Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,344,321 A→T 8.0% intergenic (+52/‑104) PP_3817 → / → PP_3818 polyamine ABC transporter ATP‑binding protein/phosphate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,344,3210AT 8.0% 162.9 / 4.4 62intergenic (+52/‑104)PP_3817/PP_3818polyamine ABC transporter ATP‑binding protein/phosphate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (31/25);  new base T (2/3);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.82e-01

GACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTACCACTCGC  >  NC_002947/4344177‑4344468
                                                                                                                                                |                                                                                                                                                   
gACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGAt                                                                                                                                                 <  4:86023/149‑1 (MQ=255)
     cGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGc                                                                                                                                            <  7:242711/149‑1 (MQ=37)
            gTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCt                                                                                                                                     >  2:234956/1‑149 (MQ=255)
               cGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCa                                                                                                                                  >  6:163115/1‑149 (MQ=255)
                    ggCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACCCGATCTGGCAGGCGCTGCACTAtt                                                                                                                             >  6:167362/1‑149 (MQ=255)
                             aGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCAGCCAGAACGACCACCGCCCGCGCGGCACACGATCAGGCACGCGCTGCACTAATGGAGGCGt                                                                                                                     >  5:14870/1‑148 (MQ=255)
                               aCGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGc                                                                                                                       >  4:24873/1‑144 (MQ=255)
                               aCGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGc                                                                                                                       <  3:24873/144‑1 (MQ=255)
                                   ttGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTgg                                                                                                                           >  4:311552/1‑136 (MQ=255)
                                   ttGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTgg                                                                                                                           <  3:311552/136‑1 (MQ=255)
                                       aTTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTca                                                                                                          >  3:346512/1‑149 (MQ=255)
                                         ttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTcaca                                                                                                        >  1:227095/1‑149 (MQ=255)
                                              ccGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTat                                                                                                    <  4:315401/148‑1 (MQ=255)
                                                 aaTGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTatcatc                                                                                                <  1:234956/149‑1 (MQ=255)
                                                 aaTGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATAGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTatcat                                                                                                 >  1:143303/1‑148 (MQ=255)
                                                                aCCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTaa                                                                                 >  5:110263/1‑149 (MQ=255)
                                                                 ccTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTgg                                                                                                                                             >  1:189716/1‑88 (MQ=255)
                                                                 ccTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTgg                                                                                                                                             <  2:189716/88‑1 (MQ=255)
                                                                  gtGAGTAAGTCGGCGGGGGGGCTTTAACGTGCGTTAAGGTTGCAGCGGGTGCCTTTTCGCGCCCCGCCGGCGGGGCACACGATGGGGGTGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCGTCTGTCGTAATc                                                                               <  6:14870/148‑1 (MQ=255)
                                                                   tGAGCAAGTCGGCCGGGGCGCATTAACGCGGGTGTTAGTTGCAGCGCCAGCCAGATAGCGCACCGCCCGCGCGGGCCACGCTCTGGCAGGGGCGGGCCTATTTGAGGCGGACGCCCCGGCCCATTTAGATAACGCCCCAGTCGTAAtat                                                                              >  6:151131/1‑147 (MQ=255)
                                                                       cAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCg                                                                          <  5:163115/149‑1 (MQ=255)
                                                                         aGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGc                                                                        >  8:195642/1‑149 (MQ=255)
                                                                         aGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGc                                                                        >  8:111955/1‑149 (MQ=255)
                                                                          gTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACGATTGGCGGCGTACGCCCCGGCACATTATCATCACGCCCCTGTCGTAATCTTCCGGc                                                                        >  5:6401/1‑148 (MQ=255)
                                                                                    gcgcATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGc                                                             >  7:252403/1‑149 (MQ=255)
                                                                                       cATTAACGCGCTTGTACGTTGCAGCGCCGGCCAGAGCGACCCCCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCcac                                                          <  8:14873/149‑1 (MQ=255)
                                                                                         ttAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAg                                                        >  1:308722/1‑149 (MQ=255)
                                                                                            aCGCTCTTGTACGTTGCAGCGCCTGCCAGATCCAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGt                                                      >  1:229933/1‑148 (MQ=255)
                                                                                              gCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCAc                                                   >  5:3262/1‑149 (MQ=255)
                                                                                              gCTCTTGTACGTTGCAGCGCCTGCCAGATAGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCAAATTATCATCACGCCACTGTAGTAATATGCCGGCCACCTCTATGCCACAGGTCAc                                                   >  2:216695/1‑149 (MQ=255)
                                                                                                  ttGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTatc                                                                                                   >  7:95676/1‑97 (MQ=255)
                                                                                                  ttGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTatc                                                                                                   <  8:95676/97‑1 (MQ=255)
                                                                                                  ttGTACGTTGCAGCGCCCGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCgg                                               >  4:3146/1‑149 (MQ=255)
                                                                                                    gTACGTGCCAGCGCCGGCCAGATCGCGCACCCCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGc                                              <  6:6401/148‑1 (MQ=255)
                                                                                                        gTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCCTGCt                                         >  1:320635/1‑149 (MQ=255)
                                                                                                           gCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTcc                                       <  4:346512/148‑1 (MQ=255)
                                                                                                                gcCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc                                 <  5:151131/149‑1 (MQ=255)
                                                                                                                gcCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc                                 <  6:3262/149‑1 (MQ=255)
                                                                                                                  cTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGCGGCGTACGCCACGTCAAATTATCATCACGCCACTGTCGTTATCTGCCGGCAAACTCTATCGCACAGGTCACCCGGCAAGCTCCGCCTGCtt                               >  8:134306/1‑149 (MQ=255)
                                                                                                                   tGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCcaca                                                         <  6:346298/122‑1 (MQ=25)
                                                                                                                   tGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCcaca                                                         <  4:367017/122‑1 (MQ=25)
                                                                                                                   tGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCcaca                                                         >  3:367017/1‑122 (MQ=25)
                                                                                                                   tGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCcaca                                                         >  5:346298/1‑122 (MQ=25)
                                                                                                                     ccAGATCGTGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTc                            >  2:261349/1‑149 (MQ=255)
                                                                                                                      cAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCt                           >  6:127188/1‑149 (MQ=255)
                                                                                                                       aGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCtt                               <  1:20297/144‑1 (MQ=255)
                                                                                                                       aGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCtt                               >  2:20297/1‑144 (MQ=255)
                                                                                                                       aGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCtt                          <  7:111955/149‑1 (MQ=255)
                                                                                                                       aGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTCGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCtt                          <  7:195642/149‑1 (MQ=255)
                                                                                                                        gagcGAGCACCCCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtg                         <  2:229933/146‑1 (MQ=255)
                                                                                                                        gATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtg                         >  7:121645/1‑149 (MQ=255)
                                                                                                                        gATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtg                         >  2:253254/1‑149 (MQ=255)
                                                                                                                            gAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctgc                     <  8:252403/149‑1 (MQ=255)
                                                                                                                             aGCACCGCCCGCGCGGCACACGATCTGGAAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTgctgct                    <  1:216695/149‑1 (MQ=255)
                                                                                                                                     ccgcGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGc            <  1:261349/149‑1 (MQ=255)
                                                                                                                                       gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTa          >  3:359698/1‑149 (MQ=255)
                                                                                                                                        cgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTAc         <  6:110263/149‑1 (MQ=255)
                                                                                                                                         gcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTc                                        <  7:194319/117‑1 (MQ=255)
                                                                                                                                         gcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTc                                        >  8:194319/1‑117 (MQ=255)
                                                                                                                                         gcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgc                        >  3:200852/1‑133 (MQ=255)
                                                                                                                                         gcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgc                        <  4:200852/133‑1 (MQ=255)
                                                                                                                                           ggCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTACCAc      <  3:3146/149‑1 (MQ=255)
                                                                                                                                               cacGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTACCACTcgc  >  4:20142/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTACCACTCGC  >  NC_002947/4344177‑4344468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑