| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,586,033:1 | +G | 100% | intergenic (+116/+99) | PP_4061 → / ← PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,586,033 | 1 | . | G | 92.3% | 33.3 / ‑2.6 | 13 | intergenic (+116/+99) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (2/10); total (2/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑G‑GTAAACCCGCTCCTACCAGGGA‑CGT‑GCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTAC > NC_002947/4585930‑4586103 | gCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGT‑GCCGGCCTTGAATgcgg < 1:82555/149‑1 (MQ=39) aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGTCGCCGGCCTTGAATGCGGCGCTGcccc < 1:3512/148‑1 (MQ=38) aCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGT‑GCCGGCCTTGAATGCGGCGCTGCCCCTgg < 4:127632/149‑1 (MQ=39) aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGT‑GCCGGCCTTGAATGCGGCGCTGCCCCTGGTa < 3:235960/149‑1 (MQ=39) gCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGT‑GCCGGCCTTGAATGCGGCGCTGCCCCTGGTAg < 7:155275/149‑1 (MQ=39) gCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGT‑GCCGGCCTTGAATGCGGCGCTGCCCCTGGTAg > 8:155275/1‑149 (MQ=39) cgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGT‑GCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCggg < 4:121726/147‑1 (MQ=38) cgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGT‑GCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCggg < 7:139934/147‑1 (MQ=38) cctgctCCATTGAAAGGCTGACGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑GCGTAAACCCGCTCCTACCAGCGACCGT‑GCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGttt < 4:99872/148‑1 (MQ=38) ctgctCCATTGAAAGGTTGAGGATGTCCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGT‑GCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTa < 5:66604/149‑1 (MQ=38) ctCCATTGAAAGGTTGAGGATTTCCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGT‑GCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTAc < 3:274528/147‑1 (MQ=37) ggTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGT‑GCCGGCCTTGAATgcg > 1:271275/1‑93 (MQ=25) ggTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGT‑GCCGGCCTTGAATgcg < 2:271275/93‑1 (MQ=25) | GCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑G‑GTAAACCCGCTCCTACCAGGGA‑CGT‑GCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTAC > NC_002947/4585930‑4586103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |