Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 563,783 T→C L289S (TTG→TCG)  rpoA → DNA‑directed RNA polymerase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947563,7830TC100.0% 150.9 / NA 44L289S (TTG→TCG) rpoADNA‑directed RNA polymerase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/27);  total (17/27)

TAGTCGAGCAGGAAGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTTGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAGACGACAAGGCGACCGCCTGATCGTCGTA  >  NC_002947/563639‑563927
                                                                                                                                                |                                                                                                                                                
tAGTCGAGCAGGAAGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGaa                                                                                                                                               <  4:174016/148‑1 (MQ=255)
  gTCGAGCAGGAAGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGa                                                                                                                                             >  7:67830/1‑148 (MQ=255)
      aGCAGGAAGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTc                                                                                                                                          <  1:118899/147‑1 (MQ=255)
          ggAAGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAAc                                                                                                                                     <  7:108083/148‑1 (MQ=255)
               aCGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGa                                                                                                                                                >  2:108935/1‑132 (MQ=255)
                agagaTCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGTCTTAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGt                                                                                                                               <  7:136937/147‑1 (MQ=255)
                     tcgatcCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTaagaa                                                                                                                          <  8:79994/148‑1 (MQ=255)
                         tccgatccTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTcc                                                                                                                      >  1:143550/1‑148 (MQ=255)
                          ccgatccTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTccc                                                                                                                     <  8:67830/148‑1 (MQ=255)
                             atccTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCctga                                                                                                                  <  4:169088/148‑1 (MQ=255)
                                                  gacCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTgg                                                                                             <  8:51389/148‑1 (MQ=255)
                                                       ggAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCctct                                                                                        >  6:124857/1‑148 (MQ=255)
                                                           ctgactgTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTg                                                                                    <  5:124857/148‑1 (MQ=255)
                                                              actgTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGgtc                                                                                 >  4:13868/1‑148 (MQ=255)
                                                               ctgTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGgtct                                                                                <  8:16687/148‑1 (MQ=255)
                                                               ctgTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGgtct                                                                                >  5:180399/1‑148 (MQ=255)
                                                               ctgTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGgt                                                                                  <  5:54412/146‑1 (MQ=255)
                                                                         ggCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGc                                                                      >  8:1481/1‑148 (MQ=255)
                                                                             aaCTGCCTCAAGGCTGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATgc                                                                  <  6:130990/148‑1 (MQ=255)
                                                                             aaCTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACt                                                                                                                                           >  5:132969/1‑75 (MQ=255)
                                                                             aaCTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATgc                                                                  >  3:114041/1‑148 (MQ=255)
                                                                              aCTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTc                                                                                                                                          <  6:132969/75‑1 (MQ=255)
                                                                                   cTCAAGGCGGAGACCATCTACTACATCGGCGACCTGATGCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCt                                                              <  5:144620/146‑1 (MQ=255)
                                                                                   cTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTc                                                             <  6:62222/147‑1 (MQ=255)
                                                                                   cTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTc                                                             <  8:121373/147‑1 (MQ=255)
                                                                                        ggCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAAc                                                       >  5:138066/1‑148 (MQ=255)
                                                                                           ggAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTgg                                                    >  8:126068/1‑148 (MQ=255)
                                                                                               aaCATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGgccgc                                                <  1:82842/148‑1 (MQ=255)
                                                                                                            tCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTaa                                   <  8:28988/148‑1 (MQ=255)
                                                                                                                cGACCTGATTCAGCGTACCGAAGTAGAGCGGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTaagaaa                               <  7:147176/148‑1 (MQ=255)
                                                                                                                   ccTGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAgac                            <  2:172606/148‑1 (MQ=255)
                                                                                                                     tGATTCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAgacg                           >  7:140756/1‑147 (MQ=255)
                                                                                                                         tCAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCt                                      >  4:102807/1‑132 (MQ=255)
                                                                                                                          cAGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCtt                                     <  3:102807/132‑1 (MQ=255)
                                                                                                                           aGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCt                                      >  7:124780/1‑130 (MQ=255)
                                                                                                                           aGCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAGACGACAAgg                     <  3:13868/147‑1 (MQ=255)
                                                                                                                            gCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCtt                                     <  8:124780/130‑1 (MQ=255)
                                                                                                                            gCGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTaagaa                                >  7:20875/1‑135 (MQ=255)
                                                                                                                             cGTACCGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTaagaaa                               <  8:20875/135‑1 (MQ=255)
                                                                                                                                  cGAAGTAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAGACGACAAGGCGACCGcc             >  2:131262/1‑148 (MQ=255)
                                                                                                                                          agCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAGACGACAAGGCGACCGCCTGAtcgtc     <  5:97551/148‑1 (MQ=255)
                                                                                                                                          agCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTGCTGGCCTCTCGTGGTCTGTCTCTCGGCAGGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAGACGACAAGGCGACCGCCTGAtcgtc     <  3:178307/148‑1 (MQ=255)
                                                                                                                                           gCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAGACGACAAGGCGACCGCCTGAtcgtcg    <  8:37088/148‑1 (MQ=255)
                                                                                                                                             tGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAGACGACAAGGCGACCGCCTGATCGTCGTa  >  5:81128/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TAGTCGAGCAGGAAGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCGAAGTAGAGCTGTTGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAGACGACAAGGCGACCGCCTGATCGTCGTA  >  NC_002947/563639‑563927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑