Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,025,706	Δ1 bp	7.4%	coding (706/1110 nt)	wbpZ →	glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,025,706	0	A	.	7.4%	214.5 / 10.6	68	coding (706/1110 nt)	wbpZ	glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (28/35);  new base . (3/2);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

TGGCGCGCACGCATGAGCGAGCGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTCTGCTG  >  NC_002947/2025562‑2025852                                                                                                                                                 |                                                                                                                                                    tGGCGCGCACGCATGAGCGAGCGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAag                                                                                                                                                   >  1:160148/1‑147 (MQ=255)   gcgcgcACGCATGAGCGAGCGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAagcag                                                                                                                                                >  4:158769/1‑148 (MQ=255)    cgcgcACGCATGAGCGAGCGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAgcagca                                                                                                                                              <  3:158769/149‑1 (MQ=255)             aggagagagcTCTTTTTCCTGTTTGTATGCGCGTTGCGTTACTACAAGGGGCTTGATTACCTGCTAGAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGa                                                                                                                                    <  4:233660/147‑1 (MQ=255)             aTGAGCGAGCGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGa                                                                                                                                    <  4:289352/149‑1 (MQ=255)                  cgagcgCTTTTTGCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGATGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTC‑AGCAGCCTGCTGCTTGAGCtg                                                                                                                                <  2:266821/148‑2 (MQ=255)                  cgagcgCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCa                                                                                                                               <  3:303525/149‑1 (MQ=255)                  cgagcgCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTC‑AGCAGCCTGCTGCTTGAGCtgt                                                                                                                               <  6:171502/149‑3 (MQ=255)                    agcgCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGgctg                                                                                                                                         <  8:70084/138‑1 (MQ=255)                    agcgCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGgctg                                                                                                                                         >  7:70084/1‑138 (MQ=255)                      cgcTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGCGCTCAGGCt                                                                                                                           >  7:300660/1‑149 (MQ=255)                       gcTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGATAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCtt                                                                                                                          <  8:362050/149‑1 (MQ=255)                         tttttCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGa                                                                                                                        >  6:72142/1‑149 (MQ=255)                               cTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAg                                                                                                                                   <  7:83540/132‑1 (MQ=255)                                tGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGtt                                                                                                                 >  4:363220/1‑149 (MQ=255)                                 gTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGt                                                                                                                  <  8:154961/147‑1 (MQ=255)                                 gTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGt                                                                                                                  >  7:154961/1‑147 (MQ=255)                                  tttGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCa                                                                                                               <  5:72142/149‑1 (MQ=255)                                  tttGTAGGCGCGTGGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTc                                                                                                                <  2:299468/148‑1 (MQ=255)                                     gTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCCGGCTTGACAATGTTCAGtt                                                                                                            >  2:332576/1‑149 (MQ=255)                                           gcgTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTg                                                                                                        >  2:235930/1‑147 (MQ=255)                                           gcgTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTg                                                                                                        <  1:235930/147‑1 (MQ=255)                                           gcgTTGCGGTACTACAAGGGCCTGGATTCCCTGCTACAGGCTGCGTCTATCAACCGGCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTggg                                                                                                      <  4:230270/149‑1 (MQ=255)                                                gCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGc                                                                                                  <  7:23574/148‑1 (MQ=255)                                                gCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGc                                                                                                  <  7:18019/148‑1 (MQ=255)                                                      ctacAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAAGGTTCAGTTCCTGGGCAGCCTGGCa                                                                                            <  2:321261/148‑1 (MQ=255)                                                         cAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGAc                                                                                         >  7:339388/1‑148 (MQ=255)                                                         cAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGAAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGAc                                                                                         >  4:307646/1‑148 (MQ=255)                                                           aGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGTCTGGCAGACGAt                                                                                      <  2:160148/149‑1 (MQ=255)                                                           aGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGAt                                                                                      <  5:258961/149‑1 (MQ=255)                                                           aGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGAt                                                                                      <  5:114770/149‑1 (MQ=255)                                                               gCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGa                                                                                    >  5:118718/1‑147 (MQ=255)                                                                       aCCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAgcggc                                                                           <  6:118718/148‑1 (MQ=255)                                                                                  gCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCt                                                               >  1:144237/1‑149 (MQ=255)                                                                                        tCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTa                                                         <  3:289739/149‑1 (MQ=255)                                                                                        tCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTa                                                         >  3:267665/1‑149 (MQ=255)                                                                                          tATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACag                                                                                 >  8:4939/1‑123 (MQ=255)                                                                                          tATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACag                                                                                 <  7:4939/123‑1 (MQ=255)                                                                                            tCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAgcggcg                                                                          >  3:98248/1‑128 (MQ=255)                                                                                            tCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAgcggcg                                                                          <  4:98248/128‑1 (MQ=255)                                                                                            tCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTAt                                                        <  8:339388/146‑1 (MQ=255)                                                                                             cAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCt                                                                                                         <  4:259259/96‑1 (MQ=255)                                                                                             cAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCt                                                                                                         >  3:259259/1‑96 (MQ=255)                                                                                                 cGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGa                                                                                                                        >  5:237852/1‑77 (MQ=255)                                                                                                 cGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGa                                                                                                                        <  6:237852/77‑1 (MQ=255)                                                                                                        cGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTcca                                         >  5:8663/1‑149 (MQ=255)                                                                                                          gTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTccatc                                       <  3:363220/149‑1 (MQ=255)                                                                                                                atcatcGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGtt                                                          <  2:351635/124‑1 (MQ=255)                                                                                                                atcatcGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGtt                                                          >  1:351635/1‑124 (MQ=255)                                                                                                                     cggcggTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGtt                            >  3:264107/1‑149 (MQ=255)                                                                                                                       gcggTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTcc                          <  6:8663/149‑1 (MQ=255)                                                                                                                               ttCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGtt                  >  5:175245/1‑149 (MQ=255)                                                                                                                               ttCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGtt                  >  6:138905/1‑149 (MQ=255)                                                                                                                                 ccTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTc                 <  4:267665/148‑1 (MQ=255)                                                                                                                                     gAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCCGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGc              <  1:332576/147‑1 (MQ=255)                                                                                                                                     gAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCAt            >  1:238942/1‑149 (MQ=255)                                                                                                                                      aaGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTgg                                                                                              <  3:5131/66‑1 (MQ=255)                                                                                                                                      aaGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTgg                                                                                              >  4:5131/1‑66 (MQ=255)                                                                                                                                        cctgaGCTC‑AGCAGCCTGCTGCTTGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTtc         >  2:11028/5‑149 (MQ=255)                                                                                                                                        cctgaGCTC‑AGCAGCCTGCTGCTTGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTtc         >  8:112333/5‑149 (MQ=255)                                                                                                                                         ctgaGCTC‑AGCAGCCTGCTGCTTGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTtct        >  3:190148/4‑149 (MQ=255)                                                                                                                                           cAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTctct      <  5:138905/149‑1 (MQ=255)                                                                                                                                           cAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTctct      <  2:144237/149‑1 (MQ=255)                                                                                                                                            aGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTctg     <  7:289547/149‑1 (MQ=255)                                                                                                                                               tCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCAttt          >  6:75399/1‑141 (MQ=255)                                                                                                                                               tCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCAttt          <  5:75399/141‑1 (MQ=255)                                                                                                                                                cAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGCCGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTctgctg  >  2:181247/1‑148 (MQ=255)                                                                                                                                                cAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTctgctg  >  6:202434/1‑148 (MQ=255)                                                                                                                                                 aaGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTctgc    <  2:27020/145‑1 (MQ=255)                                                                                                                                                 aaGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTctgc    >  1:27020/1‑145 (MQ=255)                                                                                                                                                 |                                                                                                                                                    TGGCGCGCACGCATGAGCGAGCGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTCTGCTG  >  NC_002947/2025562‑2025852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑