Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,301,575 G→C 11.3% intergenic (+65/‑1235) PP_3774 → / → PP_5627 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,301,5750GC11.3% 151.8 / 9.7 53intergenic (+65/‑1235)PP_3774/PP_5627hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/24);  new base C (3/3);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

ACCAGGTCGACTACACCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGATCTCTGCC  >  NC_002947/4301428‑4301722
                                                                                                                                                   |                                                                                                                                                   
aCCAGGTCGACTACACCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAgcgc                                                                                                                                                    >  7:226955/1‑149 (MQ=255)
    ggTCGACTACACCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCgggg                                                                                                                                                <  3:68156/149‑1 (MQ=255)
       cGACTACACCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGgggctg                                                                                                                                             <  8:281102/149‑1 (MQ=255)
          cTACACCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCgg                                                                                                                                                  >  4:174234/1‑141 (MQ=255)
          cTACACCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCgg                                                                                                                                                  <  3:174234/141‑1 (MQ=255)
            acacCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCtt                                                                                                                                        <  8:226955/149‑1 (MQ=255)
             cacCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCtt                                                                                                                                        <  2:226323/148‑1 (MQ=255)
             cacCGACGCAACCCCCGCCGAGGACGGCTCGAACTGCATCATCAGCGAGCTATCGCTCATACATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATCAATGCAAAAACCCCAGCGCCGGGCTGGCCTTc                                                                                                                                       >  6:358175/1‑149 (MQ=255)
              acCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTc                                                                                                                                       >  7:219989/1‑148 (MQ=255)
              acCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCt                                                                                                                                      <  5:81964/149‑1 (MQ=255)
              acCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCt                                                                                                                                      <  4:117304/149‑1 (MQ=255)
                 gACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCCCCGCGCTGGGCtttttgc                                                                                                                                   <  5:254940/149‑6 (MQ=255)
                       aCCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTgg                                                                                                                             <  2:209590/149‑1 (MQ=255)
                        cccccGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCCCCgcgctgggc                                                                                                                                          >  8:32416/1‑135 (MQ=255)
                        cccccGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCCCCgcgctgggc                                                                                                                                          <  7:32416/135‑1 (MQ=255)
                             gccgAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCACTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGa                                                                                                                        >  7:139249/1‑148 (MQ=255)
                                      ggCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTc                                                                                                              >  4:239805/1‑149 (MQ=255)
                                        cTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTGTCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCCCCGCGCTGGGCttttt                                                                                                                                     <  2:74789/124‑4 (MQ=255)
                                        cTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTGTCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCCCCGCGCTGGGCttttt                                                                                                                                     >  1:74789/1‑121 (MQ=255)
                                        cTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAg                                                                                                                                 >  3:240554/1‑128 (MQ=255)
                                        cTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAg                                                                                                                                 <  4:240554/128‑1 (MQ=255)
                                            aTCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAg                                                                                                        >  3:108912/1‑149 (MQ=255)
                                                 catcatCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCtt                                                                                                    >  6:1722/1‑148 (MQ=255)
                                                    catcaGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTAACAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTaca                                                                                                >  1:214595/1‑149 (MQ=255)
                                                      tcaGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTacaca                                                                                              >  3:211802/1‑149 (MQ=255)
                                                         gCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTTGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTg                                                                                           <  3:239805/149‑1 (MQ=255)
                                                             gCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACt                                                                                       <  8:219989/149‑1 (MQ=255)
                                                               tctcGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTg                                                                                      >  5:124459/1‑148 (MQ=255)
                                                                 tcGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGaa                                                                                   >  4:72904/1‑149 (MQ=255)
                                                                   gCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGt                                                                                 >  7:206479/1‑149 (MQ=255)
                                                                   gCTCATCCATCGCTGAACGTCGATGACCAGCCCTGCTATCTAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCGCAGCGCTGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGaa                                                                                   <  1:143101/147‑1 (MQ=255)
                                                                     tCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGtt                                                                                >  8:139750/1‑148 (MQ=255)
                                                                       atccatcGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTa                                                                               <  4:211802/147‑1 (MQ=255)
                                                                        tccatcACTGAACGTCGATGCCCAGCCCTGCTATCATAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTc                                                                            <  8:139249/149‑1 (MQ=255)
                                                                            tcGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGt                                                                        >  5:120217/1‑149 (MQ=255)
                                                                                 gAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGgcg                                                                   >  5:137773/1‑149 (MQ=255)
                                                                                    cGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCtt                                                                <  6:137773/149‑1 (MQ=255)
                                                                                    cGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTAACAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCtt                                                                <  2:214595/149‑1 (MQ=255)
                                                                                     gTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTtg                                                               >  1:136447/1‑149 (MQ=255)
                                                                                            cccAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATaa                                                         <  4:108912/148‑1 (MQ=255)
                                                                                                gCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGT‑GGCGCTTGTGATAACTATaa                                                   <  6:120217/149‑1 (MQ=255)
                                                                                                     gCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTgg                                                                      <  6:312573/126‑1 (MQ=255)
                                                                                                     gCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTgg                                                                      >  5:312573/1‑126 (MQ=255)
                                                                                                         tCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCgg                                            <  2:93609/148‑1 (MQ=255)
                                                                                                           aaaaCCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGGGCTTGTGATAACTATAACGCTCGGCAt                                         >  7:22148/1‑149 (MQ=255)
                                                                                                             aaCCCCTCATGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTTGGCGCTTGTGATAACTATAACGCTCGGCATTg                                       <  8:206479/149‑1 (MQ=255)
                                                                                                             aaCCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGTGCTTGTGATAACTATAACGCTCGGCATTg                                       <  8:22148/149‑1 (MQ=255)
                                                                                                                  cTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGc                                  <  5:219144/149‑1 (MQ=255)
                                                                                                                      gggCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCcgc                              >  3:308384/1‑149 (MQ=255)
                                                                                                                          gTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATg                          >  3:81641/1‑149 (MQ=255)
                                                                                                                               aTGAATGCAAAAAGCCCCGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTcc                     >  3:70562/1‑149 (MQ=255)
                                                                                                                                      atagaaGCCCAGCCCCGCGCTGGGCTTCTATCTGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGc              >  2:280926/5‑149 (MQ=38)
                                                                                                                                         aaaGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGAt                                                           >  8:215107/1‑101 (MQ=255)
                                                                                                                                         aaaGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGAt                                                           <  7:215107/101‑1 (MQ=255)
                                                                                                                                                 gcgcGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGATCTctgc   <  6:124459/149‑1 (MQ=255)
                                                                                                                                                  cgcgGGGCTGGGCTTCTATCATCTTGGCTGAACAGTAATTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGTTAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGATCTctgcc  <  4:308384/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ACCAGGTCGACTACACCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGATCTCTGCC  >  NC_002947/4301428‑4301722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑