Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,273,045	Δ1 bp	8.3%	coding (192/1125 nt)	rlmG →	ribosomal RNA large subunit methyltransferase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,273,045	0	A	.	8.3%	187.8 / 11.2	60	coding (192/1125 nt)	rlmG	ribosomal RNA large subunit methyltransferase G
Reads supporting (aligned to +/- strand):  ref base A (23/32);  new base . (2/3);  total (25/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AGCCGGAGCAGGCCAACGACCCGTTGCAGGCCTTCGATGCCGCCGACGAGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAGTGTGCCGTTCGTACCGGCCAGCGAACACTGGCAAGGCCCGTTCGACCGGGTGCTGG  >  NC_002947/5272900‑5273193                                                                                                                                                   |                                                                                                                                                     aGCCGGAGCAGGCCAACGACCCGTTGCAGGCCTTCGATGCCGCCGACGAGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGc                                                                                                                                                   <  7:91147/149‑1 (MQ=255) aGCCGGAGCAGGCCAACGACCCGTTGCAGGCCTTCGATGCCGCCGACGAGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGc                                                                                                                                                   <  2:138319/149‑1 (MQ=255)   ccGGAGCAGGCCAACGACCCGTTGCAGGCCTTCGATGCCGCCGACGAGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCa                                                                                                                                                 <  5:378664/149‑1 (MQ=255)   ccGCAGCAGGCCACCCCCCCGTTCCAGCCCTTCGATGCCGCCTACGAGTCCCTGCTTGCGCTGCTCCACGAGCAGGCACCCTACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCa                                                                                                                                                 <  1:288789/149‑1 (MQ=255)     ggAGCAGGCCAACGACCCGTTGCAGGCCTTCGATGCCGCCGACGAGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCCTGCAAGGCTTGC‑GGCCAgg                                                                                                                                               <  5:206582/149‑2 (MQ=255)          aGGCGAACGACCCGTTGCAGGCCTTCGATGACGCCGACGAGTACCTGCTTGGGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAg                                                                                                                                          <  4:121543/149‑1 (MQ=255)              cAACGACCCGTTGCAGGCCTTCGATGCCGCCGACGAGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAggtgg                                                                                                                                      <  1:19073/149‑1 (MQ=255)              cAACGACCCGTTGCAGGCCTTCGATGCCGCCGACGAGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAggtgg                                                                                                                                      >  2:260559/1‑149 (MQ=255)                 cGACCCGTTGCAGGCCTTCGATGCCGCCGACGAGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTca                                                                                                                                   <  3:52799/149‑1 (MQ=255)                  gACCCGTTGCAGGCCTTCGATGCCGCCGACGAGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTcag                                                                                                                                  >  8:65044/1‑149 (MQ=255)                        ttGCAGGCCTTCGATGCCGCCGACGAGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCgg                                                                                                                            <  7:65044/149‑1 (MQ=255)                          gCAGGCCTTCGATGCCGCCGACGAGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGt                                                                                                                           <  8:46752/148‑1 (MQ=255)                              gCCTTCGCTGCCGCCGACGAGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTc                                                                                                                      <  7:239724/149‑1 (MQ=255)                              gCCTTCGATGCCGCCGACGAGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTc                                                                                                                      >  3:30611/1‑149 (MQ=255)                                cTTCGATGCCGCCGACGAGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAgg                                                                                                                                                    >  4:169090/1‑117 (MQ=255)                                         cgccgACGAGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGc                                                                                                           <  4:95417/149‑1 (MQ=255)                                            cgacgaGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCCTGCAAGGCTTGC‑GGCCAgggcac                                                                                                                                           <  6:278692/114‑6 (MQ=38)                                            cgacgaGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCCTGCAAGGCTTGC‑GGCCAgggcac                                                                                                                                           >  5:278692/1‑109 (MQ=38)                                                gaGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGc                                                                                                    >  6:212119/1‑149 (MQ=255)                                                  gTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCa                                                                                                                                                      >  3:195005/1‑97 (MQ=255)                                                  gTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCa                                                                                                                                                      <  4:195005/97‑1 (MQ=255)                                                  gTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGccc                                                                                                  <  2:174756/149‑1 (MQ=255)                                                    aCCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCAc                                                                                                         >  3:370813/1‑140 (MQ=255)                                                    aCCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCAc                                                                                                         <  4:370813/140‑1 (MQ=255)                                                      cTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTgg                                                                                               >  5:289964/1‑148 (MQ=255)                                                        gCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTcag                                                                                                                                  <  4:103273/111‑1 (MQ=255)                                                        gCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTcag                                                                                                                                  >  3:103273/1‑111 (MQ=255)                                                        gCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGaa                                                                                             >  5:205989/1‑148 (MQ=255)                                                               cAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTg                                                                                                <  8:334830/138‑1 (MQ=255)                                                               cAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTg                                                                                                >  7:334830/1‑138 (MQ=255)                                                                aGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTg                                                                                    >  5:267088/1‑149 (MQ=255)                                                                      acGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGgcgcg                                                                               >  7:267164/1‑148 (MQ=255)                                                                        gATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTg                                                                                                               >  6:254105/1‑114 (MQ=255)                                                                        gATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTg                                                                                                               <  5:254105/114‑1 (MQ=255)                                                                            aGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGc                                                                        >  4:305004/1‑149 (MQ=255)                                                                               ctcccGACGTCAATTTCCGGGTACTGGTGCTCATTGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGGACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCGTg                                                                     <  4:270265/147‑1 (MQ=255)                                                                               cccccGACGCCCATTGCCGGGGACTGGTGCTCACTGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGGCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTg                                                                     <  4:275268/147‑1 (MQ=255)                                                                                aCCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGc                                                                    >  6:39536/1‑149 (MQ=255)                                                                                   cGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGt                                                                 <  1:337068/149‑1 (MQ=255)                                                                                       gCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGa                                                             >  7:76371/1‑149 (MQ=255)                                                                                         cAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGa                                                             >  5:332709/1‑147 (MQ=255)                                                                                         cAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGa                                                             >  6:116558/1‑147 (MQ=255)                                                                                          aaTTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAg                                                          >  2:50127/1‑149 (MQ=255)                                                                                              gCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAgtgt                                                       <  2:229040/148‑1 (MQ=255)                                                                                               ccGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAGtgtg                                                      <  1:50127/148‑1 (MQ=255)                                                                                               ccGGGGACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAGtgtg                                                      <  6:267088/148‑1 (MQ=255)                                                                                                 gggTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAGTGTGCCg                                                   <  3:305004/149‑1 (MQ=255)                                                                                                   gTACTGGTGCTCAATGACAGCTTTGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGtt                                                                <  2:344604/134‑1 (MQ=255)                                                                                                   gTACTGGTGCTCAATGACAGCTTTGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGtt                                                                >  1:344604/1‑134 (MQ=255)                                                                                                    tACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAGTGTGCCGTTc                                                <  6:289964/149‑1 (MQ=255)                                                                                                               aaTGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAGTGTGCCGTTCGTACCGGCCAg                                     <  6:205989/149‑1 (MQ=255)                                                                                                                     aGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGcc                                                                   <  5:38093/113‑1 (MQ=255)                                                                                                                     aGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGcc                                                                   >  6:38093/1‑113 (MQ=255)                                                                                                                          cGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAGTGTGCCGTTCGTACCGGCCAGCGAACACTg                            <  5:212119/147‑1 (MQ=255)                                                                                                                            gTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAGTGTGCCGTTCGTACCGGCCAGCGAACACTGGCaa                        <  6:332709/149‑1 (MQ=255)                                                                                                                                       tgCAAGGCTTGC‑GGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAGTGTGCCGTTCGTACCGGCCAGCGAACACt                             <  4:47167/132‑1 (MQ=255)                                                                                                                                       tgCAAGGCTTGC‑GGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAGTGTGCCGTTCGTACCGGCCAGCGAACACt                             >  3:47167/2‑133 (MQ=255)                                                                                                                                         cAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAGTGTGCCGTTCGTACCGGCCAGCGAACACTGGCAAGGc                     >  3:259210/1‑140 (MQ=255)                                                                                                                                         cAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAGTGTGCCGTTCGTACCGGCCAGCGAACACTGGCAAGGc                     <  4:259210/140‑1 (MQ=255)                                                                                                                                             ccTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAGTGTGCCGTTCGTACCGGCCAGCGAACACTGGCAAGGCCCGTTCGACCggg        <  8:76371/149‑1 (MQ=255)                                                                                                                                              cTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGTGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAGTGTGCCGTTCGTACCGGCCAGCGAACACTGGCAAGGCCCGTTCGACCGGGt       >  1:270149/1‑149 (MQ=255)                                                                                                                                                  cAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAGTGTGCCGTTCGTACCGGCCAGCGAACACTGGCAAGGCCCGTTCGACCGGGTGCt    <  1:227903/148‑1 (MQ=255)                                                                                                                                                   aGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAGTGTGCCGTTCGTACCGGCCAGCGAACACTGGCAAGGCCCGTTCGACCGGGTGCTgg  >  7:96603/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     AGCCGGAGCAGGCCAACGACCCGTTGCAGGCCTTCGATGCCGCCGACGAGTACCTGCTTGCGCAGCTACACGATCAGGCACCCGACGCCAATTGCCGGGTACTGGTGCTCAATGACAGCTTCGGTGCCCTGGCC‑GCAAGCCTTGCAGGCCAGCTGCAGGTGGTCAGCAGCGGTGACTCGCACCTGGGGCACCTGGCCCTGGAAAAGAACCTGGCGCGCAATGGCCTGCCGTTCGACAGTGTGCCGTTCGTACCGGCCAGCGAACACTGGCAAGGCCCGTTCGACCGGGTGCTGG  >  NC_002947/5272900‑5273193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑