Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,580,995	C→A	K134N (AAG→AAT)	ttgB ←	efflux pump membrane protein TtgB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,580,995	0	C	A	100.0%	91.9 / NA	28	K134N (AAG→AAT)	ttgB	efflux pump membrane protein TtgB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/16);  total (12/16)

GTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTCA  >  NC_002947/1580851‑1581134                                                                                                                                                 |                                                                                                                                            gTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGttattc                                                                                                                                          >  8:120672/1‑148 (MQ=255)        gcACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCtt                                                                                                                                   >  8:17046/1‑148 (MQ=255)             gAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTg                                                                                                                              <  1:180486/148‑1 (MQ=255)              aaCACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGa                                                                                                                             >  3:152396/1‑148 (MQ=255)                 acCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGAcgc                                                                                                                          <  4:178043/148‑1 (MQ=255)                    tGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGa                                                                                                                       <  4:84725/148‑1 (MQ=255)                    tGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGGCCTGCATGTTCGAGACGATTTAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGa                                                                                                                       <  1:186573/148‑1 (MQ=255)                                  acCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCAATGCTTTGGTGACGAGGATACCTtgctgctgc                                                                                                         >  6:10348/1‑148 (MQ=255)                                      gcgGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACtt                                                                                                     <  5:132901/148‑1 (MQ=255)                                         gTACGCGAGAGCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCt                                                                                                  <  1:37305/148‑1 (MQ=255)                                             gcgAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGCCCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCg                                                                                               >  5:8584/1‑147 (MQ=255)                                               gagaTCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGgca                                                                                            >  8:74004/1‑148 (MQ=255)                                                     gggTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCg                                                                                      >  2:138863/1‑148 (MQ=255)                                                       gTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCggg                                                                                    <  6:49501/148‑1 (MQ=255)                                                           tGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCCGCAGCGGGGTgg                                                                                >  4:103301/1‑148 (MQ=255)                                                           tGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACAAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTgg                                                                                >  4:24931/1‑148 (MQ=255)                                                             cATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTgt                                                                                >  5:60170/1‑145 (MQ=255)                                                                      gaCGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGc                                                                     <  2:14268/148‑1 (MQ=255)                                                                            ataGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTc                                                               <  3:160378/148‑1 (MQ=255)                                                                              aGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTg                                                                  >  5:95499/1‑143 (MQ=255)                                                                               gTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGt                                                                 <  6:95499/143‑1 (MQ=255)                                                                                ttGGCCAGGTCGTCCTTGGTCATTCTGCCGTCTTCTGAGACCTGGCCGATCACCAGCTGGAAGTTATTCACTGCTTTTGTGACGCTGATACCTTGCTGCTGCACTTCCTGCGGCAGCTGCGGGGTGGCCAGGTTCAGCTTGTTctgta                                                           <  6:63100/148‑1 (MQ=255)                                                                                              cTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGcgcg                                             <  7:120672/148‑1 (MQ=255)                                                                                                       gctgcCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCgggg                                    <  6:124092/148‑1 (MQ=255)                                                                                                            cGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGt                               <  7:17046/148‑1 (MQ=255)                                                                                                                ttCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGccc                           >  1:42239/1‑148 (MQ=255)                                                                                                                               gATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCg            <  4:57422/148‑1 (MQ=255)                                                                                                                                     cagcagGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATg      >  5:93177/1‑148 (MQ=255)                                                                                                                                         agGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTCa  <  4:152396/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                            GTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTCA  >  NC_002947/1580851‑1581134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑