Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,300,170	Δ1 bp	7.7%	intergenic (+68/+14)	pvdQ → / ← lpxH	acyl‑homoserine lactone acylase PvdQ/UDP‑2,3‑diacylglucosamine hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,300,170	0	G	.	7.7%	153.6 / 7.2	52	intergenic (+68/+14)	pvdQ/lpxH	acyl‑homoserine lactone acylase PvdQ/UDP‑2,3‑diacylglucosamine hydrolase
Reads supporting (aligned to +/- strand):  ref base G (24/23);  new base . (2/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTGCAAGGCCCAACCGCGGCGGTCCCAGTCGCCCAGCACGATACGCCGTGCCGGCTGGCCATCGACCATCAGCTTGTGAATCGCT  >  NC_002947/3300024‑3300313                                                                                                                                                    |                                                                                                                                                gcAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAg                                                                                                                                               <  1:247802/149‑1 (MQ=255)  cAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAgg                                                                                                                                              <  8:317671/149‑1 (MQ=255)  cAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAgg                                                                                                                                              >  8:180608/1‑149 (MQ=255)    gATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGcc                                                                                                                                            >  5:137913/1‑149 (MQ=255)         aGGCAGATCCTGAGTACCGGGAAGTGGTGATTAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGa                                                                                                                                                <  1:315715/140‑1 (MQ=255)         aGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGa                                                                                                                                                >  2:315715/1‑140 (MQ=255)           gaaGATCCTGAGTACCGGGAAGTGGTGATAAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGACCATTCGCGGGTAAAACAGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGa                                                                                                                                                >  7:142160/3‑138 (MQ=255)           gCAGATCCTGAGTACCGGGAAGTGGTGATCATTTAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGa                                                                                                                                                <  8:142160/138‑1 (MQ=255)            cataTCCTGAGTACCTGGAAGTGGTGCTCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAAGGGCCTCGCGGGCAAGCCCGC‑AGGccc                                                                                                                                           <  6:266290/139‑1 (MQ=38)            cAGAACCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAAGGGCCTCGCGGGCAAGCCCGC‑AGGccc                                                                                                                                           >  5:266290/1‑142 (MQ=38)              gATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTc                                                                                                                                  <  5:138151/149‑1 (MQ=255)                       tACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACt                                                                                                                         >  3:309670/1‑149 (MQ=255)                         ccGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGTGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCg                                                                                                                       <  5:266245/149‑1 (MQ=255)                         ccGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTc                                                                                                                        <  4:309670/148‑1 (MQ=255)                         ccGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCg                                                                                                                       <  3:126881/149‑1 (MQ=255)                            ggAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAAc                                                                                                                    <  7:220546/149‑1 (MQ=255)                                   gtgATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCt                                                                                                                                          >  3:174753/1‑120 (MQ=255)                                   gtgATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCt                                                                                                                                          <  4:174753/120‑1 (MQ=255)                                   gtgATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGcc                                                                                                              >  3:3725/1‑148 (MQ=255)                                   gtgATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGcc                                                                                                              >  3:8915/1‑148 (MQ=255)                                     gATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAAc                                                                                                           >  7:230408/1‑149 (MQ=255)                                         aGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGa                                                                                                        <  8:118171/148‑1 (MQ=255)                                            gAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGc                                                                                                    >  4:8534/1‑149 (MQ=255)                                               cGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCTCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCt                                                                                                 <  1:239325/149‑1 (MQ=255)                                                  gACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGtt                                                                                              <  3:183900/149‑1 (MQ=255)                                                    cAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGt                                                                                               >  1:149847/1‑146 (MQ=255)                                                     aaGGGGGCGGTAGTCAGCCAGCCCTGAGGGGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCg                                                                                            >  8:108197/1‑148 (MQ=255)                                                       gggggCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCa                                                                                         <  8:230408/149‑1 (MQ=255)                                                        ggggCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCTAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCaa                                                                                        <  3:160604/149‑1 (MQ=255)                                                                 gTCAGCCAGCCCTGAGGGGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTGCAAg                                                                                <  7:108197/148‑1 (MQ=255)                                                                      ccagccCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTGCAAGGCCCaa                                                                          >  5:355979/1‑149 (MQ=255)                                                                                        ccTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTGCAAGGCCCAACCGCGGCGGTCCCAGTCg                                                        >  8:256192/1‑149 (MQ=255)                                                                                         cTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAg                                                                                                                                <  1:111921/76‑1 (MQ=255)                                                                                         cTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAg                                                                                                                                >  2:111921/1‑76 (MQ=255)                                                                                           gcgcCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTGCAAGGCCCAACCGCGGCGGTCCCAGTCGcc                                                      >  8:263230/1‑148 (MQ=255)                                                                                             gcCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCAT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGGACGTCAACCTCCAAGGCCCAACCCCGGCGGACCCAGTCCCCCAg                                                   >  6:245030/1‑149 (MQ=39)                                                                                               cGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTGCAAGGCCCAACCGCGGCGGTCCCAGTCGCCCAGCa                                                 <  6:355979/149‑1 (MQ=255)                                                                                                   ccATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCa                                                                                                                                 >  1:252068/1‑65 (MQ=255)                                                                                                   ccATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCa                                                                                                                                 <  2:252068/65‑1 (MQ=255)                                                                                                     aTTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTGCAAGGCCCAACCGCGGCGGTCCCAGTCGCCCAGCACGATAc                                           >  3:189418/1‑149 (MQ=255)                                                                                                          cgGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTGCAAGGCCCAACCGCGGCGGTCCCAGTCGCCCAGCACGATACGCCGt                                      <  5:245030/149‑1 (MQ=255)                                                                                                              tAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTg                                                                                    >  3:151586/1‑99 (MQ=255)                                                                                                              tAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTg                                                                                    <  4:151586/99‑1 (MQ=255)                                                                                                                             aGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTGCAAGGCCCAACCGCGGCGGTCCCAGTCGCCCAGCACGATACGCCGTGCCGGCTGGCCATCGACCa                   >  5:217337/1‑149 (MQ=255)                                                                                                                                  ccT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTGCAAGGCCCAACCGCGGCGGTCCCAGTCGCCCAGCACGATACGCCGTGCCGGCTGGCCATCGACCATCAGc              >  2:301549/1‑149 (MQ=255)                                                                                                                                   cT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTGCAAGGCCCAACCGCGGCGGTCCCAGTCGCCCAGCACGATACGCCGTGCCGGCTGGCCATCGACCATCAGCt             <  2:112719/149‑1 (MQ=255)                                                                                                                                   cT‑GCGGGCTTGCCCGC‑AGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTGCAAGGCCCAACCGCGGCGGTCCCAGTCGCCCAGCACGATACGCCGTGCCGGCTGGc                            <  6:192556/133‑1 (MQ=255)                                                                                                                                   cT‑GCGGGCTTGCCCGC‑AGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTGCAAGGCCCAACCGCGGCGGTCCCAGTCGCCCAGCACGATACGCCGTGCCGGCTGGc                            >  5:192556/1‑133 (MQ=255)                                                                                                                                       cGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTGCAAGGCCCAACCGCGGCGGTCCCAGTCGCCCAGCACGATACGCCGTGCCGGCTGGCCATCGACCATCAGCtt            <  7:263230/147‑1 (MQ=255)                                                                                                                                       cGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTGCAAGGCCCAACCGCGGCGGTCCCAGTCGCCCAGCACGATACGCCGTGCCGGCTGGCCATCGACCATCAGCTtgt          <  5:220813/149‑1 (MQ=255)                                                                                                                                        gggCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTGCAAGGCCCAACCGCGGCGGTCCCAGTCGCCCAGCACGATACGCCGTGCCGGCTGGCCATCGACCATCAGCTtgt          >  3:137548/1‑148 (MQ=255)                                                                                                                                               cccGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTGCAAGGCCCAACCGCGGCGGTCCCAGTCGCCCAGCACGATACGCCGTGCCGGCTGGCCATCGACCATCAGCTTGTGAATCGCt  >  2:369928/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                GCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCCTGCGCCGGCCCATTCGCGGGTAAACCCGCTCCTACAGGGGCCT‑GCGGGCTTGCCCGCGAGGCCCTGTAATGTCAGGAAAACTCGAACGGCGCCAACTGAAAGCCCTGTTCGTCAACCTGCAAGGCCCAACCGCGGCGGTCCCAGTCGCCCAGCACGATACGCCGTGCCGGCTGGCCATCGACCATCAGCTTGTGAATCGCT  >  NC_002947/3300024‑3300313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑