Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,344,302 A→T 10.9% intergenic (+33/‑123) PP_3817 → / → PP_3818 polyamine ABC transporter ATP‑binding protein/phosphate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,344,3020AT10.9% 120.2 / 8.7 56intergenic (+33/‑123)PP_3817/PP_3818polyamine ABC transporter ATP‑binding protein/phosphate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (29/18);  new base T (3/3);  total (33/23)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.19e-01

GGCATGCCGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCT  >  NC_002947/4344156‑4344450
                                                                                                                                                  |                                                                                                                                                    
ggCATGCCGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAg                                                                                                                                                     >  3:315401/1‑148 (MQ=255)
         aTGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCTGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgcccgc                                                                                                                                           <  2:103147/149‑1 (MQ=255)
               ccGGGCGACAGCGTCAAGGTGCGTTGGGCAGTGGCAGAAGATTGCATTTATACCGAATCGGCCCAGAGCGACCAGAGCAATTCGGCCGGGGCGATTTAACGCTGTTGTACGTTGCAGCGCCAGCCACATAGAGCACAGACCCCCCGGca                                                                                                                                     >  4:92739/1‑149 (MQ=255)
                     gACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGAt                                                                                                                               <  4:86023/149‑1 (MQ=255)
                          cGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGc                                                                                                                          <  7:242711/149‑1 (MQ=37)
                                 gTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCt                                                                                                                   >  2:234956/1‑149 (MQ=255)
                                    cGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCa                                                                                                                >  6:163115/1‑149 (MQ=255)
                                         ggCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACCCGATCTGGCAGGCGCTGCACTAtt                                                                                                           >  6:167362/1‑149 (MQ=255)
                                                  aGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCAGCCAGAACGACCACCGCCCGCGCGGCACACGATCAGGCACGCGCTGCACTAATGGAGGCGt                                                                                                   >  5:14870/1‑148 (MQ=255)
                                                    aCGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGc                                                                                                     <  3:24873/144‑1 (MQ=255)
                                                    aCGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGc                                                                                                     >  4:24873/1‑144 (MQ=255)
                                                        ttGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTgg                                                                                                         <  3:311552/136‑1 (MQ=255)
                                                        ttGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTgg                                                                                                         >  4:311552/1‑136 (MQ=255)
                                                            aTTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTca                                                                                        >  3:346512/1‑149 (MQ=255)
                                                              ttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTcaca                                                                                      >  1:227095/1‑149 (MQ=255)
                                                                   ccGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTat                                                                                  <  4:315401/148‑1 (MQ=255)
                                                                      aaTGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTatcatc                                                                              <  1:234956/149‑1 (MQ=255)
                                                                      aaTGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATAGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTatcat                                                                               >  1:143303/1‑148 (MQ=255)
                                                                                     aCCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTaa                                                               >  5:110263/1‑149 (MQ=255)
                                                                                      ccTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTgg                                                                                                                           >  1:189716/1‑88 (MQ=255)
                                                                                      ccTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTgg                                                                                                                           <  2:189716/88‑1 (MQ=255)
                                                                                       gtGAGTAAGTCGGCGGGGGGGCTTTAACGTGCGTTAAGGTTGCAGCGGGTGCCTTTTCGCGCCCCGCCGGCGGGGCACACGATGGGGGTGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCGTCTGTCGTAATc                                                             <  6:14870/148‑1 (MQ=255)
                                                                                        tGAGCAAGTCGGCCGGGGCGCATTAACGCGGGTGTTAGTTGCAGCGCCAGCCAGATAGCGCACCGCCCGCGCGGGCCACGCTCTGGCAGGGGCGGGCCTATTTGAGGCGGACGCCCCGGCCCATTTAGATAACGCCCCAGTCGTAAtat                                                            >  6:151131/1‑147 (MQ=255)
                                                                                            cAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCg                                                        <  5:163115/149‑1 (MQ=255)
                                                                                              aGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGc                                                      >  8:195642/1‑149 (MQ=255)
                                                                                              aGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGc                                                      >  8:111955/1‑149 (MQ=255)
                                                                                               gTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACGATTGGCGGCGTACGCCCCGGCACATTATCATCACGCCCCTGTCGTAATCTTCCGGc                                                      >  5:6401/1‑148 (MQ=255)
                                                                                                         gcgcATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGc                                           >  7:252403/1‑149 (MQ=255)
                                                                                                            cATTAACGCGCTTGTACGTTGCAGCGCCGGCCAGAGCGACCCCCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCcac                                        <  8:14873/149‑1 (MQ=255)
                                                                                                              ttAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAg                                      >  1:308722/1‑149 (MQ=255)
                                                                                                                 aCGCTCTTGTACGTTGCAGCGCCTGCCAGATCCAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGt                                    >  1:229933/1‑148 (MQ=255)
                                                                                                                   gCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCAc                                 >  5:3262/1‑149 (MQ=255)
                                                                                                                   gCTCTTGTACGTTGCAGCGCCTGCCAGATAGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCAAATTATCATCACGCCACTGTAGTAATATGCCGGCCACCTCTATGCCACAGGTCAc                                 >  2:216695/1‑149 (MQ=255)
                                                                                                                       ttGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTatc                                                                                 <  8:95676/97‑1 (MQ=255)
                                                                                                                       ttGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTatc                                                                                 >  7:95676/1‑97 (MQ=255)
                                                                                                                       ttGTACGTTGCAGCGCCCGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCgg                             >  4:3146/1‑149 (MQ=255)
                                                                                                                         gTACGTGCCAGCGCCGGCCAGATCGCGCACCCCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGc                            <  6:6401/148‑1 (MQ=255)
                                                                                                                             gTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCCTGCt                       >  1:320635/1‑149 (MQ=255)
                                                                                                                                gCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTcc                     <  4:346512/148‑1 (MQ=255)
                                                                                                                                     gcCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc               <  5:151131/149‑1 (MQ=255)
                                                                                                                                     gcCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc               <  6:3262/149‑1 (MQ=255)
                                                                                                                                       cTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGCGGCGTACGCCACGTCAAATTATCATCACGCCACTGTCGTTATCTGCCGGCAAACTCTATCGCACAGGTCACCCGGCAAGCTCCGCCTGCtt             >  8:134306/1‑149 (MQ=255)
                                                                                                                                        tGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCcaca                                       >  5:346298/1‑122 (MQ=25)
                                                                                                                                        tGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCcaca                                       >  3:367017/1‑122 (MQ=25)
                                                                                                                                        tGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCcaca                                       <  4:367017/122‑1 (MQ=25)
                                                                                                                                        tGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCcaca                                       <  6:346298/122‑1 (MQ=25)
                                                                                                                                          ccAGATCGTGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTc          >  2:261349/1‑149 (MQ=255)
                                                                                                                                           cAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCt         >  6:127188/1‑149 (MQ=255)
                                                                                                                                            aGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCtt             <  1:20297/144‑1 (MQ=255)
                                                                                                                                            aGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCtt             >  2:20297/1‑144 (MQ=255)
                                                                                                                                            aGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCtt        <  7:111955/149‑1 (MQ=255)
                                                                                                                                            aGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTCGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCtt        <  7:195642/149‑1 (MQ=255)
                                                                                                                                             gagcGAGCACCCCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtg       <  2:229933/146‑1 (MQ=255)
                                                                                                                                             gATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtg       >  7:121645/1‑149 (MQ=255)
                                                                                                                                             gATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtg       >  2:253254/1‑149 (MQ=255)
                                                                                                                                                 gAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctgc   <  8:252403/149‑1 (MQ=255)
                                                                                                                                                  aGCACCGCCCGCGCGGCACACGATCTGGAAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTgctgct  <  1:216695/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GGCATGCCGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCT  >  NC_002947/4344156‑4344450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑