| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 3,845,705 | +G | intergenic (‑72/+26) | PP_3394 ← / ← PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,845,703 | 1 | . | G | 92.0% | 75.9 / 0.8 | 25 | intergenic (‑70/+28) | PP_3394/PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (14/9); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01 | |||||||||||
ATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAA > NC_002947/3845569‑3845840 | aTGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATc < 6:183981/148‑1 (MQ=255) tctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGAt < 6:143772/148‑1 (MQ=255) tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGAAGTGTTTC‑GCGGGCTTATCCATCGTCAGCCACGATGGATCa > 5:112185/1‑148 (MQ=255) gCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAg > 3:68891/1‑148 (MQ=255) cAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGc > 6:131941/1‑148 (MQ=255) aaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAgtc > 5:30427/1‑146 (MQ=255) gCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGGAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCcg < 8:165885/148‑1 (MQ=255) cGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGa > 7:6439/1‑126 (MQ=255) gACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGAt < 8:6439/126‑1 (MQ=255) gtttgtttttgttGTCCTCGCGGGCTGTGTGTGTGACGCGTGGCGCCAGACTATGAGGTGGAGGCCGGGCAGAAAAGCGGTTTTGCGGG‑GGCGGACCCATCGTCCGCCACGTGGCATCTGCCCGCGCCCTGCCAGGCCGGTGCACgc < 2:14849/145‑1 (MQ=255) tattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCCGCCCCGATGGATCAGCCCGCGCCCGGCCAGGCCGGTGCACGCAGCcaca > 1:164846/1‑148 (MQ=255) gttgtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCCCGCCCTGCCACGCCCGTGCCCGCAGCAACAggtg > 2:81450/1‑148 (MQ=255) tgccATCGCGGGCTCTCTGTCTCACGCTGGGCGCCAGCCTACCCGCGGGATCCCGGGCTCAAAACACGTGTTGC‑GCGGGCTCCCCCATCGGCAGCCCCCCGGGATCCGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtgg < 8:60411/144‑1 (MQ=255) tgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGCTGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACa > 5:91255/1‑148 (MQ=255) aaGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAg > 7:126030/1‑147 (MQ=255) gggTTGCGCCATACTAAGAGGTGGATGCCGGGCATAAAAGAGGTGTTTCGGG‑GGGTGATCCATCGTCAGCCACTTTGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCga < 6:91255/146‑1 (MQ=255) gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCgtg > 2:40687/1‑148 (MQ=255) cccAGAATAAGCGCTGCATCACGGGCAGAATCGCCTGGTGCC‑GCGGGCGGAGCCAGCGGCCGCCAGGAGGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCgtg < 5:131941/147‑1 (MQ=255) ccAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGCGCCGACAGCGAGAGCgtgt > 3:49115/1‑148 (MQ=255) ccGGGCAGAAACCCCGGGTTGC‑GCGGGCGGACCCATCGCCCCCCACGATGGATAAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGAt < 7:172436/147‑1 (MQ=255) cGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATg > 8:173012/1‑148 (MQ=255) ggCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGCTGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCAACATCGAGAGCGTGTGCCGGTCGCGCATGCAGCTGa > 6:151588/1‑147 (MQ=255) aaaaGACGTGTTTC‑GCGGGCTGATCCATCGGCAGCCACAATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAAtt < 1:29721/148‑1 (MQ=255) aaaGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTc > 4:166986/1‑148 (MQ=255) ggtgtTTC‑GCGGGCTGATCCATCGTCAGCCACGATGTATCTGCCCGCGCCGTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCaa < 8:126030/147‑1 (MQ=255) | ATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAA > NC_002947/3845569‑3845840 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |