Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,281,125 G→A 6.3% A100V (GCG→GTG)  PP_3751 ← cyanate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,281,1250GA 6.3% 189.5 / 2.0 63A100V (GCG→GTG) PP_3751cyanate transporter
Reads supporting (aligned to +/- strand):  ref base G (31/28);  new base A (2/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

GCCCGAGTACTGCAGCCAGGCCGCCGCCGCTCATCAGCGCCGCCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAG  >  NC_002947/4280979‑4281273
                                                                                                                                                  |                                                                                                                                                    
gCCCGAGTACTGCAGCCAGGCCGCCGCCGCTCATCAGCGCCGCCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAgcg                                                                                                                                                      >  5:176480/1‑147 (MQ=255)
gCCCGAGTACTGCAGCCAGGCCGCCGCCGCTCATCAGCGCCGCCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCa                                                                                                                                                    >  4:99973/1‑149 (MQ=255)
  ccgccgaCTGCACCCCTGCCGCCGCCGCTCATCAGCGCCGCCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACt                                                                                                                                                  <  4:368008/143‑1 (MQ=255)
     aGTACTGCAGCCAGGCCGCCGCCGCTCATCAGCGCCGCCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGc                                                                                                                                               <  5:318204/149‑1 (MQ=255)
         cTGCAGCCAGGCCGCCGCCGCTCATCAGCGCCGCCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATc                                                                                                                                           <  7:248277/149‑1 (MQ=255)
          tGCAGCCAGGCCGCCGCCGCTCATCAGCGCCGCCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGAt                                                                                                                                            >  3:51356/1‑147 (MQ=255)
          tGCAGCCAGGCCGCCGCCGCTCATCAGCGCCGCCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGAt                                                                                                                                            >  4:362672/1‑147 (MQ=255)
            cAGCCAGGCCGCCGCCGCTCATCAGCGCCGCCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTg                                                                                                                                                 >  5:312256/1‑140 (MQ=255)
            cAGCCAGGCCGCCGCCGCTCATCAGCGCCGCCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTg                                                                                                                                                 <  6:312256/140‑1 (MQ=255)
            cAGCCAGGCCGCCGCCGCTCATCAGCGCCGCCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAgc                                                                                                                                        <  7:60910/149‑1 (MQ=255)
             aGCCAGGCCGCCTCCGCTCATCAGCGCCGCCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCTGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCAGAACCACCGCCACGACCCGCCCAGCCACCAGCGCACGGGCGAt                                                                                                                                            >  2:274456/1‑144 (MQ=255)
             aGCCAGGCCGCCGCCGCTCATCAGCGCCTGCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGATGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGAt                                                                                                                                            <  1:274456/144‑1 (MQ=255)
                 aGGCCGCCGCCGCTCATCAGCGCCGCCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACAAGCCCCGGCATCATGCCCTGAGACCCCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGcc                                                                                                                                     >  4:278326/1‑147 (MQ=255)
                    ccgccgccgCTCATCAGCGCCGCCGAGTACCGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAgg                                                                                                                                 >  7:211107/1‑148 (MQ=255)
                     cgccgcggcTCATCAGCGCCGCCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCCGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCa                                                                                                                               >  1:169698/1‑149 (MQ=255)
                     cgccgccgcTCATCAGCGCCGCCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCa                                                                                                                               <  3:99973/149‑1 (MQ=255)
                            gcTCATCAGCGCCGCCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCACACTGGCGATCAGCGCCCAGGCACTGTc                                                                                                                          >  5:17032/1‑147 (MQ=255)
                              tcatcaGCGCCGCCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGc                                                                                                                      >  2:363004/1‑149 (MQ=255)
                                       ccgccgAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCACACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGgcgc                                                                                                             <  6:17032/149‑1 (MQ=255)
                                           cgAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCca                                                                                                           >  2:8271/1‑147 (MQ=255)
                                                    ccccATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTgg                                                                                                                                                <  2:368359/101‑1 (MQ=255)
                                                    ccccATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTgg                                                                                                                                                >  1:368359/1‑101 (MQ=255)
                                                    ccccATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCACACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCa                                                                                                >  2:350810/1‑149 (MQ=255)
                                                       cATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCcac                                                                                                          >  6:172900/1‑136 (MQ=255)
                                                       cATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCcac                                                                                                          <  5:172900/136‑1 (MQ=255)
                                                         tCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGc                                                                                            >  6:182256/1‑148 (MQ=255)
                                                               cGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCACACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATcgcg                                                                                     <  1:350810/149‑1 (MQ=255)
                                                                 gCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGc                                                                                   <  1:8271/149‑1 (MQ=255)
                                                                   cAGACGCCCAGGGAACCAGCGTTTGACCAGCCCCGGCATCATTCCCTGAACCACCGCCACGCCCAGTCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCa                                                                                 <  3:278326/149‑1 (MQ=255)
                                                                       cGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGcc                                                                             >  1:248963/1‑149 (MQ=255)
                                                                        gCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGccc                                                                            >  3:360344/1‑149 (MQ=255)
                                                                        gCCCAGGGAACCAGCGGGTGACCAGCCCCGTCATCATGCCCTGAACCTCCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGccc                                                                            <  2:97605/149‑1 (MQ=255)
                                                                          ccAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCa                                                                                                                                   >  3:324411/1‑92 (MQ=255)
                                                                          ccAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCa                                                                                                                                   <  4:324411/92‑1 (MQ=255)
                                                                           cAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGcccccc                                                                         <  4:360344/149‑1 (MQ=255)
                                                                           cAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGcccccc                                                                         <  3:362672/149‑1 (MQ=255)
                                                                             gggAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAg                                                                       >  5:57759/1‑149 (MQ=255)
                                                                                  ccAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAgg                                                                                              >  3:269742/1‑121 (MQ=255)
                                                                                  ccAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAgg                                                                                              <  4:269742/121‑1 (MQ=255)
                                                                                              cAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGc                                                      >  2:352875/1‑149 (MQ=255)
                                                                                              cAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGc                                                      >  2:347419/1‑149 (MQ=255)
                                                                                                   ccGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCa                                                             <  3:263542/137‑1 (MQ=255)
                                                                                                   ccGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCa                                                             >  4:263542/1‑137 (MQ=255)
                                                                                                   ccGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGAcc                                                 <  1:352875/149‑1 (MQ=255)
                                                                                                   ccGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGAcc                                                 <  1:347419/149‑1 (MQ=255)
                                                                                                    cGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGAccc                                                >  7:172338/1‑149 (MQ=255)
                                                                                                     ggCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCa                                               <  4:51356/149‑1 (MQ=255)
                                                                                                      gCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAg                                              <  4:174287/149‑1 (MQ=255)
                                                                                                       catcatGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCTGGCACTGTCCAGCTGCAGGCGCCACAGGCTGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGc                                             <  2:169698/149‑1 (MQ=255)
                                                                                                       catcatGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGc                                             >  3:114306/1‑149 (MQ=255)
                                                                                                               ccTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGCGTCGCAGc                                     >  4:354417/1‑149 (MQ=255)
                                                                                                               ccTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAAGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGCGTCGCAGc                                     >  6:223554/1‑149 (MQ=255)
                                                                                                                     ccaccGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGCGTCGCAGCCACGGt                               <  5:188519/149‑1 (MQ=255)
                                                                                                                       accGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGCGTCGCAGCCACGGTTg                             >  3:335450/1‑149 (MQ=255)
                                                                                                                              cGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGCGTCGCAGCCACGGTTGCAACAgg                      <  8:211107/149‑1 (MQ=255)
                                                                                                                               gCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAAGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGCGTCGCAGCCACGGTTGCAACAggg                     <  5:223554/149‑1 (MQ=255)
                                                                                                                                 ccAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGCGTCGCAGCCACGGTTGCAACAGGGGc                   >  2:124525/1‑149 (MQ=255)
                                                                                                                                  cAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGCGTCGCAGCCACGGTTGCAACAGGGGCa                  >  2:167981/1‑149 (MQ=255)
                                                                                                                                   aGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGCGTCGCAGCCACGGTTGCAACAgg                      >  5:269439/1‑144 (MQ=255)
                                                                                                                                   aGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGCGTCGCAGCCACGGTTGCAACAgg                      <  6:269439/144‑1 (MQ=255)
                                                                                                                                       cagccaCCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGCGTCGCAGCCACGGTTGCAACAGGGGCACCAgg             >  6:204894/1‑149 (MQ=255)
                                                                                                                                        agccaCCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGc            >  2:100540/1‑149 (MQ=255)
                                                                                                                                            accaGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCAt        <  4:335450/149‑1 (MQ=255)
                                                                                                                                               aGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGca     <  3:354417/149‑1 (MQ=255)
                                                                                                                                                  gcACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAg  <  4:114306/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCCCGAGTACTGCAGCCAGGCCGCCGCCGCTCATCAGCGCCGCCGAGTACAGCCCCATCGCCCCGGCCAGACGCCCAGGGAACCAGCGGTTGACCAGCCCCGGCATCATGCCCTGAACCACCGCCACGCCCAGGCCAGCCACCAGCGCACTGGCGATCAGCGCCCAGGCACTGTCCAGCTGCAGGCGCCACAGGCAGGCCAGGGCAATCGCGGCCAGGCCCCCCAGCATGCCGCCATGCTCGCTGACCCAGCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAG  >  NC_002947/4280979‑4281273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑