| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,126,645:1 | +C | 100% | intergenic (‑99/+50) | tdcG‑II ← / ← gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,126,645 | 1 | . | C | 83.3% | 65.7 / 8.0 | 24 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (4/0); new base C (9/11); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.83e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AAA‑CC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGG > NC_002947/1126504‑1126770 | ggCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AAA‑cccgctc < 4:306778/149‑6 (MQ=255) gatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGCAAGCGGAGGGTAAGGCACCAACCCCATCGCCGG‑TGAAC‑CC‑GCACCCACACagcc > 6:150081/1‑147 (MQ=255) gAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGccc < 3:141137/146‑1 (MQ=255) gTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCGCGGATGCGGGGGGTTAGGCCCCCACGCCATCGCGGGTT‑AAC‑CC‑GCGCCGACACAGAGccccccccccca > 6:94222/1‑147 (MQ=255) gAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCa < 6:106106/141‑1 (MQ=255) gAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCa > 5:106106/1‑141 (MQ=255) tGACCTGGGGCCCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGa < 5:150081/149‑1 (MQ=38) ccAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGTAGGTATAGCCACCATCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCt < 4:366172/148‑1 (MQ=255) aCATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGAc < 5:94222/149‑1 (MQ=255) tGAGCCCCGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcga < 5:142485/140‑1 (MQ=255) gAGCCCTGTAGGCGCGGGTTTAACCGCGAATGCGGAGGGATAGCCACCCACGCATTAGCGGG‑TAAAA‑CC‑GCAGCAACACAGAGCCCCCCACCCCACCCAGGTGGCGGGGGGGGCTATAAGGCGGGCGGATTGCGCTCGCACGACggg > 2:88910/1‑147 (MQ=255) tGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAggg < 5:116218/107‑1 (MQ=255) tAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTGGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGcaca > 1:127478/1‑149 (MQ=255) GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTGGCGGG‑T‑AAA‑CCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGtt > 2:127478‑M1/1‑149 (MQ=255) gTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGc < 3:30592/149‑1 (MQ=255) cccGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCg > 1:148750/1‑149 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc > 7:229711/1‑149 (MQ=255) cgcgAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGcc > 1:210637/1‑149 (MQ=255) gcgAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGcc > 1:333737/1‑148 (MQ=255) ggAGGTATAGCCACCATCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTc > 4:204131/1‑137 (MQ=255) ggAGGTATAGCCACCATCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTc < 3:204131/137‑1 (MQ=255) taGCCACCATCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAc < 7:201757/148‑1 (MQ=255) gCCACCATCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGt < 5:320881/127‑1 (MQ=255) gCCACCATCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGt > 6:320881/1‑127 (MQ=255) ccaccaTCGCATTCGCGGG‑T‑AAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg > 2:142821/1‑149 (MQ=255) | GGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AAA‑CC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGG > NC_002947/1126504‑1126770 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |