Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,875,109	G→A	M99I (ATG→ATA)	PP_4284 →	transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,875,109	0	G	A	100.0%	131.2 / NA	39	M99I (ATG→ATA)	PP_4284	transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/21);  total (18/21)

CATCATCTTCGTCAACCCCAACATCATGGCCGACGCCGGCATCGACCATGGTGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGTTGTTCATGTTCCTGACCTTGTCGAAAGTGCGCGAATGGCTGCTCAACA  >  NC_002947/4874965‑4875248                                                                                                                                                 |                                                                                                                                            catcatCTTCGTCAACCCCAACATCATGGCCGACGCCGGCATCGACCATGGTGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATaggg                                                                                                                                          >  5:57890/1‑148 (MQ=255)   tcatcTTCGTCAACCCCAACATCATGGCCGACGCCGGCATCGACCATGGTGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCt                                                                                                                                        <  1:81348/148‑1 (MQ=255)      tcTTCGTCAACCCCAACATCATGGCCGACGCCGGCATCGACCATGGTGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCaa                                                                                                                                     >  2:180883/1‑148 (MQ=255)       cTTCGTCAACCCCAACATCATGGCCGACGCCGGCATCGACCATGGTGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAAt                                                                                                                                    >  4:148276/1‑148 (MQ=255)         tCGTCAACCCCAACATCATGGCCGACGCCGGCATCGACCATGGTGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGc                                                                                                                                  >  8:50546/1‑148 (MQ=255)            tCAACTCCAACATCATGGCCGACGCCGGCATCGACCATGGTGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCctt                                                                                                                               >  1:54214/1‑148 (MQ=255)             cAACCCCAACATCATGGCCGACGCCGGCATCGACCATGGTGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCcttc                                                                                                                              <  2:167201/148‑1 (MQ=255)               aCCCCAACATCATGGCCGACGCCGGCATCGACCATGGTGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCcttct                                                                                                                             >  4:10515/1‑147 (MQ=255)                 cccAACATCATGGCCGACGCCGGCATCGACCATGGTGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTa                                                                                                                          >  6:79164/1‑148 (MQ=255)                   cAACATCATGGCCGACGCCGGCATCGACCATGGTGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTAcc                                                                                                                        <  3:176354/148‑1 (MQ=255)                     aCATCATGGCCGACGCCGGCATCGACCATGGTGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTa                                                                                                                      <  1:94336/148‑1 (MQ=255)                        tcatGGCCGACGCCGGCATCGACCATGGTGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTacac                                                                                                                   >  7:115032/1‑148 (MQ=255)                            ggCCGACGCCGGCATCAACCATGGTGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTg                                                                                                               <  1:91/148‑1 (MQ=255)                                            gACCATGGTGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCAGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCt                                                                                               >  2:118372/1‑148 (MQ=255)                                               cATGGTGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTGTACCTACACCGTGGTCGGCACCATGGGCTACa                                                                                            >  7:115991/1‑148 (MQ=255)                                                 tGGTGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAAc                                                                                          <  1:180883/148‑1 (MQ=255)                                                    tGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTgg                                                                                       <  5:28974/148‑1 (MQ=255)                                                    tGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCGGGGCTGCCTGCTGAGGGGCCTGTACGCCAACTGGCCGGGGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTgg                                                                                       <  7:79177/148‑1 (MQ=255)                                                             tGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACgg                                                                                <  3:148276/146‑1 (MQ=255)                                                                  ccACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTggg                                                                         >  5:94429/1‑148 (MQ=255)                                                                          aTCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTAt                                                                 >  1:98872/1‑148 (MQ=255)                                                                          aTCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTAt                                                                 <  5:79164/148‑1 (MQ=255)                                                                          aTCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTAt                                                                 >  8:164378/1‑148 (MQ=255)                                                                           tCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTAtt                                                                <  2:98872/148‑1 (MQ=255)                                                                            cgccgccgcGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACATCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTc                                                               <  3:17045/148‑1 (MQ=255)                                                                              ccgccgcGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTGTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGt                                                             >  5:157617/1‑148 (MQ=255)                                                                                     gcTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGc                                                      >  2:53213/1‑148 (MQ=255)                                                                                               ctgctgATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGTTGTTc                                             <  8:29555/147‑1 (MQ=255)                                                                                                tgctgATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATTCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGTTGTTCAt                                           <  4:94534/148‑1 (MQ=255)                                                                                                   tgATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGTTGTTCATg                                          <  7:50546/146‑1 (MQ=255)                                                                                                      tGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGTTGTTCATGTTc                                       <  8:153543/146‑1 (MQ=255)                                                                                                         gCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGAATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCTGGCGTGTTGTTCATGTTACTGAc                                  >  2:113208/1‑148 (MQ=255)                                                                                                            tGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTgg                                                                          >  3:84111/1‑105 (MQ=255)                                                                                                            tGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGTTGTTCATGTTCCTGACCtt                               >  7:136228/1‑148 (MQ=255)                                                                                                             gTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTggg                                                                         <  4:84111/105‑1 (MQ=255)                                                                                                               aCGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGTTGTTCATGTTCCTGACCTTGTc                            <  2:155466/148‑1 (MQ=255)                                                                                                                 gCCAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGTTGTTCATGTTCCTGACCTTGTCg                           >  6:54404/1‑147 (MQ=255)                                                                                                                   cAACTGGCCGGTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGTTGTTCATGTTCCTGACCTTGTCGaaa                        <  8:115032/148‑1 (MQ=255)                                                                                                                            ggTGGGCCTGGCGCCGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGTTGTTCATGTTCCTGACCTTGTCGAAAGTGCGCGaa               <  6:57890/148‑1 (MQ=255)                                                                                                                                         ccGGGCATAGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGTTGTTCATGTTCCTGACCTTGTCGAAAGTGCGCGAATGGCTGCTCAACa  <  2:54214/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                            CATCATCTTCGTCAACCCCAACATCATGGCCGACGCCGGCATCGACCATGGTGCCGCTTTTGTCGCCACCTGCATCGCCGCCGCGCTGGGCTGCCTGCTGATGGGCCTGTACGCCAACTGGCCGGTGGGCCTGGCGCCGGGCATGGGGCTCAATGCCTTCTTTACCTACACCGTGGTCGGCACCATGGGCTACAACTGGGAAACGGCGCTGGGGGCGGTATTCGTCTCGGGCGTGTTGTTCATGTTCCTGACCTTGTCGAAAGTGCGCGAATGGCTGCTCAACA  >  NC_002947/4874965‑4875248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑