Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,733,446	T→C	Y730C (TAT→TGT)	sucA ←	2‑oxoglutarate dehydrogenase subunit E1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,733,446	0	T	C	100.0%	126.0 / NA	37	Y730C (TAT→TGT)	sucA	2‑oxoglutarate dehydrogenase subunit E1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/20);  total (17/20)

GCAGCGGGCGAATGACCTGGCGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCATAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTGGGCTTCCCAGATCACCAGCGCGTTCGGCATG  >  NC_002947/4733303‑4733589                                                                                                                                                 |                                                                                                                                                gcAGCGGGCGAATGACCTGGCGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCCGTACGCAGACCTCGATGTTGTGCTCGGCGCACAGCTGAAGGTAACGATCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGACCTTCACAGcc                                                                                                                                             >  8:48849/1‑148 (MQ=255)   aGCGGGCGAATGACCTGGCGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCgt                                                                                                                                           <  3:33012/148‑1 (MQ=255)        gCGAATGACCTGGCGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGgc                                                                                                                                      <  2:161979/148‑1 (MQ=255)                ccTGGCGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCAt                                                                                                                              >  7:97759/1‑148 (MQ=255)                     cGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTc                                                                                                                          <  8:19932/147‑1 (MQ=255)                     cGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTc                                                                                                                          <  1:29621/147‑1 (MQ=255)                       aCGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTAGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGa                                                                                                                       <  8:43797/148‑1 (MQ=255)                            gcagGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGATCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGc                                                                                                                   >  6:116630/1‑147 (MQ=255)                              agGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGcaca                                                                                                                >  3:163392/1‑148 (MQ=255)                                gtggtAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTgg                                                                                                                                       >  1:123306/1‑123 (MQ=255)                                 tggtAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGgc                                                                                                                                      <  2:123306/123‑1 (MQ=255)                                  ggtAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTTGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAggc                                                                                                             >  6:100019/1‑148 (MQ=255)                                       aTCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGcccc                                                                                                       <  2:171528/148‑1 (MQ=255)                                             gCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCAc                                                                                                     <  5:23620/144‑1 (MQ=255)                                                 gCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTc                                                                                             >  8:132995/1‑148 (MQ=255)                                                                    cTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTgg                                                                          >  6:38590/1‑148 (MQ=255)                                                                     tGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGt                                                                         <  5:38590/148‑1 (MQ=255)                                                                     ctgcagctgtgCGCCGAGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGt                                                                         >  2:181113/8‑148 (MQ=255)                                                                         agctgtgCGCCGAGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGa                                                                      >  1:100009/4‑147 (MQ=255)                                                                                 tCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACtt                                                             >  6:43204/1‑148 (MQ=255)                                                                                          aGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGt                                                    <  5:43204/148‑1 (MQ=255)                                                                                            cTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTg                                                  <  1:181113/148‑1 (MQ=255)                                                                                            cTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCGCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTg                                                  <  2:165882/148‑1 (MQ=255)                                                                                            atGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACAGCACAGGCTGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTg                                                  <  7:53466/147‑1 (MQ=255)                                                                                             tGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTgg                                                 <  3:162273/148‑1 (MQ=255)                                                                                                     aCGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCg                                         >  7:127959/1‑148 (MQ=255)                                                                                                         tCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGa                                     <  5:116630/148‑1 (MQ=255)                                                                                                             gACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAggcg                                                                                                            >  2:120608/1‑73 (MQ=255)                                                                                                              aCGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAggcgg                                                                                                           <  1:120608/73‑1 (MQ=255)                                                                                                                     gaggagTGCTCCGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGGCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTGGGCTTccc                         <  8:5495/148‑1 (MQ=255)                                                                                                                               ccGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTGGGCTTCCCAGATCACCAg               >  3:23125/1‑148 (MQ=255)                                                                                                                                cGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTGGGCTTCCCAGATCACCAgc              <  7:152969/148‑1 (MQ=255)                                                                                                                                cGGCCCC‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTGGGCTTCCCAGATCACCAgc              <  7:12808/148‑1 (MQ=255)                                                                                                                                    ccc‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTGGGCTTCCCAGATCACCAGCGCGt          >  4:93733/1‑148 (MQ=255)                                                                                                                                      c‑TGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTGGGCTTccc                         >  5:99821/1‑131 (MQ=255)                                                                                                                                        tGCCCTTCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTGGGCTTCCCa                        <  6:99821/131‑1 (MQ=255)                                                                                                                                             ttCACAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTGGGCTTCCCAGATCACCAGCGCGTTCGGCAtg  >  2:181572/1‑148 (MQ=255)                                                                                                                                                 |                                                                                                                                                GCAGCGGGCGAATGACCTGGCGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCC‑TGCCCTTCATAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTGGGCTTCCCAGATCACCAGCGCGTTCGGCATG  >  NC_002947/4733303‑4733589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑