| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,497 | 1 | . | C | 76.9% | 20.3 / ‑3.0 | 15 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/2); major base C (3/7); minor base A (3/0); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,498 | 0 | T | G | 93.3% | 33.3 / ‑3.4 | 15 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (6/8); minor base C (0/1); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01 | |||||||||||
CTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCA‑C‑GGCTTTGCCGGTGT‑T‑TCGGG‑CACG‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGG‑CCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCT > NC_002947/1499391‑1499635 || cTGAACTACGCCTTGGCACAACAGCAGGAGTTCGGCTGCCAAAGGGTGGGTGCGGGCTTGCCCGCGAAACTGGCGCCGGGGTGCAGGGCAC‑C‑GGCTTGGCCGGTGT‑T‑CGGGG‑GAAGGCCCGCTCCCACAGACTGATTGCCATTAAGCg < 8:19764/148‑1 (MQ=255) aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGGCCCCGCAACCCGCGCCCCCGTGGAAGGCCC‑C‑CGCGTTGGCGGTGT‑TAGCGGG‑CACGC‑CCGCGACCCCAGCCGGATTGCCAATAAGCGGGc > 2:38057/1‑148 (MQ=255) aCGAAGCCTTGGCACCACAGCTGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGT‑TCGCGGG‑CACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGc < 2:26782/147‑1 (MQ=21) cagGAGTGAGGCGGCAAAAGGGGGGGCGCGGGCGGGCCCGCTAAACCGGCGCCGGGGGGCA‑GGCCA‑CGGGGTTTGCGGGTGT‑TCGGGGG‑CACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGG‑ccc < 2:28455/148‑1 (MQ=255) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCCCGCAACCGGCGCCGCGGTGCATGGCAC‑C‑GGCTTTTCCGGTGT‑TAGCGGG‑CCCGC‑CCGCTCCCCCAGCCGGAGTGGCATTAAGCGGGCCTATAGCTACAGTGACGG‑CCCGgtttttt > 8:60150/1‑147 (MQ=16) ggCTGCCAAACGGTGGGAGCGGGCGTGGCCCCGAAACAGGCGCCGCGGTGCCTTGCAC‑C‑CGCTTTTCCGGTGT‑TAGCGGGCCCCC‑‑CCGCGCCCACAGACAGATTTCCATTAAACCGGCCGATAGCGACACTGACGGCCCCGttttttt > 4:52705/1‑147 (MQ=255) ggTGGGAGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGT‑TCGCGGG‑CACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGG‑CCCGTTTTTTGTTGCCTCGCTaa < 4:166966/148‑1 (MQ=21) gCGGGGGTGCCCGCGAAACAGGCGCCGCGGTG‑ATGGGCACC‑GGCTTGGCGGGTTT‑TCGCGGG‑CACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGG‑CCCGTTTTTTGTTGCCTCGCTAACGAATCa < 6:17012/148‑1 (MQ=25) ggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGT‑TCGCGGG‑CACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGG‑CCCGTTTTTTGTTGCCTCGCTAACGAATCATGa < 8:141977/148‑1 (MQ=37) gTGCCCGCGAAACCGGCGCCGCGGTGCATGGCAC‑C‑GGCTTTGCCGGGGTGC‑GCGGG‑CACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGG‑CCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc < 1:132138/148‑1 (MQ=25) cccGCGAAACAGGCGCCGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGT‑TCGCGGG‑CACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGG‑CCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt < 3:111746/148‑1 (MQ=37) cgcgAAACAGGCGCCGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGT‑TCGCGGG‑CACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGG‑CCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc > 2:11582/1‑143 (MQ=37) gcgAAACAGGCGCCGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGT‑TCGCGGG‑CACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGG‑CCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc < 1:11582/143‑1 (MQ=37) aCAGGCGCCGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGT‑TCGCGGG‑CACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGG‑CCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg > 5:77372/1‑147 (MQ=37) ccGGTGT‑TCGCGGG‑CACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTCTCGCTACAGTGACGG‑CCCGGGTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCACGCAGATCTGAAACGTCCCGCctgct > 7:161504/1‑148 (MQ=255) || CTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCA‑C‑GGCTTTGCCGGTGT‑T‑TCGGG‑CACG‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGG‑CCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCT > NC_002947/1499391‑1499635 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |