| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,674,750:1 | +G | 100% | intergenic (+112/+28) | rhlE‑II → / ← yceI | ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,674,750 | 1 | . | G | 97.6% | 283.1 / ‑0.3 | 85 | intergenic (+112/+28) | rhlE‑II/yceI | ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (37/46); total (38/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.00e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑C‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCA > NC_002947/5674603‑5674892 | ggTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑g‑c‑g < 2:372040/149‑1 (MQ=255)ggTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑g‑c‑g < 4:102749/149‑1 (MQ=255)ggTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑g‑c‑g > 4:58289/1‑149 (MQ=255)ggTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑g‑c‑g < 1:67557/149‑1 (MQ=255) tGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑g‑c‑ggc > 8:89042/1‑147 (MQ=255) gcCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑g‑c‑ggccccct < 5:276797/149‑8 (MQ=255) gcCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑g‑c‑ggccccct < 8:325998/149‑8 (MQ=255) gcgcTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGtt > 3:100085/1‑149 (MQ=255) ctgctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTc < 3:58289/149‑1 (MQ=255) ctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCa < 8:157778/147‑1 (MQ=255) ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGGGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTgg > 1:309864/1‑147 (MQ=255) ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTgg > 3:261706/1‑147 (MQ=255) ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTgg > 3:35847/1‑147 (MQ=255) cAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACTACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGAGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAg > 1:284238/1‑148 (MQ=255) aaGGGCTGATTGTTGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGaaa < 2:331912/149‑1 (MQ=37) gCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTAc < 1:4107/149‑1 (MQ=255) tGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACt > 4:266387/1‑148 (MQ=255) gATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCAGCAGAGAATTTCTTGATCTCTACAGCAGCGGCCTTGTGTCGTGATAGGGCAGCAAA‑G‑CGGCCCCCACGGTTTTCAGCTGGCAGAAAATACtt > 2:278241/1‑148 (MQ=37) aGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGc < 5:219183/149‑1 (MQ=255) gAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCt > 4:363662/1‑149 (MQ=255) aaGTACCGAGGGGGCTGCTTCTCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGG‑G‑GCCCCCTCGGTTTTCAGCTGGCAGaaa < 5:61921/137‑1 (MQ=37) aaGTACCGAGGGGGCTGCTTCGCAGCCCTATAACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCGTGTGTCGTGATACGGCCGCAAA‑G‑CGGCCCCCGCGGTTTAGAGCTGGCAGaaa > 6:61921/1‑137 (MQ=25) aGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAGTAATTTCTGccag < 6:85921/149‑4 (MQ=25) gTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGATTGTCTTGATCTCTACAGGAGCGGCCTGGTGTCGAGATAGGGCCGCAGA‑GCC‑GCCCCCTCGGATTTACGCTGGCAGAAATGACTTCTGCTTca > 5:229278/1‑149 (MQ=25) tACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTca > 7:247693/1‑148 (MQ=255) ccGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTcac < 1:113876/147‑1 (MQ=255) ctgctTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGacac < 3:363662/149‑1 (MQ=255) ctTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTg < 2:309864/148‑1 (MQ=255) ctTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGa > 3:252288/1‑149 (MQ=255) ctTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGa > 5:235094/1‑149 (MQ=255) ttCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTcac > 3:213667/1‑134 (MQ=255) ttCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTcac < 4:213667/134‑1 (MQ=255) ttCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGa > 1:190393/1‑148 (MQ=255) cAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGTCCTTGTTTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtcc < 5:202782/149‑1 (MQ=38) gCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag < 3:266387/149‑1 (MQ=255) tATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtc > 6:291102/1‑148 (MQ=255) aCGAC‑TCATGGCCGCTACTGCAGAGAATTTCTTGATCTCTAGAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg < 1:278241/148‑1 (MQ=38) aCGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg < 8:247693/148‑1 (MQ=255) aCGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg < 3:338596/148‑1 (MQ=255) c‑acaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGACGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTg > 2:227592/1‑149 (MQ=255) aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag > 4:48612/1‑134 (MQ=255) aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag < 3:48612/134‑1 (MQ=255) aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCg > 3:59951/1‑148 (MQ=255) tgCCGCTCCTGCAGAGAATATCTTGATCTCTACCTGATCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAATTTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAg < 6:32194/148‑1 (MQ=255) gCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGTCCTTGTTTAGTTATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGt < 7:162644/149‑1 (MQ=255) cTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCa < 4:35847/149‑1 (MQ=255) cTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCAccc > 6:102955/1‑149 (MQ=255) cTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCAcc < 4:59951/148‑1 (MQ=255) tGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCg > 3:154902/1‑149 (MQ=255) cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCgg < 7:61552/148‑1 (MQ=255) gaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCtt < 6:235094/149‑1 (MQ=255) gaATTTCATGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTCCTTCACTCCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCt < 6:229278/148‑1 (MQ=38) tCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGAt < 2:236981/148‑1 (MQ=255) ttGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGcc > 5:358449/1‑149 (MQ=255) aTCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa < 5:201819/149‑1 (MQ=255) tctcTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa < 6:358449/148‑1 (MQ=255) tctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCttt < 3:253696/137‑1 (MQ=255) tctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCttt > 4:253696/1‑137 (MQ=255) ctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTgg > 4:30407/1‑61 (MQ=18) ctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTgg < 3:30407/61‑1 (MQ=18) ttcAGGAGCGGCCTTGTGTCGTGATAGGGCCTCAAA‑G‑CGGCCCACTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAg < 4:261706/143‑1 (MQ=255) tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa < 5:102955/144‑1 (MQ=255) tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa < 4:100085/144‑1 (MQ=255) tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCAAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGag > 6:201819/1‑143 (MQ=255) aGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttca < 5:291102/149‑1 (MQ=255) aGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt > 2:30593/1‑147 (MQ=255) gAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg < 4:252288/149‑1 (MQ=255) gAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttca < 1:109899/147‑1 (MQ=255) gAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttca > 2:109899/1‑147 (MQ=255) gCCTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggttcag > 3:229631/1‑149 (MQ=255) cTTGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCg < 8:62202/149‑1 (MQ=255) ttGTGTCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgt > 3:233069/1‑149 (MQ=255) ccttgtgTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtg < 1:324319/143‑1 (MQ=255) tCGTGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtgg > 5:236545/1‑149 (MQ=255) gTGATAGGGCCGCAAA‑G‑CGGCCCCCGCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg < 6:390879/149‑1 (MQ=255) tGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtg > 2:324319/1‑142 (MQ=255) tGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGtggtggtgg > 2:225793/1‑149 (MQ=255) tGATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGtggtggtgg > 2:109267/1‑149 (MQ=255) gATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg > 1:249013/1‑87 (MQ=255) gATAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg < 2:249013/87‑1 (MQ=255) aTAGGGCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCNGAAGTCGTTCAGGTTCAGCGTGGTGGTGGc < 1:30593/148‑1 (MQ=255) gggCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt < 1:227592/148‑1 (MQ=255) gggCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt < 1:225793/148‑1 (MQ=255) ggCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg > 6:58604/1‑148 (MQ=255) gCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa < 5:19521/149‑1 (MQ=255) gCCGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa < 4:233069/149‑1 (MQ=255) cctcAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAg < 1:24257/146‑1 (MQ=255) ccGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAg > 2:24257/1‑149 (MQ=255) cGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGc > 5:309958/1‑149 (MQ=255) cGCAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGc > 5:309957/1‑149 (MQ=255) cAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCa < 7:217299/149‑1 (MQ=255) cAAA‑G‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCa < 7:89042/149‑1 (MQ=255) | GGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGAC‑ACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑G‑C‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCA > NC_002947/5674603‑5674892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |