Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,577,151	Δ1 bp	6.2%	coding (1244/1776 nt)	PP_2259 ←	sigma‑54 dependent transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,577,151	0	A	.	6.2%	206.3 / 6.9	65	coding (1244/1776 nt)	PP_2259	sigma‑54 dependent transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (30/31);  new base . (2/2);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

GGCCGGCGATGCGGTAGTACAGGTCCTGGCGAAAGCGCCCGGCGCGCACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTGTGGTGGGCGCCGGTGAACGCGCCTTTGTCATAGCCGAACAGTTC  >  NC_002947/2577005‑2577296                                                                                                                                                    |                                                                                                                                                  ggCCGGCGATGCGGTAGTACTGGTCCTGGCGAAAGCGCCCGGCGCGCACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTAcg                                                                                                                                                 >  6:10876/1‑149 (MQ=255)  gCCGGCGATGCGGTAGTACAGGTCCTGGCGAAAGCGCCCGGCGCGCACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTAcgc                                                                                                                                                >  1:357425/1‑149 (MQ=255)     ggCGATGCGGTAGTACAGGTCCTGGCGAAAGCGCCCGGCGCGCACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTAcgcg                                                                                                                                               >  6:374273/1‑147 (MQ=255)     ggCGATGCGGTAGTACAGGTCCTGGCGAAAGCGCCCGGCGCGCACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCgca                                                                                                                                             <  4:342936/149‑1 (MQ=255)          tGCGGTAGTACAGGTCCTGGCGAAAGCGCCCGGCGCGCACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAgg                                                                                                                                        >  4:360405/1‑149 (MQ=255)            cGGTAGTACAGGTCCTGGCGAAAGCGCCCGGCGCGCACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCg                                                                                                                                      <  3:111109/149‑1 (MQ=255)             ggTAGTACAGGTCCTGGCGAAAGCGCCCGGCGCGCACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCgg                                                                                                                                     <  5:374273/149‑1 (MQ=255)              gtagtaCAGGTCCTGGCGAAAGCGCCCGGCGCGCACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCggg                                                                                                                                    >  8:49347/1‑149 (MQ=255)                    cAGGTCCTGGCGAAAGCGCCCGGCGCGCACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCAAGCGGCTGGATGCTGCGCG‑ACTGCACTACGCGCAGCAGGCGGGCCtggg                                                                                                                              >  2:323012/1‑149 (MQ=255)                        tCCTGGCGAAAGCGCCCGGCGCGCACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCtgggtggg                                                                                                                          <  2:287106/149‑1 (MQ=255)                        tCCTGGCGAAAGCGCCCGGCGCGCACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCtgggtggg                                                                                                                          <  7:49347/149‑1 (MQ=255)                           tGGCGAAAGCGCCCGGCGCGCACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAg                                                                                                                       >  5:361169/1‑149 (MQ=255)                           tGGCGAAAGCGCCCGGCGCGCACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAg                                                                                                                       >  2:24576/1‑149 (MQ=255)                                     gcCCGGCGCGCACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCg                                                                                                             >  7:185139/1‑149 (MQ=255)                                         ggCGCGCACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCaa                                                                                                         <  5:60271/149‑1 (MQ=255)                                            gcgcACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAAtct                                                                                                      >  1:285314/1‑149 (MQ=255)                                            gcgcACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAAtct                                                                                                      >  3:98785/1‑149 (MQ=255)                                                        ggCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTg                                                                                                                     <  7:339898/122‑1 (MQ=255)                                                        ggCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTg                                                                                                                     >  8:339898/1‑122 (MQ=255)                                                         gCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATc                                                                                                  <  3:47087/140‑1 (MQ=255)                                                         gCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATc                                                                                                  >  4:47087/1‑140 (MQ=255)                                                         gCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAg                                                                                         <  2:357425/149‑1 (MQ=255)                                                          ccAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAgg                                                                                        <  3:360405/149‑1 (MQ=255)                                                          ccAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAgg                                                                                        <  2:105251/149‑1 (MQ=255)                                                              gTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGc                                                                                    >  6:62708/1‑149 (MQ=255)                                                                    aTTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCCGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGGGGGCAGTGGCATGTCGCCAATCTCATCCAGACTCAGGATGCCGTggg                                                                              >  8:100159/1‑148 (MQ=255)                                                                    aTTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGt                                                                              >  5:19351/1‑149 (MQ=255)                                                                      tgggcGCCGACACGACGCGGATATCCCCCGCCACCGGTTCGCCCCTGCCCAGCGGCTGGATGGTGGGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTc                                                                             <  1:323012/144‑1 (MQ=255)                                                                       gCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCgg                                                                           >  2:160130/1‑149 (MQ=255)                                                                           gCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGcc                                                                         <  1:160130/147‑1 (MQ=255)                                                                             cGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGc                                                                                                                   >  4:44270/1‑103 (MQ=255)                                                                             cGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGc                                                                                                                   <  3:44270/103‑1 (MQ=255)                                                                             cGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGc                                                                     <  5:62708/149‑1 (MQ=255)                                                                             cGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGc                                                                     >  4:254796/1‑149 (MQ=255)                                                                               acacCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCgg                                                                   <  5:10876/149‑1 (MQ=255)                                                                                 accacGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGAt                                                                 <  5:59572/149‑1 (MQ=255)                                                                                    acGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAg                                                              >  7:118947/1‑149 (MQ=255)                                                                                     cgcgGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGt                                                             <  6:361169/149‑1 (MQ=255)                                                                                      gcgGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCgg                                                                           >  1:182766/1‑134 (MQ=255)                                                                                         gATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCg                                                         <  8:185139/149‑1 (MQ=255)                                                                                              ccACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTg                                                    >  4:59644/1‑149 (MQ=255)                                                                                                accggcaccggTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGcc                                                  >  5:229284/1‑149 (MQ=255)                                                                                                accggcaccggTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGcc                                                  >  7:283145/1‑149 (MQ=255)                                                                                                    gcaccggTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTtg                                              >  7:32196/1‑149 (MQ=255)                                                                                                        cggTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTgtggt                                          <  3:254796/149‑1 (MQ=255)                                                                                                         ggTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTgtggtg                                         <  6:229284/149‑1 (MQ=255)                                                                                                          gTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTTGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTGtggtgg                                        >  4:338845/1‑149 (MQ=255)                                                                                                              ggctctgcgctgcgGCTGGATGCGGCGCG‑CCTGCTGTACGGGCAGCAGGCGGGGCTGGGTGGGCAGTGGCTTGTCGCCATTCTCATCCAGAAAGAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTGTGGTGGgcgc                                    <  2:285314/145‑1 (MQ=255)                                                                                                               ccACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCaa                                                                                                         >  3:337399/1‑79 (MQ=255)                                                                                                               ccACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCaa                                                                                                         <  4:337399/79‑1 (MQ=255)                                                                                                                      ccAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTGTGGTGGGCGCCGGTGAAc                            <  1:69419/149‑1 (MQ=255)                                                                                                                      ccAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTGTGGTGGGCGCCGGTGAAc                            >  2:69419/1‑149 (MQ=255)                                                                                                                          cGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTGTGGTGGGCGCCGGTGAACgcgc                        <  6:19351/149‑1 (MQ=255)                                                                                                                                gATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTGTGGTGGGCGCCGGTGAACGCGCCTTTGt                  >  8:224543/1‑149 (MQ=255)                                                                                                                                     tgcGCGTACTGCAGT‑CGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTGTGGTGGGCGCCGGTGAACGCGCCTTTGTCATAg             >  4:375356/1‑149 (MQ=255)                                                                                                                                     ccgtagaaCTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTGTGGTGGGCGCCGGTGAACGCGCCTTTGTCATa              <  6:146413/142‑1 (MQ=255)                                                                                                                                      gcgcgtaCTGCAGT‑CGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTGTGGTGGGCGCCGGTGAAc                            >  2:212127/7‑133 (MQ=255)                                                                                                                                      gcgcgtaCTGCAGT‑CGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGCGTTGCCCTTGTGGTGGGCGCCGGTGAAc                            <  1:212127/127‑1 (MQ=255)                                                                                                                                       cgcg‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGGGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTGTGGTGGGCGCCGGTGAACGCGCCTTTGTCATAGcc           <  4:123017/149‑1 (MQ=255)                                                                                                                                          g‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTGTGGTGGGCGCCGGTGAACGCGCCTTTGTCATAGCCGaa        <  3:59644/149‑1 (MQ=255)                                                                                                                                          g‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTGTGGTGGGCGCCGGTGAACGCGCCTTTGTCATAGCCGaa        <  8:283145/149‑1 (MQ=255)                                                                                                                                            aCTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTGTGGTGGGCGCCGGTGAACGCGCCTTTGTCATAGc            >  1:225635/1‑144 (MQ=255)                                                                                                                                            aCTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTGTGGTGGGCGCCGGTGAACGCGCCTTTGTCATAGc            <  2:225635/144‑1 (MQ=255)                                                                                                                                             cTGCAGT‑CGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTGTGGTGGGCGCCGGTGAACGCGCCTTTGTCATAGCCGAACa      <  3:375356/148‑1 (MQ=255)                                                                                                                                                 aGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTGTGGTGGGCGCCGGTGAACGCGCCTTTGTCATAGCCGAACAGTTc  <  8:118947/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GGCCGGCGATGCGGTAGTACAGGTCCTGGCGAAAGCGCCCGGCGCGCACTTCGTCGGCCAGGTTGCGATTGCTCGCCGACACCACGCGGATATCCACCGGCACCGGTTCGCCACTGCCCAGCGGCTGGATGCTGCGCG‑ACTGCAGTACGCGCAGCAGGCGGGCCTGGGTGGGCAGTGGCATGTCGCCAATCTCATCCAGAAACAGGATGCCGTGGTCGGCCTTGCGGATCAGTCCCGGGTTGCCCTTGTGGTGGGCGCCGGTGAACGCGCCTTTGTCATAGCCGAACAGTTC  >  NC_002947/2577005‑2577296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑