| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,900,704:1 | +T | 6.6% | coding (1255/4026 nt) | PP_1703 → | assimilatory nitrate reductase/sulfite reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,900,704 | 1 | . | T | 6.6% | 241.5 / 10.2 | 61 | coding (1255/4026 nt) | PP_1703 | assimilatory nitrate reductase/sulfite reductase |
| Reads supporting (aligned to +/- strand): ref base . (32/25); new base T (2/2); total (34/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01 | |||||||||||
CACCCGGCAGCCTGCGCAGCGAGGGGGGCGAGGGTACGGTGGCGCTGTTCGGGCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGTGTGATGATCAACAGCGAGCGCAACCTGACCCTGATGCCACG > NC_002947/1900561‑1900852 | cACCCGGCAGCCTGCGCAGCGAGGGGGGCGAGGGTACGGTGGCGCTGTTCGGGCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑Tgcg > 5:112479/1‑148 (MQ=255) cAGCCTGCGCAGCGAGGGGGGCGAGGGTACGGTGGCGCTGTTCGGGCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCg < 1:378300/149‑1 (MQ=255) cTGCGCAGCGAGGGGGGCGAGGGTACGGTGGCGCTGTTCGGGCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCCTTGCGC‑AGGCCgt > 3:245133/1‑145 (MQ=255) cTGCGCAGCGAGGGGGGCGAGGGTACGGTGGCGCTGTTCGGGCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCCTTGCGC‑AGGCCgt < 4:245133/146‑2 (MQ=255) gcgcAGCGAGGGGGGCGAGGGTACGGTGGCGCTGTTCGGGCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTgg > 4:77271/1‑149 (MQ=255) cgcAGCGAGGGGGGCGAGGGTACGGTGGCGCTGTTCGGGCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTg < 5:30419/147‑1 (MQ=255) tACGGTGGCGCTGTTCGAGCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑Tgc > 7:85486/1‑113 (MQ=255) tACGGTGGCGCTGTTCGAGCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑Tgc < 8:85486/113‑1 (MQ=255) ggTGGCGCTGTTCGGGCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTAccc > 7:244912/1‑134 (MQ=255) ggTGGCGCTGTTCGGGCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTAccc < 8:244912/134‑1 (MQ=255) ggCGCTGTTCGGGCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGaca < 3:247796/149‑1 (MQ=255) ttCGGGCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAaac < 8:8790/149‑1 (MQ=255) ttCGGGCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAaac < 3:77271/149‑1 (MQ=255) ttCGGGCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAaac > 3:344378/1‑149 (MQ=255) ttCGGGCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAaac > 3:338866/1‑149 (MQ=255) cGGGCAGATGCAAGCCGGTGAAGTGAATGCCTGCTGGGTCATCTGCAGCTACCCGGTGGCCAGTGTCGCCAACGGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACAcc < 2:227889/142‑1 (MQ=255) cGGGCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACAcc > 1:227889/1‑142 (MQ=255) gggCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTcc < 6:153338/133‑1 (MQ=255) gggCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTcc > 5:153338/1‑133 (MQ=255) tGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGaa < 3:243634/101‑1 (MQ=255) tGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGaa > 4:243634/1‑101 (MQ=255) gAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACg > 1:318230/1‑149 (MQ=255) gAAAGCCGGTGAAGTGAAAGCCTGCTGCGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGAGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACg > 3:140243/1‑149 (MQ=255) aaGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGcc > 5:159183/1‑149 (MQ=255) aGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGcc > 2:74820/1‑148 (MQ=255) gtgaagtgaaAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATc > 4:66292/1‑149 (MQ=255) aagtgaaAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCctg < 2:124102/149‑1 (MQ=255) gtgaaAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCctgct > 1:92315/1‑149 (MQ=255) aGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTgccgg < 1:74820/149‑1 (MQ=255) gctgGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCcgcg > 3:213629/1‑149 (MQ=255) tCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTgg > 1:360787/1‑149 (MQ=255) aTCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTggg < 3:66292/148‑1 (MQ=255) gcagcaACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCg > 5:342940/1‑147 (MQ=255) cagcaACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAg > 2:162995/1‑149 (MQ=255) agcaACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGCCACCGAAACCCACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAgg > 4:269368/1‑149 (MQ=255) aaCCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGc > 4:143513/1‑147 (MQ=255) tGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACTCCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGtgtg < 4:140243/149‑1 (MQ=255) tGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGtgtg < 6:159183/149‑1 (MQ=255) cAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTa < 3:277248/59‑1 (MQ=255) cAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTa > 4:277248/1‑59 (MQ=255) gtgtCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGTGTGATGATc < 2:92315/149‑1 (MQ=255) tCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCctg > 3:183175/1‑104 (MQ=255) tCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCctg < 4:183175/104‑1 (MQ=255) gCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGTGTGATGATCAACag > 3:277071/1‑149 (MQ=255) aaCCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGTGTGATGATCAACagc > 6:62949/1‑147 (MQ=255) ccGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGgtg < 8:282278/131‑1 (MQ=255) ccGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGgtg > 7:282278/1‑131 (MQ=255) aGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGTGTGATGATCAACAGCGAGCGCAAc < 4:344378/149‑1 (MQ=255) aGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGTGTGATGATCAACAGCGAGCGCAAc < 4:338866/149‑1 (MQ=255) aGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGTGTGATGATCAACAGCGAGCGCAAc > 8:67741/1‑149 (MQ=255) cAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGTGTGATGATCAACAGCGAGCGCAACCt > 3:321724/1‑149 (MQ=255) cAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGTGTGATGATCAACAGCGAGCGCAACCt > 1:336673/1‑149 (MQ=255) aGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGTGtgatga > 4:242073/1‑128 (MQ=255) aGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGTGtgatga < 3:242073/128‑1 (MQ=255) gTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGTGTGATGATCAACAGCGAGCGCAACCTGAc < 6:112479/149‑1 (MQ=255) tGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGTGTGATGATCAACAGCGAGCGCAACCTGAcc > 2:121549/1‑149 (MQ=255) tGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGTGTGATGATCAACAGCGAGCGCAACCTGAcc < 4:213629/149‑1 (MQ=255) gATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGTGTGATGATCAACAGCGAGCGCa > 3:197154/1‑140 (MQ=255) gATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGTGTGATGATCAACAGCGAGCGCa < 4:197154/140‑1 (MQ=255) ttcGGCCTTGCGC‑AGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAgg > 2:158063/3‑112 (MQ=255) ttcGGCCTTGCGC‑AGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAgg < 1:158063/110‑1 (MQ=255) c‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGTGTGATGATCAACAGCGAGCGCAACCTGACCCTGATGCCAcg > 1:373842/1‑149 (MQ=255) | CACCCGGCAGCCTGCGCAGCGAGGGGGGCGAGGGTACGGTGGCGCTGTTCGGGCAGATGAAAGCCGGTGAAGTGAAAGCCTGCTGGGTCATCTGCAGCAACCCGGTGGCCAGTGTCGCCAACCGTCAGCAAGTGATCGACGGCC‑TGCGCAAGGCCGAACTGGTCATTACCCAGGACGCCTTCCTCGACACCGAAACCAACCGTTACGCCGACATCCTGCTGCCGGCCGCGCTGTGGGCCGAAGGCGAGGGTGTGATGATCAACAGCGAGCGCAACCTGACCCTGATGCCACG > NC_002947/1900561‑1900852 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |