Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,344,319 A→G 8.2% intergenic (+50/‑106) PP_3817 → / → PP_3818 polyamine ABC transporter ATP‑binding protein/phosphate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,344,3190AG 8.2% 164.2 / 4.9 62intergenic (+50/‑106)PP_3817/PP_3818polyamine ABC transporter ATP‑binding protein/phosphate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (31/25);  new base G (2/3);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

CCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTACCAC  >  NC_002947/4344171‑4344464
                                                                                                                                                    |                                                                                                                                                 
ccGGGCGACAGCGTCAAGGTGCGTTGGGCAGTGGCAGAAGATTGCATTTATACCGAATCGGCCCAGAGCGACCAGAGCAATTCGGCCGGGGCGATTTAACGCTGTTGTACGTTGCAGCGCCAGCCACATAGAGCACAGACCCCCCGGca                                                                                                                                                   >  4:92739/1‑149 (MQ=255)
      gACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGAt                                                                                                                                             <  4:86023/149‑1 (MQ=255)
           cGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGc                                                                                                                                        <  7:242711/149‑1 (MQ=37)
                  gTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCt                                                                                                                                 >  2:234956/1‑149 (MQ=255)
                     cGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCa                                                                                                                              >  6:163115/1‑149 (MQ=255)
                          ggCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACCCGATCTGGCAGGCGCTGCACTAtt                                                                                                                         >  6:167362/1‑149 (MQ=255)
                                   aGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCAGCCAGAACGACCACCGCCCGCGCGGCACACGATCAGGCACGCGCTGCACTAATGGAGGCGt                                                                                                                 >  5:14870/1‑148 (MQ=255)
                                     aCGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGc                                                                                                                   <  3:24873/144‑1 (MQ=255)
                                     aCGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGc                                                                                                                   >  4:24873/1‑144 (MQ=255)
                                         ttGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTgg                                                                                                                       >  4:311552/1‑136 (MQ=255)
                                         ttGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTgg                                                                                                                       <  3:311552/136‑1 (MQ=255)
                                             aTTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTca                                                                                                      >  3:346512/1‑149 (MQ=255)
                                               ttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTcaca                                                                                                    >  1:227095/1‑149 (MQ=255)
                                                    ccGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTat                                                                                                <  4:315401/148‑1 (MQ=255)
                                                       aaTGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTatcatc                                                                                            <  1:234956/149‑1 (MQ=255)
                                                       aaTGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATAGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTatcat                                                                                             >  1:143303/1‑148 (MQ=255)
                                                                      aCCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTaa                                                                             >  5:110263/1‑149 (MQ=255)
                                                                       ccTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTgg                                                                                                                                         >  1:189716/1‑88 (MQ=255)
                                                                       ccTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTgg                                                                                                                                         <  2:189716/88‑1 (MQ=255)
                                                                        gtGAGTAAGTCGGCGGGGGGGCTTTAACGTGCGTTAAGGTTGCAGCGGGTGCCTTTTCGCGCCCCGCCGGCGGGGCACACGATGGGGGTGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCGTCTGTCGTAATc                                                                           <  6:14870/148‑1 (MQ=255)
                                                                         tGAGCAAGTCGGCCGGGGCGCATTAACGCGGGTGTTAGTTGCAGCGCCAGCCAGATAGCGCACCGCCCGCGCGGGCCACGCTCTGGCAGGGGCGGGCCTATTTGAGGCGGACGCCCCGGCCCATTTAGATAACGCCCCAGTCGTAAtat                                                                          >  6:151131/1‑147 (MQ=255)
                                                                             cAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCg                                                                      <  5:163115/149‑1 (MQ=255)
                                                                               aGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGc                                                                    >  8:195642/1‑149 (MQ=255)
                                                                               aGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGc                                                                    >  8:111955/1‑149 (MQ=255)
                                                                                gTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACGATTGGCGGCGTACGCCCCGGCACATTATCATCACGCCCCTGTCGTAATCTTCCGGc                                                                    >  5:6401/1‑148 (MQ=255)
                                                                                          gcgcATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGc                                                         >  7:252403/1‑149 (MQ=255)
                                                                                             cATTAACGCGCTTGTACGTTGCAGCGCCGGCCAGAGCGACCCCCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCcac                                                      <  8:14873/149‑1 (MQ=255)
                                                                                               ttAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAg                                                    >  1:308722/1‑149 (MQ=255)
                                                                                                  aCGCTCTTGTACGTTGCAGCGCCTGCCAGATCCAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGt                                                  >  1:229933/1‑148 (MQ=255)
                                                                                                    gCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCAc                                               >  5:3262/1‑149 (MQ=255)
                                                                                                    gCTCTTGTACGTTGCAGCGCCTGCCAGATAGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCAAATTATCATCACGCCACTGTAGTAATATGCCGGCCACCTCTATGCCACAGGTCAc                                               >  2:216695/1‑149 (MQ=255)
                                                                                                        ttGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTatc                                                                                               >  7:95676/1‑97 (MQ=255)
                                                                                                        ttGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTatc                                                                                               <  8:95676/97‑1 (MQ=255)
                                                                                                        ttGTACGTTGCAGCGCCCGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCgg                                           >  4:3146/1‑149 (MQ=255)
                                                                                                          gTACGTGCCAGCGCCGGCCAGATCGCGCACCCCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGc                                          <  6:6401/148‑1 (MQ=255)
                                                                                                              gTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCCTGCt                                     >  1:320635/1‑149 (MQ=255)
                                                                                                                 gCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTcc                                   <  4:346512/148‑1 (MQ=255)
                                                                                                                      gcCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc                             <  5:151131/149‑1 (MQ=255)
                                                                                                                      gcCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc                             <  6:3262/149‑1 (MQ=255)
                                                                                                                        cTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGCGGCGTACGCCACGTCAAATTATCATCACGCCACTGTCGTTATCTGCCGGCAAACTCTATCGCACAGGTCACCCGGCAAGCTCCGCCTGCtt                           >  8:134306/1‑149 (MQ=255)
                                                                                                                         tGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCcaca                                                     <  4:367017/122‑1 (MQ=25)
                                                                                                                         tGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCcaca                                                     >  5:346298/1‑122 (MQ=25)
                                                                                                                         tGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCcaca                                                     >  3:367017/1‑122 (MQ=25)
                                                                                                                         tGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCcaca                                                     <  6:346298/122‑1 (MQ=25)
                                                                                                                           ccAGATCGTGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTc                        >  2:261349/1‑149 (MQ=255)
                                                                                                                            cAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCt                       >  6:127188/1‑149 (MQ=255)
                                                                                                                             aGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCtt                           >  2:20297/1‑144 (MQ=255)
                                                                                                                             aGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCtt                           <  1:20297/144‑1 (MQ=255)
                                                                                                                             aGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCtt                      <  7:111955/149‑1 (MQ=255)
                                                                                                                             aGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTCGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCtt                      <  7:195642/149‑1 (MQ=255)
                                                                                                                              gagcGAGCACCCCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtg                     <  2:229933/146‑1 (MQ=255)
                                                                                                                              gATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtg                     >  7:121645/1‑149 (MQ=255)
                                                                                                                              gATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtg                     >  2:253254/1‑149 (MQ=255)
                                                                                                                                  gAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctgc                 <  8:252403/149‑1 (MQ=255)
                                                                                                                                   aGCACCGCCCGCGCGGCACACGATCTGGAAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTgctgct                <  1:216695/149‑1 (MQ=255)
                                                                                                                                           ccgcGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGc        <  1:261349/149‑1 (MQ=255)
                                                                                                                                             gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTa      >  3:359698/1‑149 (MQ=255)
                                                                                                                                              cgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTAc     <  6:110263/149‑1 (MQ=255)
                                                                                                                                               gcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTc                                    <  7:194319/117‑1 (MQ=255)
                                                                                                                                               gcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTc                                    >  8:194319/1‑117 (MQ=255)
                                                                                                                                               gcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgc                    <  4:200852/133‑1 (MQ=255)
                                                                                                                                               gcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgc                    >  3:200852/1‑133 (MQ=255)
                                                                                                                                                 ggCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTACCAc  <  3:3146/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTACCAC  >  NC_002947/4344171‑4344464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑