| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 336,124:1 | +T | 100% | coding (209/237 nt) | PP_0278 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 336,124 | 1 | . | T | 98.2% | 181.3 / ‑3.7 | 55 | coding (209/237 nt) | PP_0278 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (26/28); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑A‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCG‑TCGCGAATGGGCTGCAAAGCAGCCCCGG > NC_002947/335980‑336253 | ccccATCCCTCAATCTCCTACGCGTAGAGCCCGGCGGCCCCGTCGCCGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCGGGGGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑A‑ttac < 8:16999/148‑4 (MQ=255)aCCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑A‑tta < 3:168453/148‑3 (MQ=255) cccATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑A‑ttacc > 6:13723/1‑145 (MQ=255) ccATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACTATAAATTCCTGGGGGCAGCCG‑A‑ttacct > 6:109563/1‑144 (MQ=255) cATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCtg > 4:375274/1‑149 (MQ=255) tCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCtgtg < 6:18076/149‑1 (MQ=255) ccTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATTGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGc < 6:302599/148‑1 (MQ=255) tCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGa > 2:41559/1‑148 (MQ=255) aaTCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACAt > 4:51314/1‑149 (MQ=255) ccTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGcc > 3:297460/1‑148 (MQ=255) aCGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATg < 4:23903/142‑1 (MQ=255) aCGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATg > 3:23903/1‑142 (MQ=255) cgcgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAgg < 4:297460/148‑1 (MQ=255) gagCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCtga < 1:41559/148‑1 (MQ=255) gCCCGGCGTCCCCGTCGCCGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCtgatg > 8:88120/1‑148 (MQ=255) ccGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAAc < 3:375274/149‑1 (MQ=255) ggCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGa > 2:355863/1‑149 (MQ=255) ggCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGa < 3:51314/149‑1 (MQ=255) aCGCGGCCTACGATCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGcc < 5:13723/149‑1 (MQ=255) gAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGgc < 1:231523/149‑1 (MQ=255) gTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCAtt < 2:216757/148‑1 (MQ=255) gTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCAtt > 1:216757/1‑148 (MQ=255) gTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCAt > 4:32197/1‑147 (MQ=255) tctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACTATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAg < 5:109563/149‑1 (MQ=255) ggtCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGGCGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAgg < 4:62522/129‑1 (MQ=255) ggtCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGGCGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAgg > 3:62522/3‑131 (MQ=255) aTGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGAcgcg < 5:184500/149‑1 (MQ=255) gggCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGt > 5:257703/1‑149 (MQ=255) gCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCa < 6:179141/149‑1 (MQ=255) cTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAt < 7:88120/149‑1 (MQ=255) tCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCgg < 1:156563/140‑1 (MQ=255) tCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCgg > 2:156563/1‑140 (MQ=255) aGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGa < 5:357061/149‑1 (MQ=255) gCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAg > 2:34451/1‑109 (MQ=255) gCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAg > 1:218279/1‑149 (MQ=255) gCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAg > 3:233619/1‑149 (MQ=255) gCACCTGGGGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAACGCGCTGATGAACGATATCGAGATCGCCAAg < 1:34451/109‑1 (MQ=255) cTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGc < 3:32197/149‑1 (MQ=255) cTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGc > 1:253318/1‑149 (MQ=255) gcgcGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGc > 8:75496/1‑149 (MQ=255) cgcgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCg < 1:355863/149‑1 (MQ=255) aGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCG‑Tcgc > 3:151325/1‑149 (MQ=255) aGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCG‑Tcgc < 7:204647/149‑1 (MQ=255) atatGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCG‑TCGCGAATgg < 7:75496/149‑1 (MQ=255) gaggaCGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCG‑TCGCGAATGGGCTg < 1:151921/148‑1 (MQ=255) tcgacCATAAATTCCTGGGGGGAGGCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGGCTTGCG‑TCGCGAATGGGCTGctttg < 2:253318/148‑5 (MQ=255) ccATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGGCTTGCG‑TGGCGGAAGGGCGGCCAagcggc > 2:102114/1‑149 (MQ=255) ccATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCG‑TCGCGAATGGGATGCAAagcagc > 6:366504/1‑149 (MQ=255) aTAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGc > 7:72662/1‑91 (MQ=255) aTAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGc < 8:72662/91‑1 (MQ=255) aTAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCGATGGGTTCGGGAATGGGTTGCaagaaaccc > 1:369603/1‑142 (MQ=255) aTAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCG‑Tcgc > 7:123027/1‑128 (MQ=255) aTAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCG‑Tcgc > 7:128435/1‑128 (MQ=255) aTAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCG‑Tcgc < 8:123027/128‑1 (MQ=255) aTAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCG‑Tcgc < 8:128435/128‑1 (MQ=255) aaaTTCCTGGGGGCAGCCGAA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCG‑TCGCGAATGGGCTGc < 3:258075/137‑1 (MQ=255) aaaTTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCG‑TCGCGAATGGGCTGc > 4:258075/1‑137 (MQ=255) aaTTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCTTTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCG‑TCGCGAATGGGCTGCTTTGCAGcccatt < 4:233619/149‑4 (MQ=39) aaTTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCG‑TCGCGAATGGGCTGCAAAGCAGCCCCgg > 6:330037/1‑149 (MQ=255) | ACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑A‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCG‑TCGCGAATGGGCTGCAAAGCAGCCCCGG > NC_002947/335980‑336253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |