Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	185,372:1	+A	5.9%	coding (241/957 nt)	PP_0161 ←	putative transmembrane sensor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	185,372	1	.	A	5.9%	275.4 / 9.5	68	coding (241/957 nt)	PP_0161	putative transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base . (31/33);  new base A (2/2);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.45e-01

GCAGGGTGAAGACGCGTCGCTCGCCCGTGCCGGTGGCA‑TAGTCTGACGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTCGTGGTGGCTGCTGCGCCATTGCGCGCACTGTTGT  >  NC_002947/185231‑185520                                                                                                                                                |                                                                                                                                                     gCAGGGTGAAGACGCGTCGCTCGCCCGTGCCGGTGGCA‑TAGTCTGACGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGgcgc                                                                                                                                               <  8:98782/149‑1 (MQ=255)     ggTGAAGACGCGTCGCTCGCCCGTGCCGGTGGCATTAGTCTGACGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGCCGGCG‑CAGGCgctgc                                                                                                                                            >  1:242684/1‑149 (MQ=255)     ggTGAAGACGCGTCGCTCGCCCGTGCCGGTGGCA‑TAGTCTGACGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTG‑CGCCGTCGACAGGCgctgaa                                                                                                                                           <  7:188325/149‑3 (MQ=255)       tGAAGACGCGTCGCTCGCCCGTGCCGGTGGCA‑TAGTCTGACGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTgctgg                                                                                                                                         >  5:83075/1‑149 (MQ=255)          aGACGCGTCGCTCGCCCGTGCCGGTGGCA‑TAGTCTGACGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTgctggctg                                                                                                                                      <  4:359357/149‑1 (MQ=255)          aGACGCGTCGCTCGCCCGTGCCGGTGGCA‑TAGGCTGACGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTgctggctg                                                                                                                                      <  3:23197/149‑1 (MQ=255)           gACGCGTCGCTCGCCCGTGCCGGTGGCA‑TAGTCTGACGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGctggctgg                                                                                                                                     >  3:353395/1‑149 (MQ=255)                 tcgctcgcCCGTGCCGGTGGCA‑TAGTCTGACGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCa                                                                                                                               <  4:353395/149‑1 (MQ=255)                 tcgctcgcCCGTGCCGGTGGCA‑TAGTCTGACGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCa                                                                                                                               <  5:82750/149‑1 (MQ=255)                 tcgctcacCCGTGCCGGTGGCA‑TAGTCTGACGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCa                                                                                                                               <  3:294058/149‑1 (MQ=255)                  cgctcgcCCGTGCCGGTTTCA‑TAGTCTGACGTCCAGGCAGTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCTTCCCTTCCATGACAACGCCACCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAg                                                                                                                              <  3:268315/149‑1 (MQ=255)                   gctcgcCCGTGCCGGTGGCA‑TAGTCTGACGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAg                                                                                                                              <  2:242684/148‑1 (MQ=255)                         ccGTGCCGGTGGCA‑TAGTCTGACGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTg                                                                                                                        >  3:332972/1‑148 (MQ=255)                              ccGGTGGCA‑TAGTCTGACGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGc                                                                                                                  <  4:109466/149‑1 (MQ=255)                                ggTGGCA‑TAGTCTGACGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCa                                                                                                                <  6:41130/149‑1 (MQ=255)                                 gTGGCA‑TAGTCTGACGTCCAGGCACTCAGAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGc                                                                                                                  >  1:214040/1‑146 (MQ=255)                                 gTGGCA‑TAGTCTGACGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGc                                                                                                                  <  2:214040/146‑1 (MQ=255)                                             tGACGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCgctgc                                                                                                                                            >  2:114519/1‑109 (MQ=255)                                                cGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGa                                                                                                 <  7:249832/149‑1 (MQ=255)                                                         aCTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCa                                                                                        <  4:332972/149‑1 (MQ=255)                                                         aCTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCa                                                                                        <  4:98155/149‑1 (MQ=255)                                                         aCTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCa                                                                                        <  3:35096/149‑1 (MQ=255)                                                           tCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAgg                                                                                      >  7:170046/1‑149 (MQ=255)                                                            cacaGTATCGAGGTCTTTGGCCCGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGt                                                                                     >  2:242602/1‑149 (MQ=255)                                                                     gAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCt                                                                            >  6:187072/1‑149 (MQ=255)                                                                       ggTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCa                                                                                        >  2:204413/1‑135 (MQ=255)                                                                       ggTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCa                                                                                        <  1:204413/135‑1 (MQ=255)                                                                        gTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTAt                                                                         >  6:159986/1‑149 (MQ=255)                                                                           tttGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATg                                                                        >  4:70637/1‑147 (MQ=255)                                                                           tttGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCA‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATg                                                                        >  8:234093/1‑147 (MQ=255)                                                                               gccagccaGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGt                                                                   >  1:187352/1‑148 (MQ=255)                                                                               gccagccaGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGt                                                                   <  4:129927/148‑1 (MQ=255)                                                                                 cagccagcTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGaa                                                                >  7:203646/1‑149 (MQ=255)                                                                                 cagccagcTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGGGAAGGTCCTTATGAAGGTgca                                                                >  8:311370/1‑147 (MQ=255)                                                                                     cagcTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATa                                                                                               >  4:242901/1‑114 (MQ=255)                                                                                     cagcTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATa                                                                                               <  3:242901/114‑1 (MQ=255)                                                                                     cagcTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGaa                                                                      >  1:161683/1‑139 (MQ=255)                                                                                     cagcTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGaa                                                                      <  2:161683/139‑1 (MQ=255)                                                                                       gcTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAAc                                                               <  4:252347/144‑1 (MQ=255)                                                                                       gcTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAAc                                                               >  3:252347/1‑144 (MQ=255)                                                                                         tCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTa                                                                          >  2:80938/1‑131 (MQ=255)                                                                                         tCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTa                                                                          <  1:80938/131‑1 (MQ=255)                                                                                         tCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGc                                                        >  3:360304/1‑149 (MQ=255)                                                                                              gACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGt                                                                   <  6:272066/133‑1 (MQ=255)                                                                                              gACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGt                                                                   >  5:272066/1‑133 (MQ=255)                                                                                                 ccTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAgg                                                                    >  2:35394/1‑129 (MQ=255)                                                                                                 ccTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGc                                                <  2:369691/149‑1 (MQ=255)                                                                                                 ccTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGCATAGCGCGCAGGTCAAGGTCCTTATGAAgg                                                                    <  1:35394/129‑1 (MQ=255)                                                                                                  cTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGCACCATACGCTGCCAGGcc                                               >  4:41694/1‑149 (MQ=255)                                                                                                            aaCGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGcc                                                    <  5:212179/134‑1 (MQ=255)                                                                                                            aaCGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGcc                                                    >  6:212179/1‑134 (MQ=255)                                                                                                            aaCGCCGCCAAGCAGCTTAAGCGCCTTTCGACGGCG‑CAGGCGCTGCTTGCTCGTATCAAGTGACTGCAATGCCAGCCCCGCACCCGGGATAGCGCGAAGGTGAAGGTCCTTATGAATGTGAACCATACGCTGCCAGGCCTGTTCGttt                                      >  5:259313/1‑148 (MQ=255)                                                                                                             aCGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTc                                    <  3:70637/149‑1 (MQ=255)                                                                                                               gccgccAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTCgt                                  <  6:83075/149‑1 (MQ=255)                                                                                                                  gccAAGCAGCTTCAGCGCCTGTCGACGGCA‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTCgtggt                               <  7:234093/149‑1 (MQ=255)                                                                                                                   ccAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTCgtggtg                              >  7:279912/1‑149 (MQ=255)                                                                                                                    cAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAg                                                  <  4:173378/128‑1 (MQ=255)                                                                                                                          ccagcAGCGCCTG‑CGCCGTCGACAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTCGTGGTGGctgctg                       >  6:20027/5‑149 (MQ=255)                                                                                                                           cTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTCGTGGTGGCtgctgc                      >  1:365367/1‑149 (MQ=255)                                                                                                                            agcAGCGCCTG‑CGCCGTCGACAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTCgt                                  <  1:241788/134‑1 (MQ=255)                                                                                                                            agcAGCGCCTG‑CGCCGTCGACAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGCTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTCgt                                  >  2:241788/3‑136 (MQ=255)                                                                                                                             tCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTCGTGGTGGCTGCTgcgc                    >  3:163349/1‑149 (MQ=255)                                                                                                                                 cgcCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCTGGCCTGTTCGTTTTCGTGGTGGCTGCTGCGCCAtt                <  4:163349/149‑1 (MQ=255)                                                                                                                                  gcCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTCGTGGTGGCTGCTGCGCCATTg               >  6:129098/1‑149 (MQ=255)                                                                                                                                       tCGACGTCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTCGTGGTGGCTGCTGCGCCATTgcgcgc          <  8:170046/149‑1 (MQ=255)                                                                                                                                       tCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTCGTGGTGGCTGCTGCGCCATTgcgcg           >  4:313555/1‑148 (MQ=255)                                                                                                                                       tCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTCGTGGTGGCTGCTGCGCCATTgcgc            <  7:311370/147‑1 (MQ=255)                                                                                                                                         gACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGAGCGCAGGTCAATGTCCTTATTAATGTGAAACATACGCTGCCAGGCCTGTTCGTTTTCGTGGTGGCTGCTGCGCCATTGCGCGCAc        <  6:259313/149‑1 (MQ=255)                                                                                                                                             gcg‑cAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTCGTGGTGGCTGCTGCGCCATTGCGCGCACtgtt    <  5:187072/149‑1 (MQ=255)                                                                                                                                              cg‑cAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTCGTGGTGGCTGCTGCGCCAtt                >  6:130035/1‑136 (MQ=255)                                                                                                                                              cg‑cAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTCGTGGTGGCTGCTGCGCCAtt                <  5:130035/136‑1 (MQ=255)                                                                                                                                               g‑cAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTCGTGGTGGCTGCTGCGCCATTGCGCGCACtgttgt  <  8:203646/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     GCAGGGTGAAGACGCGTCGCTCGCCCGTGCCGGTGGCA‑TAGTCTGACGTCCAGGCACTCACAGTATCGAGGTCTTTGGCCAGCCAGCTCGCCGACCCTGCCATGACAACGCCGCCAAGCAGCTTCAGCGCCTGTCGACGGCG‑CAGGCGCTGCTGGCTGGTTTCCAGTGTCTGCAATGCCAGCCCGGCACCCGGGATAGCGCGCAGGTCAAGGTCCTTATGAAGGTGAACCATACGCTGCCAGGCCTGTTCGTTTTCGTGGTGGCTGCTGCGCCATTGCGCGCACTGTTGT  >  NC_002947/185231‑185520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑