Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 334,836 T→G 7.6% intergenic (‑151/+137) PP_0276 ← / ← PP_t73 Cro/CI family transcriptional regulator/tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947334,8360TG 7.6% 185.9 / 3.2 63intergenic (‑151/+137)PP_0276/PP_t73Cro/CI family transcriptional regulator/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base T (25/33);  new base G (3/2);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑AAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCAC  >  NC_002947/334688‑334984
                                                                                                                                                    |                                                                                                                                                     
gCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGATTTGGTTCTGGTTGTGGTGGTTGGGgctgct                                                                                                                                                       >  1:292237/1‑149 (MQ=255)
  ttGCCTCGGCAGAAATATCTAAAAATTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCtgctgc                                                                                                                                                     >  8:336558/1‑149 (MQ=255)
     ccTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCa                                                                                                                                                  <  2:325023/149‑1 (MQ=255)
     ccTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCa                                                                                                                                                  <  6:335991/149‑1 (MQ=255)
      cTCGGCAGAAATATCTAAAATTTGCGATAAATCGAAGTGTGGATTGGTGGGGGAGTCAAAGCGGTTGTGTGGGACTGTGGGTCTGTTTTTGCGCGGTGCTGGTGATGAGAGAATTCCTTCGGCGTGGGGTGGTTGGGGCTGCTgcgggg                                                                                                                                                 >  8:177858/1‑146 (MQ=255)
           cAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCAt                                                                                                                                            <  3:251096/149‑1 (MQ=255)
                atatCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAg                                                                                                                                       <  1:133634/149‑1 (MQ=255)
                     tAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAg                                                                                                                                  >  5:156558/1‑149 (MQ=255)
                     tAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAg                                                                                                                                  >  6:296414/1‑149 (MQ=255)
                     tAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAg                                                                                                                                  >  7:151620/1‑149 (MQ=255)
                     tAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAg                                                                                                                                  >  5:160993/1‑149 (MQ=255)
                       aaaTTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCaa                                                                                                                                   <  5:222871/146‑1 (MQ=255)
                                   aaTAGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATAAGAGAATTGCTTCTGCTTGGGTTGGTTGGGGCTGCTGATCAGCCCATCGCAGGAAAGCCATCTCCTACAcc                                                                                                                    >  7:278640/1‑149 (MQ=255)
                                    aTCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCGCAGCAGcccc                                                                                                                                             >  8:57565/1‑122 (MQ=255)
                                    aTCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCGCAGCAGcccc                                                                                                                                             <  7:57565/123‑2 (MQ=255)
                                       gTAGTGTGGATTGGTTGGCGAGTCGAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATc                                                                                                                <  5:344786/149‑1 (MQ=255)
                                             tgGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCgtggt                                                                                                           <  8:108575/148‑1 (MQ=255)
                                               gATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTCTCTGTTTTTGAGCTCTGCTAGTGATCAGAGAATTGCTTCTCCTTGGGGTGGTTGTGGCTGCTGCGAAGCCCATCGCAGGCAAGCCAGCTCATACACCGATCCtggtgg                                                                                                         >  1:62281/1‑148 (MQ=255)
                                                   ggttggCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGcc                                                                                                                                <  7:163128/121‑1 (MQ=255)
                                                   ggttggCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGcc                                                                                                                                >  8:163128/1‑121 (MQ=255)
                                                    gttggCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCa                                                                                                    <  3:305987/148‑1 (MQ=255)
                                                    gttggCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAg                                                                                                   <  7:336558/149‑1 (MQ=255)
                                                     ttggCGAGTCAAAGCGTTTGTGTTGGAGCGTGGGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGGGGTTGGGGCTGCGGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAgg                                                                                                  <  1:60725/149‑1 (MQ=255)
                                                     ttggCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAgg                                                                                                  <  2:89949/149‑1 (MQ=255)
                                                     ttggCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAgg                                                                                                  >  5:130005/1‑149 (MQ=255)
                                                     ttggCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTATTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAgg                                                                                                  <  5:180142/149‑1 (MQ=255)
                                                           aGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATcc                                                                                            >  1:250323/1‑149 (MQ=255)
                                                                aaGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTAGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAg                                                                                       >  5:240682/1‑149 (MQ=255)
                                                                    gTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTAGTTGGGGCTGCTGCGCAGCCCATCGCATGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAg                                                                                   <  6:240682/149‑1 (MQ=255)
                                                                         tgtTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGAt                                                                               <  8:151620/148‑1 (MQ=255)
                                                                           ttGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGAtt                                                                              >  1:329883/1‑147 (MQ=255)
                                                                                  gtgtgtCTTTTTTTGAGCTGTGGTGGTGATGATATAATTTCTTCTTCTTGGGGTGGTTGTGGCTGCTGCGCAGCCCATCGCAGGAAAGCCAGCTACTACAGCGATCGTGGTGGATTCAGGTCATCAGTAAGGCAGGGATTGATGGCTg                                                                      <  8:278640/148‑1 (MQ=255)
                                                                                     tgtCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCggg                                                                  <  2:250323/149‑1 (MQ=255)
                                                                                        cTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCgtggt                                                                                                           <  1:238742/105‑1 (MQ=255)
                                                                                        cTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCgtggt                                                                                                           <  1:243919/105‑1 (MQ=255)
                                                                                        cTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCgtggt                                                                                                           >  2:243919/1‑105 (MQ=255)
                                                                                        cTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCgtggt                                                                                                           >  2:238742/1‑105 (MQ=255)
                                                                                          gTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTaa                                                                                        >  7:318803/1‑122 (MQ=255)
                                                                                          gTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTaa                                                                                        <  8:318803/122‑1 (MQ=255)
                                                                                           tttttGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGaa                                                             <  5:296414/148‑1 (MQ=255)
                                                                                               tGAGCTGTGCTGGTGAGCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑a                                                        <  6:156558/148‑1 (MQ=255)
                                                                                                gAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑aa                                                       <  2:329883/148‑1 (MQ=255)
                                                                                                  gCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATAGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑aaaa                                                     >  7:192879/1‑148 (MQ=255)
                                                                                                   cTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑aaaaaa                                                   <  5:144096/149‑1 (MQ=255)
                                                                                                    tgtgCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGATGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑AAAAAAg                                                  >  2:222806/1‑149 (MQ=255)
                                                                                                      tgCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑AAAAAAGGc                                                >  7:300662/1‑149 (MQ=255)
                                                                                                      tgCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGGTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTTGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑AAAAAAGGc                                                <  8:240276/149‑1 (MQ=255)
                                                                                                       gCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCt                                                                       >  8:322781/1‑126 (MQ=255)
                                                                                                       gCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCt                                                                       <  7:322781/126‑1 (MQ=255)
                                                                                                        cTGGTGTTCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCACATCGGAGTGATGCGTGCTCGTACTCCGTTCGTGGTGGTATCAGGGGATCCGTAAGGGTGGGTTTGTGGGGTGGGGGGGGAAATC‑AAAAAAGGccc                                              <  2:62281/149‑1 (MQ=255)
                                                                                                        cTGGTGATCAGAGAATTTCTTCTGCTTGGGGGGGTTGGGTCTGCTGCGCAGCCCATCGCATGCAAGCCAGCTCCTACACCGATCGTGTTGTGTTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑AAAAAAGGcc                                               <  2:292237/148‑1 (MQ=255)
                                                                                                         tGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑AAAAAAGGCCCa                                             >  7:121154/1‑149 (MQ=255)
                                                                                                                cAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑AAAAAAGGCCCACCTTTCg                                      >  4:98224/1‑149 (MQ=255)
                                                                                                                     aaTTGGTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGTCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCTTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑AAAAAAGGCCCACCTTTCg                                      >  5:97158/1‑144 (MQ=255)
                                                                                                                      aTTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑AAAAAAGGCCCACCTTTCGGTGAGc                                <  3:98224/149‑1 (MQ=255)
                                                                                                                             cTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAAGGCCCACCTTTCGGTGAGCCtttt                           <  7:53929/148‑1 (MQ=255)
                                                                                                                               gCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑AAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGa                       >  7:86854/1‑149 (MQ=255)
                                                                                                                                cTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCt                                                                                                                            >  6:336812/1‑48 (MQ=255)
                                                                                                                                cTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCt                                                                                                                            <  5:336812/48‑1 (MQ=255)
                                                                                                                                cTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑AAAAAAGGCTCACCGAAAGGTGGGCCTTTTTTgt                       <  2:357332/148‑2 (MQ=39)
                                                                                                                                cTTGGCGTGGGTGGGGCCGCTGCGCAGCGCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTTAGGGAGGGATTGTGGGCGGCGGGGGGAAAAG‑AAAAAAGGCCCACCTTTCGGTGAGGCtttttt                         <  7:177858/146‑1 (MQ=255)
                                                                                                                                      ctgcgatgggCTGCGCAGCAGCCCCTCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑AAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCg                >  8:5215/8‑149 (MQ=255)
                                                                                                                                      ctgcgatgggCTGCGCAGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑AAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCg                >  6:208376/8‑149 (MQ=255)
                                                                                                                                       tggtTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑AAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGt               <  8:300662/149‑1 (MQ=255)
                                                                                                                                         gtTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTATGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑AAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTAt             >  5:350543/1‑149 (MQ=255)
                                                                                                                                                    tgcGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑AAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCAc  <  8:121154/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAGAGAATTGCTTCTGCTTGGGGTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAC‑AAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCGGCAC  >  NC_002947/334688‑334984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑