Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,674,753	G→C	intergenic (+115/+25)	rhlE‑II → / ← yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,674,753	0	G	C	100.0%	112.9 / NA	36	intergenic (+115/+25)	rhlE‑II/yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (22/14);  total (22/14)

GCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑C‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAA  >  NC_002947/5674615‑5674888                                                                                                                                             |                                                                                                                                        gcgcTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGt                                                                                                                                 >  7:168687/1‑148 (MQ=255)     tgctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGttt                                                                                                                               >  5:80332/1‑146 (MQ=255)       ctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTCCTAGGGCCGCAAAGCC‑GCCCCCGCGGTTTTCAg                                                                                                                           >  1:71999/1‑148 (MQ=37)          aCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGATCCTGCAGAGAATTTCTTGATATCTACAGGATCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTg                                                                                                                        >  3:140585/1‑148 (MQ=37)              gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCGGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCa                                                                                                                     >  8:62818/1‑147 (MQ=255)                 aGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGaaa                                                                                                                 <  2:30084/148‑1 (MQ=255)                     cTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTAc                                                                                                             >  3:10804/1‑148 (MQ=255)                        aTAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTc                                                                                                          >  2:29876/1‑148 (MQ=255)                        aTAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTc                                                                                                          >  1:28683/1‑148 (MQ=255)                             aGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt                                                                                                     <  4:176799/148‑1 (MQ=255)                              ggaCCGAGGGGGCTGCTTCGCAGCCCTAGCACGACACCAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTc                                                                                                    <  3:37720/146‑1 (MQ=37)                                                 gCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGatat                                                                                 >  7:15698/1‑148 (MQ=255)                                                  cAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtc                                                                                <  4:137882/148‑1 (MQ=255)                                                   aGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTTGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGatat                                                                                 >  6:12375/1‑146 (MQ=255)                                                     cccTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag                                                                             <  6:80332/148‑1 (MQ=255)                                                     cccTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag                                                                             >  2:106904/1‑148 (MQ=255)                                                     cccTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag                                                                             <  4:10804/148‑1 (MQ=255)                                                          tCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATGTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGGTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtcca                                                                        >  5:157642/1‑148 (MQ=38)                                                            aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                      >  4:20361/1‑148 (MQ=255)                                                                        cGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTa                                                          <  2:131732/148‑1 (MQ=255)                                                                                cAGAGAATTTCTTGATCTCTATAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGGCTGCGAAGTAGCACCCgg                                                  >  5:60412/1‑148 (MQ=39)                                                                                 agagAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCggg                                                 >  6:133870/1‑148 (MQ=255)                                                                                 agagAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCggg                                                 >  1:26250/1‑148 (MQ=255)                                                                                 agagAATTTCTTGAGCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCgg                                                  <  3:87782/147‑1 (MQ=255)                                                                                           ttGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGc                                       >  8:98786/1‑148 (MQ=255)                                                                                                 tctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGt                                 >  2:48975/1‑148 (MQ=255)                                                                                                  ctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTc                                >  5:31280/1‑148 (MQ=255)                                                                                                    aCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                   <  1:40293/143‑1 (MQ=255)                                                                                                      aGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgt                              >  2:93125/1‑146 (MQ=255)                                                                                                        gAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcag                          >  4:63923/1‑148 (MQ=255)                                                                                                                   tgtCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgt                 <  2:26250/146‑1 (MQ=255)                                                                                                                      cGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtg             <  4:140585/147‑1 (MQ=255)                                                                                                                         gATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg          >  1:184428/1‑147 (MQ=255)                                                                                                                                ccGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa  <  5:36374/148‑1 (MQ=255)                                                                                                                                ccGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa  <  3:108407/148‑1 (MQ=255)                                                                                                                                ccGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa  <  6:176123/148‑1 (MQ=255)                                                                                                                                             |                                                                                                                                        GCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑C‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAA  >  NC_002947/5674615‑5674888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑