| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 90.5% | 61.4 / 1.0 | 21 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (8/11); total (9/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
TCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑G‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCAC > NC_002947/1932499‑1932775 | tCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATCCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGaa < 7:2099/148‑1 (MQ=255) cTTCGAAGAGGATCTGGACATTCGACAGCGGCATGGCAGGGCTCACGAAAATAGCAGTAACCGCTGGGGCCGCTTTGCGGCCCGTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAg < 5:45446/148‑1 (MQ=38) ttCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGCGC‑GGGCTT‑GCCCGCGAAgg > 3:15012/1‑148 (MQ=255) gATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTACCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAgg > 2:143061/1‑148 (MQ=255) aCATTCCACAGCGCCATTGCAGGTCTCACTAAAATCGCAGTAAACGGTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGTATATGCGCCCCCTGTCGGCGG‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGc < 2:123938/145‑1 (MQ=37) cGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCCCGCCGACAAAGGATATGCGTCCCCAGTAGGGGCGGGGGTG‑G‑CCGCGCAGGGCAGCAAGGCAGCCCCaaaat > 6:11079/1‑146 (MQ=255) gCATTGCAGGTCTCACTAAAATAGCAGTAAACGCGGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGTATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTa < 4:50138/148‑1 (MQ=37) gCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCATACAAAGGATATGCGTCCCCGGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAATGCAGCCCCAAATTGTCGACTa > 8:15640/1‑148 (MQ=38) aaaaTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCAACAACAAAGGCTATGCGTCCCCTGTAGGAGC‑GGGGTT‑GCCCGCGACGGGCTGCAAGGAAGCCACAAAATGTAGACTACGCGGCTCCGCGCTCCg > 8:39958/1‑148 (MQ=25) aGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAgg < 8:128504/148‑1 (MQ=255) ccGCTGGGGCCGCTTGGCGGCCCGTCGCGGGCACCCCCGCGCCACCAAAGGATATGCGCCCCCTGTAGGCGC‑GGGCTTCC‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGtt < 5:10330/147‑1 (MQ=255) ggCCCTTCGCGGGCACGCCCGCTCCTACAAAGTATATGCGGCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagc < 5:133566/147‑1 (MQ=39) ggCAAGCCCGCTCCTACAACGGATAGGCGGCCCCTGTAGGTGC‑GGGCTG‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGt < 3:36714/148‑1 (MQ=255) ccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAg > 2:36380/1‑148 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGc > 4:149277/1‑148 (MQ=255) gATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcat < 6:5004/148‑1 (MQ=255) gCGTCCCGTGTAGGAGG‑GGGCTG‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGTAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc < 2:41491/147‑1 (MQ=255) gTCCCCTGTAGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGtt > 3:161162/1‑148 (MQ=255) aGGAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATg < 1:143061/146‑1 (MQ=255) gAGC‑GGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca < 7:14177/148‑1 (MQ=255) gC‑GGGCTT‑GCCCGTGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGCGCAGCTGCCCTGGTTTTCCAGCTAGATATGCACATCATCGTTGCCGATGTTcac > 8:95847/1‑147 (MQ=255) | TCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑G‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCAC > NC_002947/1932499‑1932775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |