| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 278,742:1 | +A | 100% | intergenic (‑237/+77) | PP_0224 ← / ← sctC | DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 278,742 | 1 | . | A | 94.4% | 109.3 / 0.3 | 36 | intergenic (‑237/+77) | PP_0224/sctC | DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base A (14/20); total (15/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGT‑AA‑CCCGCTCCTACACACA‑TGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCT > NC_002947/278596‑278885 | tGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGT‑a > 2:117888/1‑148 (MQ=255) atcttGATCGCGACAGAAGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTc < 7:349928/145‑1 (MQ=255) cTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTa < 2:259044/149‑1 (MQ=255) gATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTc > 8:349928/1‑144 (MQ=255) aTCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATATAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTacac < 2:21202/149‑1 (MQ=255) gcgACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTAcacaca > 4:196905/1‑149 (MQ=255) cgACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACACACA‑t < 5:159416/149‑1 (MQ=255) cgACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACACACA‑t < 2:131185/149‑1 (MQ=255) aCGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACACACA‑TGcc > 4:58591/1‑146 (MQ=255) aCGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACACACA‑TGcc < 3:58591/146‑1 (MQ=255) cGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACACACA‑TGCCTCaa < 6:168715/149‑1 (MQ=255) gtccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACACACA‑TGCCTCAAGATCtgt < 8:229311/147‑1 (MQ=255) ggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACACACA‑TGCCTCAAGATCTGTGCACTg < 4:273363/149‑1 (MQ=255) gtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTaca < 5:267901/123‑1 (MQ=255) gtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTaca > 6:267901/1‑123 (MQ=255) tggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACACACA‑TGCCTCAAGATCTGTGCACTGTa < 6:222496/149‑1 (MQ=255) ccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑ACCGCTACTACAAACA‑TGCCTCAAGATATGTGCACTGTAGGAg > 1:373776/1‑149 (MQ=255) gtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCt > 8:288961/1‑112 (MQ=255) gtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCt < 7:288961/112‑1 (MQ=255) tataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACACACA‑TGCCTCAAGATCTGTGCACTGTAGGAGCGGGttt < 6:23948/148‑1 (MQ=255) cccTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACACACA‑TGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCcg < 8:294380/149‑1 (MQ=255) tAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACACACA‑TGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCcgcg > 2:103528/1‑148 (MQ=255) aTGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCCTCCCTTCGCGGGTAAA‑CCCGCTCCTACACACA‑TGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCcgc < 1:117888/148‑1 (MQ=255) tGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑ACCGCTCCTACACACA‑TGCCGAAAGATATGTGCACTGTAGGAGCGGGTTTACCCGCGAAAg > 2:324833/1‑149 (MQ=255) gATTTATAAAATTTTTATGAACTAACTTTTTGGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑AT‑‑CTTCGAGGGTAAA‑CCCGCTCCTACACACA‑TGCATCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAg > 1:40047/1‑149 (MQ=255) ataAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑AACGCTACTTAACAAA‑TTCGGCAATATATGTGGACTGTATGAGGGGGTTTTCCCGCGAAAAGGCCgg > 5:262656/1‑149 (MQ=255) tttATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACACACATTTCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAgg < 1:103528/148‑1 (MQ=39) taactTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACAAACA‑TGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAg > 5:151859/1‑149 (MQ=255) ttt‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACACACA‑TGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGa < 2:41481/142‑1 (MQ=255) ttt‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACACACA‑TGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGa > 1:41481/1‑142 (MQ=255) ttt‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGCGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACACACA‑TGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCg < 1:102076/147‑1 (MQ=255) gAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGT‑AATCCCGCTCCTACACACA‑TGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTg > 5:89983/1‑149 (MQ=255) aaGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACACACA‑TGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTgc < 6:151859/149‑1 (MQ=255) gTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACACACA‑TGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGc < 7:284905/140‑1 (MQ=255) gTGCCGGG‑CT‑‑CTTCGCGGGTAAA‑CCCGCTCCTACACACA‑TGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGc > 8:284905/1‑140 (MQ=255) cgcgGGTAAA‑CCCGCTCCTACACACA‑TGCCTCAAGCTCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGgcg > 3:354988/1‑149 (MQ=255) cgGGT‑AATCCCGCTCCTACACACA‑TGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCt < 6:89983/149‑1 (MQ=255) | TGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTT‑GGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGG‑CT‑‑CTTCGCGGGT‑AA‑CCCGCTCCTACACACA‑TGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCT > NC_002947/278596‑278885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |