Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,625,544 Δ1 bp 9.1% intergenic (‑57/+20) PP_3195 ← / ← PP_3196 hypothetical protein/nodulation protein d1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,625,5440T. 9.1% 119.7 / 7.1 44intergenic (‑57/+20)PP_3195/PP_3196hypothetical protein/nodulation protein d1
Reads supporting (aligned to +/- strand):  ref base T (22/18);  new base . (2/2);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TCGTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGC  >  NC_002947/3625402‑3625690
                                                                                                                                               |                                                                                                                                                  
tCGTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAGACACCGCAGCGGTG‑CTgggg                                                                                                                                              <  1:132364/149‑1 (MQ=255)
               cccACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCt                                                                                                                                >  8:180433/1‑148 (MQ=255)
               cccACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTc                                                                                                                               <  1:230708/149‑1 (MQ=255)
               cccACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGAACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTc                                                                                                                               <  2:321004/149‑1 (MQ=255)
                     tGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCgg                                                                                                                         >  6:107672/1‑149 (MQ=255)
                     tGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCg                                                                                                                          <  8:124518/148‑1 (MQ=255)
                           aCAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGgcgc                                                                                                                   <  7:180433/149‑1 (MQ=255)
                           aCAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGgcgc                                                                                                                   <  3:118213/149‑1 (MQ=255)
                                          gcgGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCAGGCt                                                                                                    >  3:188416/1‑149 (MQ=255)
                                                          gTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACt                                                                                     >  4:239452/1‑148 (MQ=255)
                                                              gcaggcagGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAgg                                                                                                                            <  8:262952/105‑1 (MQ=255)
                                                              gcaggcagGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAgg                                                                                                                            >  7:262952/1‑105 (MQ=255)
                                                              gcaggcagGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATg                                                                                <  5:107672/149‑1 (MQ=255)
                                                                  gcagggcaggGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTCTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAt                                                                            >  7:216862/1‑149 (MQ=255)
                                                                            gTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGAc                                                                              <  4:218539/137‑1 (MQ=255)
                                                                            gTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGAc                                                                              >  3:218539/1‑137 (MQ=255)
                                                                                 gCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCTTCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTcgc                                                             >  5:263145/1‑149 (MQ=255)
                                                                                 gCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTcgc                                                             >  4:232894/1‑149 (MQ=255)
                                                                                     ttcttGATCTGTGATGCGTGGTATCGCGAAACGCGATGCCGCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGGCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAg                                                         <  4:188416/145‑1 (MQ=255)
                                                                                                     cGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCTTCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATggg                                          <  6:263145/148‑1 (MQ=255)
                                                                                                      gAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCCGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGt                                        >  4:294254/1‑149 (MQ=255)
                                                                                                      gAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCAGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGt                                        >  2:163487/1‑149 (MQ=255)
                                                                                                         gTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGc                                     >  4:344078/1‑149 (MQ=255)
                                                                                                           aTCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAACGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCg                                   >  5:325976/1‑149 (MQ=255)
                                                                                                            tCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGt                                  <  3:344078/149‑1 (MQ=255)
                                                                                                            tCGCGAAACGCGATGCCCCAG‑CACCGCTGCCGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGt                                  >  1:30670/1‑149 (MQ=255)
                                                                                                              gcgAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTcc                                <  8:231364/149‑1 (MQ=255)
                                                                                                              gcgAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTc                                 >  7:231364/1‑148 (MQ=255)
                                                                                                               cgAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCCCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTcc                                >  4:309054/1‑148 (MQ=255)
                                                                                                                    cgcgATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCAtgctg                          >  7:276256/1‑149 (MQ=255)
                                                                                                                       atcgCCCCAGACACCGCAGCGGTG‑CTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCgg                       >  1:63577/4‑149 (MQ=255)
                                                                                                                           ccccAGACACCGCAGCGGCG‑CTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGc                    >  8:153295/1‑148 (MQ=255)
                                                                                                                            cccAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                  >  5:52926/1‑149 (MQ=255)
                                                                                                                             ccAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                  >  1:304593/1‑148 (MQ=255)
                                                                                                                                 acACCGCAGCGGCG‑CTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCa             <  7:153295/148‑1 (MQ=255)
                                                                                                                                  cACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAAt                                                                                 >  6:137777/1‑81 (MQ=255)
                                                                                                                                  cACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAAt                                                                                 <  5:137777/81‑1 (MQ=255)
                                                                                                                                   aCCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCCGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCAt            <  3:294254/149‑1 (MQ=255)
                                                                                                                                     cGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGa          <  2:30670/149‑1 (MQ=255)
                                                                                                                                       cTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAAt        >  7:310719/1‑149 (MQ=255)
                                                                                                                                        tGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAAtt       <  8:276256/149‑1 (MQ=255)
                                                                                                                                        tGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAAt        >  3:31600/1‑148 (MQ=255)
                                                                                                                                          cGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAAtt       <  3:232894/147‑1 (MQ=255)
                                                                                                                                           ggTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCAc    <  6:325976/149‑1 (MQ=255)
                                                                                                                                            gtgtCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACg   >  7:130331/1‑149 (MQ=255)
                                                                                                                                             tgtCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACg   >  7:38324/1‑148 (MQ=255)
                                                                                                                                             tgtCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGc  <  3:309054/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
TCGTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGC  >  NC_002947/3625402‑3625690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑