| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,932,638:1 | +C | 100% | intergenic (‑105/+45) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 95.8% | 151.2 / ‑0.1 | 48 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (24/21); total (27/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑C‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTC > NC_002947/1932500‑1932773 | ctccGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAgg > 7:249921/4‑149 (MQ=255) ttggAAGAGGCTCTGGACCTTGGACAGCGGGCTTGCAGGTCTGACGAAAATAGCAGTAAACGCGGGGGCCCGGTTG‑CG‑‑‑GCCCATCGCGGGGAAGCCCGCGGCGACCAAGGTTATGCGGCCCCGGGAGGCGACGGC‑GTGCC‑CGCGGAggg > 6:82955/4‑149 (MQ=255) tCGAAGAGGATCTGGACATTCGACAGTGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTACTACAAAGGATATGCGTCCCCTGTACGAGCGGGC‑TTGAC‑CGCGAAGGGc > 1:204525/1‑149 (MQ=38) tCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGc > 2:260431/1‑149 (MQ=255) cgactcggATCTGCACATTCGACAGCGGCATTCCCGGGCTCACCAAAATAGCCGTAAACGCGGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAggg < 8:249921/141‑1 (MQ=25) gAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTg > 7:250901/1‑149 (MQ=255) gagGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGGTATGCGTGCCCAGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGc > 3:229006/1‑147 (MQ=38) gATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAgg > 2:47682/1‑148 (MQ=255) gATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCGCCTACCAAGGATTTGCGTGCCCTGGAGGAGCGGGC‑GTGCC‑CGCGAAGGGCTGCAAGGc > 6:224572/1‑149 (MQ=38) cATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCAGTAGGAGCGGGC‑TTG‑CACGCGAAGGGCTGCAAGGCAGCCCCa > 2:279562/1‑148 (MQ=255) aTTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCaaa > 7:70118/1‑149 (MQ=255) aTTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCaaa > 2:98301/1‑149 (MQ=255) cAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCg < 4:119998/149‑1 (MQ=255) gaaGGTCTCACGAAAAAATCACTAATAACTGGGGCCGCTTTGCCGCATGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAc < 1:126482/146‑1 (MQ=21) gCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGa > 8:24960/1‑149 (MQ=255) gTCTCACTAAAATACCAGTAACCGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAACGTATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAg < 2:279413/149‑1 (MQ=37) gTCTCACTAAAATACCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGTATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAg < 7:28333/149‑1 (MQ=37) ctcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 5:89976/132‑1 (MQ=255) ctcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCGGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc > 6:89976/1‑132 (MQ=255) tcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTACTACAAAGGATATTCGTGCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAAGCAGCCCCAAATTGTGGACTACGCGCAtacacg > 8:164116/1‑143 (MQ=25) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc > 8:156199/1‑149 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc > 6:156311/1‑149 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑c‑ccgcga < 5:83823/102‑6 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑c‑ccgcga > 6:83823/1‑97 (MQ=255) gAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCg > 5:274482/1‑149 (MQ=255) gCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGt < 8:250901/149‑1 (MQ=255) aaaCGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg < 8:70118/148‑1 (MQ=255) aaCGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTCCAAAGCATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg < 7:164116/147‑1 (MQ=255) ggcgggggCGGTTTTG‑CG‑‑‑GCCCTGCGCGGCCACGCCCGCTCCTAAAAAGTATCTGCGTCCCGTGTAGGCGGGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCa < 7:127780/145‑1 (MQ=255) gCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAg > 7:223466/1‑149 (MQ=255) cTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAACCCGCAACGAAAAAAGATATGCGTGCCCGGTAAGAGCGGGCTTTG‑C‑CGCCCAGGGCAGCAAAGGAGCCCCAAAATTTAGACAAAGCGGGAGCGCGAAACCGCAGGGTGATGttgat > 2:334621/1‑145 (MQ=255) gCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTc > 5:88910/1‑146 (MQ=255) tG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCg < 1:260431/147‑1 (MQ=255) tG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGa < 1:98301/148‑1 (MQ=255) tG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGa < 6:274482/148‑1 (MQ=255) g‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGag > 6:83772/1‑148 (MQ=255) cG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagc < 5:83772/148‑1 (MQ=255) cccTTCGCGGGCAAGCCCGCGCCTACAAAGGATATGCGTCCCCGGTAGGAGCGGGC‑GTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCt > 4:269839/1‑149 (MQ=39) gCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCa < 1:47682/149‑1 (MQ=255) cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAg > 6:228913/1‑149 (MQ=255) cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAg > 4:299921/1‑149 (MQ=255) cGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCt < 3:299921/149‑1 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata > 3:113700/1‑149 (MQ=255) tatGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatcat < 4:113700/149‑1 (MQ=255) atGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc > 2:350513/1‑108 (MQ=255) atGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc < 1:350513/108‑1 (MQ=255) tGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc < 5:228913/148‑1 (MQ=255) cTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAt > 8:41361/1‑149 (MQ=255) ggAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc < 2:240834/148‑1 (MQ=255) ggAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc < 5:156311/148‑1 (MQ=255) | CTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTG‑CG‑‑‑GCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑C‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTC > NC_002947/1932500‑1932773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |