Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,682,024:1 +A 100% coding (314/1431 nt) mltD → membrane‑bound lytic murein transglycosylase D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,682,0241.A97.8% 147.8 / ‑3.6 45coding (314/1431 nt)mltDmembrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base A (22/22);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTCCCCGCTGCTGGTCAAGCCGGCCGAACAGGCGCCGCAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TT‑CATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTCACGCGCCAGC  >  NC_002947/4681880‑4682166
                                                                                                                                                   |                                                                                                                                              
cTTCCCCGCTGCTGGTCAAGCCGGCCGAACAGGCGCCGCAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑ttacat                                                                                                                                             >  3:216408/1‑145 (MQ=255)
              gTCAAGCCGGCCGAACAGGCGCCGCAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCggg                                                                                                                                >  2:334100/1‑148 (MQ=255)
                 aaGCCGGCCGAACAGGCGCCGCAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAGCCGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGaa                                                                                                                             <  1:89167/149‑1 (MQ=255)
                   gccggccgAACAGGCGCCGCAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGt                                                                                                                          <  4:373401/149‑1 (MQ=255)
                         cgAACAGGCGCCGCAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGc                                                                                                                    >  5:147171/1‑149 (MQ=255)
                              aGGCGCCGCAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTTGTTCGCCAGCAACCCGACC‑TTACATCGaa                                                                                                                                         <  6:108487/123‑1 (MQ=255)
                              aGGCGCCGCAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGaa                                                                                                                                         >  5:108487/1‑123 (MQ=255)
                              aGGCGCCGCAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTa                                                                                                               <  3:80923/149‑1 (MQ=255)
                                     gcAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCAc                                                                                                        >  1:296087/1‑149 (MQ=255)
                                      cAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACt                                                                                                       <  4:216408/149‑1 (MQ=255)
                                         gATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACa                                                                                                    <  3:125362/149‑1 (MQ=255)
                                             tCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACAtcgt                                                                                                >  3:221123/1‑149 (MQ=255)
                                              cTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACAtcgtc                                                                                               >  3:339113/1‑149 (MQ=255)
                                               tGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCCACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCCGCAACCCGCCC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCACTACCTGCACTACAtcgtc                                                                                               >  2:5189/1‑148 (MQ=255)
                                                  gAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACTTGCACTACATCGTCGAgc                                                                                           <  5:354546/149‑1 (MQ=255)
                                                         tGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTga                                                                                    <  6:147171/149‑1 (MQ=255)
                                                             ccAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTgaagaa                                                                                >  7:158120/1‑149 (MQ=255)
                                                                       gCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACCGTA‑CATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTCAAGAACGCGACATGc                                                                      >  3:106958/1‑149 (MQ=255)
                                                                       gCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGc                                                                      <  5:96756/149‑1 (MQ=255)
                                                                        cccTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCCGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGcc                                                                     >  3:292471/1‑149 (MQ=255)
                                                                           tGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCt                                                                  >  4:221479/1‑149 (MQ=255)
                                                                             cAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCt                                                                  <  4:339113/147‑1 (MQ=255)
                                                                                      aTCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGcc                                                                                        <  3:327836/116‑1 (MQ=255)
                                                                                      aTCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGcc                                                                                        >  4:327836/1‑116 (MQ=255)
                                                                                         gATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCctg                                                     <  1:334100/148‑1 (MQ=255)
                                                                                               aaCCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTTGCCCTGCTgcca                                               <  6:354483/148‑1 (MQ=255)
                                                                                                aCCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGCACTGGCCCTGCTgccagc                                             >  6:280132/1‑149 (MQ=255)
                                                                                                 cccGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTgccagc                                             >  6:377443/1‑148 (MQ=255)
                                                                                                    gcgcATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATc                                         >  5:65428/1‑149 (MQ=255)
                                                                                                         tCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAg                                    <  8:158120/149‑1 (MQ=255)
                                                                                                             gcagcag‑cGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGcc                                <  2:296087/149‑1 (MQ=255)
                                                                                                               agcag‑cGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTa                              >  1:220859/1‑149 (MQ=255)
                                                                                                                 cag‑cGCTTGTGGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTAc                             >  4:336281/1‑148 (MQ=255)
                                                                                                                          gtgGTTCGCCAGCAACCCGACC‑TTACTTCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCGCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATg                    >  6:215468/1‑149 (MQ=255)
                                                                                                                           tgGTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATg                    <  2:220859/148‑1 (MQ=255)
                                                                                                                            gggtCGCCAGCAACCCGACC‑TTACATCGCTAGCGCAGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGc                  <  4:221123/146‑1 (MQ=255)
                                                                                                                            ggTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAATGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGc                  >  5:58279/1‑149 (MQ=255)
                                                                                                                             gTTCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGcc                 >  4:108189/1‑149 (MQ=255)
                                                                                                                             gTTCGCCAGCAACCCGACA‑TTACATCGAAAGCGCCGGGCAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGc                  >  8:316296/1‑148 (MQ=255)
                                                                                                                               tCGCCAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTa               >  1:361857/1‑149 (MQ=255)
                                                                                                                                  ccAGCTACCCGACC‑TTACATCGACAGCGCGGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTc            <  4:106958/149‑1 (MQ=255)
                                                                                                                                   cAGCAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACACACCCATGGCCTACTcc           >  2:243532/1‑148 (MQ=255)
                                                                                                                                      cAACCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTCAcgc        <  6:133069/149‑1 (MQ=255)
                                                                                                                                        aCCCGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTCACgcgc      <  3:336281/149‑1 (MQ=255)
                                                                                                                                           cGACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTCACGCGCCAg   <  1:149589/149‑1 (MQ=255)
                                                                                                                                            gACC‑TTACATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTCACGCGCCAgc  <  5:377443/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
CTTCCCCGCTGCTGGTCAAGCCGGCCGAACAGGCGCCGCAGGATGTCTGGGAGCGCATGCGCCAGGGCTTCGCCCTGCAGGACAGTATCGATGTCAACCCGCGCATCGAGCAGCAG‑CGCTTGTGGTTCGCCAGCAACCCGACC‑TT‑CATCGAAAGCGCCGGGGAACGTGGCAGCCTCTACCTGCACTACATCGTCGAGCGCCTTGAAGAACGCGACATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTCACGCGCCAGC  >  NC_002947/4681880‑4682166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑