Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,962,569 Δ1 bp 7.9% coding (832/1833 nt) PP_1760 → ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,962,5690G. 7.9% 202.4 / 11.0 63coding (832/1833 nt)PP_1760ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (26/32);  new base . (2/3);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

ACCGCGCGCTTTACCCAGGCGCTAGCGGAAACCCTCGATGCCATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGACCTGTCGATCGGCCAGATGCTGGCGGTGTTCAGCTACCTGTGGTTCATGATCGGTCC  >  NC_002947/1962422‑1962714
                                                                                                                                                   |                                                                                                                                                  
aCCGCGCGCTTTACCCAGGCGCTAGCGGAAACCCTCGATGCCATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGa                                                                                                                                                   <  1:123200/149‑1 (MQ=255)
 ccGCGCGCTTTACCCAGGCGCTAGCGGAAACCCTCGATGCCATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGAt                                                                                                                                                  <  2:160513/149‑1 (MQ=255)
   gcgcgcTTTACCCAGGCGCTAGCGGAAACCCTCGATGCCATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGc                                                                                                                                                <  5:144018/149‑1 (MQ=255)
       gcTTTACCCAGGCGCTAGCGGAAACCCTCGATGCCATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGt                                                                                                                                            >  2:242703/1‑149 (MQ=255)
            aCCCAGGCGCTAGCGGAAACCCTCGATGCCATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGcc                                                                                                                                        <  3:58863/148‑1 (MQ=255)
               ccggCGCTAGCGGAAACCCTCGATGCCATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCGGGGCTTGCGGGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGc                                                                                                                                    <  8:309942/147‑1 (MQ=255)
                  gcgcTAGCGGAAACCCTCGATGCCATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAg                                                                                                                                 <  7:115087/149‑1 (MQ=255)
                    gcTAGCGGAAACCCTCGATGCCATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTg                                                                                                                               >  7:121990/1‑149 (MQ=255)
                      tAGCGGAAACCCTCGATGCCATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAACAGC‑AGGCCACTGGCACGGCCACTGGca                                                                                                                            >  6:144075/1‑148 (MQ=255)
                        gCGGAAACCCTCGATGCCATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAACAGC‑AGGCCACTGGCACGGCCACTGGcatc                                                                                                                          >  1:227694/1‑146 (MQ=38)
                         cGGAAACCCTCGATGCCATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCct‑g                                                                                                                         <  8:60335/149‑1 (MQ=255)
                           gAAACCCTCGATGCCATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCct‑gct                                                                                                                       >  6:85867/1‑149 (MQ=255)
                             tttccTGGATGGCATCGAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACACCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGt                                                                                                                     >  4:217737/4‑149 (MQ=255)
                              aCCCTCGATGCCATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGtt                                                                                                                    >  7:287223/1‑149 (MQ=255)
                                ccTCGATGCCATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTc                                                                                                                   >  7:250099/1‑148 (MQ=255)
                                    gATGCCATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAACAGC‑AGGCCACTGGCACGGCCACTGGCATCGCTTTTCCattg                                                                                                              <  8:299880/149‑4 (MQ=25)
                                    gATGCCATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAACAGC‑AGGCCACTGGCACGGCCACTGGCATCGCTTTTCCatt                                                                                                               <  2:227694/148‑3 (MQ=25)
                                        ccATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAACAGC‑AGGCCACTGGCACGGCCACTGGCATCGCTTTTCCattgcga                                                                                                           <  5:144075/148‑7 (MQ=21)
                                         cATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCg                                                                                                            <  2:17591/146‑1 (MQ=255)
                                         cATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCg                                                                                                            >  1:17591/1‑146 (MQ=255)
                                            ccAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCAGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCg                                                                                                      <  7:362637/149‑1 (MQ=255)
                                               ggAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTgg                                                                                                                              <  4:272905/123‑1 (MQ=255)
                                               ggAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTgg                                                                                                                              >  3:272905/1‑123 (MQ=255)
                                               ggAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTg                                                                                                                               >  4:42050/1‑122 (MQ=255)
                                                  aaTCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGATCGGCATCGACATc                                                                                                 <  8:250099/148‑1 (MQ=255)
                                                   aTCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACAt                                                                                                  <  7:194743/146‑1 (MQ=255)
                                                   aTCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACAt                                                                                                  >  8:194743/1‑146 (MQ=255)
                                                   aTCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCtt                                                                                               <  7:186254/149‑1 (MQ=255)
                                                   aTCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCtt                                                                                               <  1:242703/149‑1 (MQ=255)
                                                               aaCCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCggcggc                                                                                   >  2:87521/1‑149 (MQ=255)
                                                               aaCCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCggcggc                                                                                   >  1:101096/1‑149 (MQ=255)
                                                                   gCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATg                                                                               >  1:118354/1‑149 (MQ=255)
                                                                                ccTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGAACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTg                                                                   <  7:206714/148‑1 (MQ=255)
                                                                                ccTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACACCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTg                                                                   <  3:217737/148‑1 (MQ=255)
                                                                                 cTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGtt                                                                 <  2:258801/149‑1 (MQ=255)
                                                                                     gccgccTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTcc                                                             >  4:131103/1‑149 (MQ=255)
                                                                                     gccgccTGGGCGTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTc                                                              <  6:365635/148‑1 (MQ=255)
                                                                                      ccgccTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGtttt                                                               >  1:238403/1‑146 (MQ=255)
                                                                                      ccgccTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGtttt                                                               <  2:238403/146‑1 (MQ=255)
                                                                                       cgccTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGa                                                           <  1:87521/149‑1 (MQ=255)
                                                                                       cgccTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGa                                                           <  4:207051/149‑1 (MQ=255)
                                                                                        gccTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGAc                                                          <  5:85867/149‑1 (MQ=255)
                                                                                        gccTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGAc                                                          >  8:330857/1‑149 (MQ=255)
                                                                                          cTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGACCt                                                        <  8:121990/149‑1 (MQ=255)
                                                                                             ggCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGACCTGt                                                      <  8:287223/148‑1 (MQ=255)
                                                                                                       cccGCGAGGTGCGCGATTACGCCGTGGAGTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGAc                                                          >  1:215822/1‑134 (MQ=255)
                                                                                                       cccGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGAc                                                          <  2:215822/134‑1 (MQ=255)
                                                                                                       cccGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGACCTGTCGATCGGCCa                                            <  3:131103/148‑1 (MQ=255)
                                                                                                        ccGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCt                                                                    >  4:48522/1‑123 (MQ=255)
                                                                                                        ccGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGACCTGTCGATCGGc                                              >  3:44801/1‑145 (MQ=255)
                                                                                                             aGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGACCTGTCGATCGGCCAGATGCTg                                     <  4:44801/149‑1 (MQ=255)
                                                                                                                tGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATc                                                                                                       <  6:51571/80‑1 (MQ=255)
                                                                                                                tGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATc                                                                                                       >  5:51571/1‑80 (MQ=255)
                                                                                                                     gATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTGCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGAGCGTGCTGTTTTCCGACCTGTCGATCGGCCAGATGCTGGCGgtgt                              >  7:329610/1‑148 (MQ=255)
                                                                                                                      aTTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGACCTGTCGATCGGCCAGATGCTGGCGGTGTTc                            <  2:101096/149‑1 (MQ=255)
                                                                                                                      aTTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGACCTGTCGATCGGCCAGATGCTGGCGGTGTTc                            <  2:55695/149‑1 (MQ=255)
                                                                                                                         aCGCCGTGTACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGACCTGTCGATCGGCCAGATGCTGGCGGTGTTCAg                          <  2:118354/148‑1 (MQ=255)
                                                                                                                             cGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGACCTGTCGATCGGCCAGATGCTGGCGGTGTTCAGCTAcc                     >  2:173322/1‑149 (MQ=255)
                                                                                                                                       cAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGACCTGTCGATCGGCCAGATGCTGGCGGTGTTCAGCTACCTGTGGTTCAt           >  7:6465/1‑149 (MQ=255)
                                                                                                                                        aaTGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGACCTGTCGATCGGCCAGATGCTGGCGGTGTTCAGCTACCTGTGGTTCAt           >  3:150503/1‑148 (MQ=255)
                                                                                                                                         aTGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGACCTGTCGATCGGCCAGATGCTGGCGGTGTTCAGCTACCTGTGGTTCATGa         >  7:10160/1‑149 (MQ=255)
                                                                                                                                              aaaGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGACCTGTCGATCGGCCAGATGCTGGCGGTGTTCAGCTACCTGTGGTTCATGATCGGt    >  5:274603/1‑149 (MQ=255)
                                                                                                                                                aGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGACCTGTCGATCGGCCAGATGCTGGCGGTGTTCAGCTACCTGTGGTTCATGATCGGTcc  <  7:330857/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACCGCGCGCTTTACCCAGGCGCTAGCGGAAACCCTCGATGCCATCCAGGAAATCCGCGCCGGCAACCGCCAGGGCTACTTCCTTGGCCGCCTGGGCTTGCGTGCCCGCGAGGTGCGCGATTACGCCGTGGACTCGCAATGGAAAAGCGATGCCAGTGGCCGTGCCAGTGGCCT‑GCTGTTCCAGTTCGGCATCGACATCTTCCGCGCGGCGGCCATGCTGACCGTGCTGTTTTCCGACCTGTCGATCGGCCAGATGCTGGCGGTGTTCAGCTACCTGTGGTTCATGATCGGTCC  >  NC_002947/1962422‑1962714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑