Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	3,232,944	G→A	M44I (ATG→ATA)	GbpR →	galactose‑binding protein regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,232,944	0	G	A	100.0%	91.3 / NA	28	M44I (ATG→ATA)	GbpR	galactose‑binding protein regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/14);  total (14/14)

TACCGGATTCGATATGTCTCGCTTGCCACCTGACAAGGCTCAGGCGCCTGCCAGCCTCGGTGCCTTGAAGATTTCCAGCCGTCAGATCACGTTGCTCAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATGCACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTACGTCTGTTCGAGCGCCTGCCCCGGGGGATGCAAGCGACGCTGTATGGCGAGGTGATGATTCGCTATGCGCAAAACGCATTGCA  >  NC_002947/3232800‑3233087                                                                                                                                                 |                                                                                                                                                tACCGGATTCGATATGTCTCGCTTGCCACCTGACAAGGCTCAGGCGCCTGCCAGCCTCGGTGCCTTGAAGATTTCCAGCCGTCAGATCACGTTGCTCAATGCTCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATacac                                                                                                                                              >  6:126237/1‑148 (MQ=255)           gATATGTCTCGCTTGCCACCTGACAAGGCTCAGGCGCCTGCCAGCCTCGGTGCCTTGAAGATTTCCAGCCGTCAGATCACGTTGCTCAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAAc                                                                                                                                    >  7:166498/1‑148 (MQ=255)                 tctcGCTTGCCACCTGACAAGGCTCAGGCGCCTGCCAGCCTCGGTGCCTTGAAGATTTCCAGCCGTCAGATCACGTTGCTCAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGgccg                                                                                                                              >  4:74534/1‑148 (MQ=255)                       ttGCCACCTGACAAGGCTCAGGCGCCTGCCAGCCTCGGTGCCTTGAAGATTTCCAGCCGTCAGATCACGTTGCTCAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGGCCGCCAGcc                                                                                                                        >  5:47379/1‑148 (MQ=255)                            aCCTGACAAGGCTCGGGCGCCTGCCAGCCTCGGTGCCTTGAAGATTTCCAGCCGTCAGATCACGTTGCTCAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCACCCGGCCGCCAGCCTGTTg                                                                                                                   >  1:69538/1‑148 (MQ=255)                              cTGACAAGGCTCAGGCGCCTGCCAGCCTCGGTGCCTTGAAGATTTCCAGCCGTCAGATGACGTTGCTCAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCGGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCt                                                                                                                 <  6:134715/148‑1 (MQ=255)                                          aGGCGCCTGCCAGCCTCGGTGCCTTGAAGATTTCCAGCCGTCAGATCACGTTGCTCAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTga                                                                                                     >  6:178842/1‑148 (MQ=255)                                           ggCGCCTGCCAGCCTCGGTGCCTTGAAGATTTCCAGCCGTCAGATCACGTTGCTCAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTgag                                                                                                    <  4:114784/148‑1 (MQ=255)                                                     aGCCTCGGTGCCTTGAAGATTTCCAGCCGTCAGATCACGTTGCTCAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTgg                                                                                          <  3:12783/148‑1 (MQ=255)                                                        cTCGGTGCCTTGAAGATTTCCAGCCGTCAGATCACGTTGCTCAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTgggg                                                                                        >  6:6276/1‑147 (MQ=255)                                                               ccTTGAAGATTTCCAGCCGTCAGATCACGTTGCTCAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTACGTCt                                                                                >  6:52920/1‑148 (MQ=255)                                                                 ttGAAGATTTCCAGCCGTCAGATCACGTTGCTCAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTACGTCTGt                                                                              >  5:33445/1‑148 (MQ=255)                                                                     aGATTTCCAGCCGTCAGATCACGTTGCTCAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTACGTCTGTTCGa                                                                          <  3:56705/148‑1 (MQ=255)                                                                                   cAGATCACGTTGCTCAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTACGTCTGTTCGAGCGCCTGCCCCggg                                                            <  8:166498/148‑1 (MQ=255)                                                                                     gATCACGTTGCTCAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACAACGCAACCTGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTACGTCTGTTCGAGCGCCTGCCCCggggg                                                          >  1:31909/1‑148 (MQ=255)                                                                                           gTTGCTCAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTACGTCTGTTCGAGCGCCTGCCCCGGGGGATGCaa                                                    >  2:95733/1‑148 (MQ=255)                                                                                                tCAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTa                                                                                     >  3:56864/1‑110 (MQ=255)                                                                                                 cAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTAc                                                                                    <  4:56864/110‑1 (MQ=255)                                                                                                   aTGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTACGTCTGTTCGAGCGCCTGCCCCGGGGGATGCAAGCGACGCt                                            <  5:178842/148‑1 (MQ=255)                                                                                                    tGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTACGTCTGTTCGAGCGCCTGCCCCGGGGGATGCAAGCGACGCTg                                           <  5:52920/148‑1 (MQ=255)                                                                                                          gggCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTACGTCTGTTCGAGCGCCTGCCCCGGGGGATGCAAGCGACGCTGTATgg                                      >  1:144134/1‑147 (MQ=255)                                                                                                              gAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGAAGCTTGAGTATCGCATGGGGGTACGTCTGTTCGAGCGCCTGCCCCGGGGGATGCAAGCGACGCTGTATGGCGAgg                                 <  5:126151/148‑1 (MQ=255)                                                                                                                    ggCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTACGTCTGTTCGAGCTCCTGCCCCGGGGGATGCAAGCGACGCTGTATGGCGAGGtgatg                            <  6:33445/147‑1 (MQ=255)                                                                                                                    ggCAACCTGCGCCGCGCCGCCGCCGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTACGTCTGTTCGAGCGCCTGCCCCGGGGGATGCAAGCGACGCTGTATGGCGAGGtgatg                            <  5:126237/147‑1 (MQ=255)                                                                                                                              gccgcgccgcCGCCGCGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTACGTCTGTTCGAGCGCCTGCCCCGGGGGATGCAAGCGACGCTGTATGGCGAGGTGATGATTCGCTATgc                 >  3:117726/1‑148 (MQ=255)                                                                                                                                     cgccgccgcGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTACGTCTGTTCGAGCGCCTGCCCCGGGGGATGCAAGCGACGCTGTATGGCGAGGTGATGATTCGCTATGCGCAAAAc          <  6:47379/148‑1 (MQ=255)                                                                                                                                        cgccgcGATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTACGTCTGTTCGAGCGCCTGCCCCGGGGGATGCAAGCGACGCTGTATGGCGAGGTGATGATTCGCTATGCGCAAAACGCa       <  5:6276/148‑1 (MQ=255)                                                                                                                                             cgATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTACGTCTGTTCGAGCGCCTGCCCCGGGGGATGCAAGCGACGCTGTATGGCGAGGTGATGATTCGCTATGCGCAAAACGCATTGCa  <  2:18113/148‑1 (MQ=255)                                                                                                                                              gATACACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTACGTCTGTTCGAGCGCCTGCCCCGGGGGATGCAAGCGACGCTGTATGGCGAGGTGATGATTCGCTATGCGCAAAACGCATTGCa  >  1:4615/1‑147 (MQ=255)                                                                                                                                                 |                                                                                                                                                TACCGGATTCGATATGTCTCGCTTGCCACCTGACAAGGCTCAGGCGCCTGCCAGCCTCGGTGCCTTGAAGATTTCCAGCCGTCAGATCACGTTGCTCAATGCGCTGGGCGAATTCGGCAACCTGCGCCGCGCCGCCGCCGCGATGCACACCACGCAACCGGCCGCCAGCCTGTTGCTACAGCAGCTTGAGGAGCGCCTGGGGGTACGTCTGTTCGAGCGCCTGCCCCGGGGGATGCAAGCGACGCTGTATGGCGAGGTGATGATTCGCTATGCGCAAAACGCATTGCA  >  NC_002947/3232800‑3233087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑