGACGCCtggtg                                            <  3:108202/148‑1 (MQ=255)                                                                                                           cAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGACGCCTGGTGGc                                          >  1:269992/1‑149 (MQ=255)                                                                                                             aTGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGACGCCTGGTGGccc                                        >  4:97554/1‑149 (MQ=255)                                                                                                                ccGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCaa                                                           >  2:343933/1‑127 (MQ=255)                                                                                                                ccGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCaa                                                           <  1:343933/127‑1 (MQ=255)                                                                                                                     gctgcGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGACGCCTGGTGGCCCGCCATCg                                 >  6:379549/1‑148 (MQ=255)                                                                                                                      ctgcGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGACGCCTGGTGGCCCGCCATCGaa                               <  6:332031/149‑1 (MQ=255)                                                                                                                       tgcGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGACGCCTGGTGGCCCGCCATCGAAg                              >  5:356588/1‑149 (MQ=255)                                                                                                                         cgcgGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGACGCCTGGTGGCCCGCCATCGAAGc                             <  2:269992/148‑1 (MQ=255)                                                                                                                           cgGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGACGCCTGGTGGCCCGCCATCGAAGCggg                          >  4:31418/1‑149 (MQ=255)                                                                                                                             gctgctCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGACGCCTGGTGGCCCGCCATCGAAGCGGgtg                        >  5:312380/1‑149 (MQ=255)                                                                                                                                 ctCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGACGCCTGGTGGCCCGCCATCGAAGCGGGTGTc                      >  8:187671/1‑147 (MQ=255)                                                                                                                                            gggTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGACGCCTGGTGGCCCGCCATCGAAGCGGGTGTCGAGGCTATCGTg          <  5:278782/148‑1 (MQ=255)                                                                                                                                            gggTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGACGCCTGGTGGCCCGCCATCGAAGCGGGTGTCGAGGCTATCGTg          >  6:278782/1‑148 (MQ=255)                                                                                                                                              gTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGACGCCTGGTGGCCCGCCATCGAAGCGGGTGTCGAGGCTATCGTg          >  2:294577/1‑146 (MQ=255)                                                                                                                                                   aGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGACGCCTGGTGGCCCGCCATCGAAGCGGGTGTCGAGGCTATCGTGCAGACCGc  <  3:296477/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     AGTTGCCGGCGCAAGTCACCACACCGGGCCAGCGTCCGGCAGTGCGCCATGCACGCCGGATACTGATGCTGGAAGGCTGCGTGCAGCCGGCCCTGTCACCCAATACCAATGCCGCTGCTGCGCGGCTGCTCGACCGCCTGGGTATCAGTG‑‑TGCAACCGGTCCGGCAGGCCGGATGCTGTGGCGCGGTGGACTATCACCTGAATGCCCAGGAGCAAGGCCTGCAGCGGGCACGGCGCAATATCGACGCCTGGTGGCCCGCCATCGAAGCGGGTGTCGAGGCTATCGTGCAGACCGC  >  NC_002947/4277053‑4277347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑