| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,499,506:1 | +C | 100% | intergenic (+83/‑81) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,506 | 1 | . | C | 95.7% | 137.6 / 0.3 | 47 | intergenic (+83/‑81) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (20/25); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑T‑CGGGCA‑CG‑CCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGC > NC_002947/1499394‑1499648 | aaCGACGCCTTGGCACCCCACCAGCACGGCGGCTCCCAACGGGTGGGCGCGGGCGTCCCGGCTAAACAGGCGCGGCGGTGCATGGCACGGGTTTTGCCGGTGTGC‑G‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCggg < 2:301712/147‑1 (MQ=12) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAAAAGGCGCCGCGGTGCATGGCACCGGCTTTGGCGGTGT‑TAG‑CGGGCACCC‑CCG‑CGCCCCCCGACTGAGTGCCATTAATCGGGCCGAt > 2:279114/1‑149 (MQ=21) aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATc < 6:389534/149‑1 (MQ=21) ccTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TAG‑CGGGCA‑CGCCCG‑CTCCCACAGACGGATTGCCATTAAGCGGGCCGATCGc > 7:114347/1‑148 (MQ=21) accacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTTGCCGCGAAACAGGCGCCCCGGTGCATGGCACCGGCGTTTCCGGTGT‑TAG‑CGGGCACCC‑CCG‑CTCCCACAGACTGATTTGCATTTATCGGGCCGATAGCGACAGTGa > 1:20524/1‑149 (MQ=16) ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGCCACCGGCTTTGCGGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAc < 3:327613/149‑1 (MQ=21) agccgGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCGTCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGc < 1:279114/149‑1 (MQ=21) gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TAG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGAATGCCAATTAGCGGGCCTATCGCTAAAGTGACGGCCCGGTTTttgttg > 1:55164/1‑149 (MQ=18) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGc < 6:281621/149‑1 (MQ=21) ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACACGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTc > 3:92515/1‑149 (MQ=21) aaaCGGTGGGAGCGGGCGTGCCCGCGAAAAACGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TAG‑CGGGCA‑CGCCCG‑CGCCCACAGACAGAATTCCATTAAGCGGGGCTATGGCGACAGGGACGGCCCCTTTTTTGTTGGCACGc > 2:91235/1‑149 (MQ=12) gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 3:124719/149‑1 (MQ=25) gggCGTGCCCTCGAAACAGGCGCCGGGGGGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa < 8:264107/149‑1 (MQ=37) gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑TGCGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc > 4:46093/1‑149 (MQ=37) gCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt < 2:20524/149‑1 (MQ=37) gCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 7:118861/1‑149 (MQ=37) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 8:242905/1‑148 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCAGGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 3:162970/149‑1 (MQ=37) gAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCAGTTTTTTCTTGCCTCGCTAACGAATCATGAATCCCTTAGAAc > 4:321986/1‑149 (MQ=25) aaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAGGACTCCCTTAGAACg > 2:30835/1‑148 (MQ=37) ggCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 7:242905/149‑1 (MQ=37) cgccgcGGTGCATGGCACCGGCTTTGCGGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCaa < 3:58649/149‑1 (MQ=37) gccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 5:276654/1‑149 (MQ=37) ccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc < 4:92515/149‑1 (MQ=37) gcgGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 8:324725/1‑146 (MQ=37) cATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 4:271625/1‑148 (MQ=38) aTGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACgg > 3:30067/1‑85 (MQ=17) aTGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACgg < 4:30067/85‑1 (MQ=17) aTGGCACCGGCTTGCCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa < 5:87569/149‑1 (MQ=38) tggcaccGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaaa < 7:324725/143‑1 (MQ=38) caccGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGt > 8:176221/4‑149 (MQ=38) accGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTc > 6:150716/3‑149 (MQ=38) accGGCTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTc > 2:357189/3‑149 (MQ=38) ccgtcTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTcc < 1:91235/145‑1 (MQ=39) cTTTGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAc < 8:118861/149‑1 (MQ=39) tttGCCGGTGT‑TCG‑CGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAct > 3:352398/1‑149 (MQ=39) gtgttcgcGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa < 3:321986/142‑1 (MQ=255) tgttcgcGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAt > 1:149272/7‑149 (MQ=255) tgttcgcGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAt < 8:114347/143‑1 (MQ=255) ttcgcGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt > 7:133443/5‑92 (MQ=39) ttcgcGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt < 8:133443/88‑1 (MQ=39) ttcgcGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCaa > 4:58065/5‑117 (MQ=255) ttcgcGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCaa < 3:58065/113‑1 (MQ=255) ttcgcGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGa < 5:150716/145‑1 (MQ=255) tcgcGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGa < 1:357189/145‑1 (MQ=255) cGGGCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcgcg < 7:176221/149‑1 (MQ=255) ggCA‑CGCCCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACgcggc < 5:13856/149‑1 (MQ=255) | AACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑T‑CGGGCA‑CG‑CCG‑CTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGC > NC_002947/1499394‑1499648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |