Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_002947	2,958,780	Δ61 bp	8.4%	intergenic (+46/+36)	PP_2589 → / ← PP_2590	aldehyde dehydrogenase family protein/ferric siderophore receptor

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 2958779	49 (0.830)	4 (0.070)	4/244	NT	8.4%	intergenic (+45/+97)	PP_2589/PP_2590	aldehyde dehydrogenase family protein/ferric siderophore receptor
	?	NC_002947	2958841 =	41 (0.730)					intergenic (+107/+35)	PP_2589/PP_2590	aldehyde dehydrogenase family protein/ferric siderophore receptor

CGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAAC  >  NC_002947/2958631‑2958924                                                                                                                                                      |                                                                                                                                                  cGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGC                                                                                                                                                   <  5:146711‑M1/149‑1 (MQ=255)       gTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCATCGTCTGCTTCGCGGGCATGCCCGCTCCca                                                                                                                                              >  1:156669/1‑148 (MQ=255)         gggggCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAg                                                                                                                                           >  7:175333/1‑149 (MQ=255)         gggggCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAg                                                                                                                                           <  3:163946/149‑1 (MQ=255)             gCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTc                                                                                                                                       <  7:3964/149‑1 (MQ=255)                aGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGc                                                                                                                                    <  3:108935/149‑1 (MQ=255)                  cAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGcac                                                                                                                                  <  3:173782/149‑1 (MQ=255)                     tCGGGGTTTGGCCGCGATCTATCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCGGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCGCGCCCGCTCCCACAGGTCCAGCACaac                                                                                                                               >  1:212276/1‑148 (MQ=39)                          gTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAg                                                                                                                                     <  4:76065/138‑1 (MQ=255)                          gTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAg                                                                                                                                     >  3:76065/1‑138 (MQ=255)                             tGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCATCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAgg                                                                                                                        <  2:156669/148‑1 (MQ=255)                                 cgcgATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTtgt                                                                                                                   >  1:60958/1‑149 (MQ=255)                                  gcgATCTGTCACTGCATTCGTTAGACAAGTTTTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTTATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTtgtg                                                                                                                  <  8:314762/149‑1 (MQ=255)                                      tCTGTCACTGCTTTCGTTCTACAAGTATTCGCAACTCAAGACTACCTGGTACCAGTTGCGG‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTg                                                                                                               <  4:2881/148‑1 (MQ=255)                                          tCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCGCGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAAc                                                                                                            <  7:348738/147‑1 (MQ=25)                                          tCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAACCCTCAGACTTGTGCTGAAc                                                                                                            <  3:250671/147‑1 (MQ=38)                                          tCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCGGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCGCGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTGAAcc                                                                                                           <  2:212276/148‑1 (MQ=25)                                            aCTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCtgt                                                                                                        <  8:175333/149‑1 (MQ=255)                                            aCTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCtgt                                                                                                        <  8:204448/149‑1 (MQ=255)                                              tGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGtt                                                                                                                     >  8:83979/1‑134 (MQ=255)                                              tGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGtt                                                                                                                     <  7:83979/134‑1 (MQ=255)                                                cATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTgg                                                                                                      >  1:284637/1‑148 (MQ=255)                                                cATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTgg                                                                                                      >  8:28659/1‑147 (MQ=255)                                                         gACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGgcgcg                                                                                           >  4:173505/1‑149 (MQ=255)                                                            aaGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGccc                                                                                        >  1:62644/1‑149 (MQ=255)                                                                 ttCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCcgcg                                                                                     >  2:29209/1‑147 (MQ=255)                                                                 ttCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCcgcg                                                                                     >  5:289800/1‑147 (MQ=255)                                                                    gCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAagag                                                                                >  3:342543/1‑149 (MQ=255)                                                                    gCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAagag                                                                                >  4:37134/1‑149 (MQ=255)                                                                      aaCTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAgg                                                                               >  7:277720/1‑148 (MQ=255)                                                                      aaCTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAgg                                                                               <  8:277720/148‑1 (MQ=255)                                                                        cTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCACGCCCGCTCCCACAGGTCCAGCACAACCCTCAGACTTGTGCTga                                                                                                              <  5:272988/115‑2 (MQ=21)                                                                         tCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCgaagaggccgtatctggcgccgcggcgggacccggt                                                                                                               >  5:195337‑M1/1‑77 (MQ=255)                                                                         tCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCgaagaggccgtatctggcgccgcggcgggacccggt                                                                                                               <  6:195337‑M1/113‑37 (MQ=255)                                                                         tCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCg                                                                            >  6:369207/1‑148 (MQ=255)                                                                           aaGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACCGGTTCCGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGt                                                                           <  7:109391/147‑1 (MQ=255)                                                                            aGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTAta                                                                        >  3:1053/1‑148 (MQ=255)                                                                                caccTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACaa                                                                                                                                <  2:232883/89‑1 (MQ=255)                                                                                caccTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACaa                                                                                                                                >  1:232883/1‑89 (MQ=255)                                                                                         ccAGTTGCGC‑GGCTGATGTTTTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACCGGTTCAGCACACGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCgcggcg                                                           <  2:284637/149‑1 (MQ=255)                                                                                                      cTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTa                                                <  7:268384/148‑1 (MQ=255)                                                                                                      cTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAg                                               <  5:369207/149‑1 (MQ=255)                                                                                                       tGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCgaagaggccgtatctggcgccgcggcgggacccggttagaagtccacgctagccgaaagctgataggt                                                                               <  6:53787‑M1/116‑69 (MQ=255)                                                                                                       tGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCgaagaggccgtatctggcgccgcggcgggacccggttagaagtccacgctagccgaaagctgataggt                                                                               >  5:53787‑M1/1‑48 (MQ=255)                                                                                                       tGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCGGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTa                                                >  8:268384/1‑147 (MQ=255)                                                                                                             aTTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCa                                        >  1:343039/1‑149 (MQ=255)                                                                                                              ttGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCAc                                       >  7:213480/1‑149 (MQ=255)                                                                                                              ttGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCAc                                       >  4:338192/1‑149 (MQ=255)                                                                                                                    aTTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTACACGCTAg                                  <  7:28659/148‑1 (MQ=255)                                                                                                                                 tcTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGCCCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAg                    >  7:123247/1‑149 (MQ=255)                                                                                                                                 tcTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAg                    <  2:62644/149‑1 (MQ=255)                                                                                                                                 tcTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCACCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAATTCCACCCTAGCCCAAATCAGATAg                    >  8:75867/1‑149 (MQ=38)                                                                                                                                   tGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGt                  <  3:307173/149‑1 (MQ=255)                                                                                                                                         gcgGGCAGGCCCGCTCCCACCGGTTCAGCACAAGTCTGCGGGTGGGGCGGGACCTGGGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCgcgg            <  3:37134/149‑1 (MQ=38)                                                                                                                                                tGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGccccc     <  2:60958/149‑1 (MQ=255)                                                                                                                                                   ccGCgaagaggccgtatctggcgccgcggcgggacccggttagaagtccac                                                                                                    >  1:245857‑M1/1‑4 (MQ=255)                                                                                                                                                   ccGCgaagaggccgtatctggcgccgcggcgggacccggttagaagtccac                                                                                                    <  2:245857‑M1/51‑48 (MQ=255)                                                                                                                                                   ccGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGTCCCGGTTAGAAGTCCCCGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAAc  >  7:250696/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                  CGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGC‑GGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAAC  >  NC_002947/2958631‑2958924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.