Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	499,203	A→G	intergenic (+47/‑249)	uhpA → / → PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	499,203	0	A	G	100.0%	74.2 / NA	24	intergenic (+47/‑249)	uhpA/PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/12);  total (12/12)

TCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTAC  >  NC_002947/499065‑499341                                                                                                                                           |                                                                                                                                           tCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGccc                                                                                                                                   >  3:40798/1‑148 (MQ=255)  cAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAAGCCcg                                                                                                                                  >  1:110980/1‑148 (MQ=255)          acaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACggg                                                                                                                          >  8:110250/1‑148 (MQ=255)               gCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctgg                                                                                                                     >  2:20855/1‑148 (MQ=255)                   gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGTAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCTAACGGgcctggcctg                                                                                                                 <  5:154245/148‑1 (MQ=255)                                   gAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcct                                                                                                                       >  3:10049/1‑126 (MQ=255)                                    aaaGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctg                                                                                                                      <  4:10049/126‑1 (MQ=255)                                    aaaGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTg                                                                                                 <  6:87485/147‑1 (MQ=255)                                        tGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCg                                                                                            <  4:81603/148‑1 (MQ=255)                                                 tGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCAGCTGATCTCTATGGCTGACCCGGCCTCATcg                                                                                   >  4:57969/1‑148 (MQ=255)                                                    cGGAGCTGGTGACGTATGCCATGGAGCACAAGCTGATCTGATTGCGACATCCGGGGGCCCGAAACTTGCCGAAGCCTATGGGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg                                                                                <  1:149233/148‑1 (MQ=255)                                                       aGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATTCCCCCGCACCGGCCTGGCCTGCGGATCTAGATGGCGGCGCCGGCCGCGTAGCGGGCa                                                                             >  3:43972/1‑148 (MQ=255)                                                             tGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAAGCCCCCGAACCGGCCTGGCCAGCGGATCTCTATTGCGGCGCCCGGCGCATAGCGGGCAAGCCCg                                                                       >  7:115807/1‑148 (MQ=255)                                                                aGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGGGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGcga                                                                    <  8:87317/148‑3 (MQ=255)                                                                 gTACGCATTGGAGCACAAGCTGATCTTTTGGCCACATACGGGGGCCCTAACCTTGCCGACGCCTTTGTGGGCGCGGGCTTGCCCGCTAACGGGCCTGGCGTGCTGATCTCTATGGCTGAGCCGGCCGCTTCGCGGGAAGGCCCGCTc                                                                    <  2:47661/147‑1 (MQ=255)                                                                              cacaAGCTGATCTTTTGGCTACTAAGGGGTGCCCTAACCTTGCGGCCGCATATGTGGCAGGGGGCATGCCCGCTACCGGGCGTGGCCGGCTTATCTCTATGGGTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGt                                                       <  8:60437/147‑1 (MQ=16)                                                                                        tctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCCCGAACGGGCCTGGCCGGGGGAGCTCTATTGCGGAGCCCGCCGCTTAGCCGGGATGCCCGCGCCCCCACGGTTAgtgggtgggtag                                             >  2:17108/1‑143 (MQ=255)                                                                                          tgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCGCGATGGCTGCGCCGGCCACTTAGCGGGCATGCCCGCTCCCACAGGTTTCGTGGGTGGCTCGcca                                          >  8:124078/1‑146 (MQ=255)                                                                                          tgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACCGGCCTGGCCTGCTGAACTCTATGGCGTAGGCGGCCTCTTCGCGGGCCTGCCCGCTACCACAGGGTTAGTGGATGGCTCGCaa                                          >  8:121693/1‑148 (MQ=255)                                                                                                                     ttGCCGAAGCCTATGTGGTAGCGGGCATGCCCGCTAACGGGCCGGGCGTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTa               <  6:93217/148‑1 (MQ=39)                                                                                                                     ttGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCTAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTa               <  4:40798/148‑1 (MQ=255)                                                                                                                     ttGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGCACGGGGCGTGCAGGGTGCGGTATTTGGGTGAGGCGGGAGCTTAGCGGGCATTCACGCTCCCACAGGTTTCGTGGTTGGGGGGCAATGGCATTCCAAACCCGCCCTACATGTa               >  8:45881/1‑148 (MQ=255)                                                                                                                             gCCTTTGTGGGAGCGGGCATGCCCGCTAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATc       <  4:43972/148‑1 (MQ=255)                                                                                                                                     gggCGCGGGCATGCCCGCGAACGGGCCGGGCCTGCTGATCTCTTTGGGTGAGCCGGCCTGTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTac  <  7:110250/145‑1 (MQ=255)                                                                                                                                           |                                                                                                                                           TCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTAC  >  NC_002947/499065‑499341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑